Shkbp1 (NM_138676) Mouse Tagged ORF Clone
Lenti ORF clone of Shkbp1 (mGFP-tagged) - Mouse Sh3kbp1 binding protein 1 (Shkbp1)
|Product Name||Shkbp1 (NM_138676) Mouse Tagged ORF Clone|
|Type||Mouse Tagged ORF Clone|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|ORF Size||2112 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||2347 bp|
|RefSeq ORF||2115 bp|
|Gene Summary||Inhibits CBL-SH3KBP1 complex mediated down-regulation of EGFR signaling by sequestration of SH3KBP1. Binds to SH3KBP1 and prevents its interaction with CBL and inhibits translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation (PubMed:21830225).[UniProtKB/Swiss-Prot Function]|
|cDNA Clone Resources|
|MC203620||Shkbp1 (untagged) - Mouse Sh3kbp1 binding protein 1 (Shkbp1), (10ug)||
|MR210149||Shkbp1 (Myc-DDK-tagged) - Mouse Sh3kbp1 binding protein 1 (Shkbp1)||
|MG210149||Shkbp1 (GFP-tagged) - Mouse Sh3kbp1 binding protein 1 (Shkbp1)||
|MR210149L3||Lenti ORF clone of Shkbp1 (Myc-DDK-tagged) - Mouse Sh3kbp1 binding protein 1 (Shkbp1)||