Acsl1 (NM_007981) Mouse Tagged ORF Clone

CAT#: MR210117

Acsl1 (Myc-DDK-tagged) - Mouse acyl-CoA synthetase long-chain family member 1 (Acsl1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acsl1
• Synonyms: Ac; Acas; Acas1; Acs; Fac; Facl2; FACS; LACS 1; LACS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210117 representing NM_007981
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGTCCATGAATTGTTCCGGTATTTTCGAATGCCAGAGCTGATTGACATTCGGCAGTACGTGCGCA
  CCCTTCCAACCAACACCCTCATGGGGTTTGGGGCTTTTGCAGCGCTCACCACCTTCTGGTATGCCACCAG
  GCCTAAGGCCCTGAAGCCACCATGTGACCTCTCCATGCAGTCAGTGGAAATAGCGGGTACCACTGATGGT
  ATTCGAAGATCAGCAGTCCTTGAAGATGACAAGCTCTTGGTGTACTACTACGACGATGTCAGAACCATGT
  ACGATGGCTTCCAGAGGGGGATTCAGGTGTCAAATAATGGTCCTTGTTTAGGTTCTCGGAAGCCAAACCA
  GCCCTATGAGTGGATTTCCTACAAAGAGGTGGCAGAACTGGCTGAGTGCATAGGCTCCGGGCTGATCCAG
  AAGGGGTTCAAGCCTTGCTCCGAGCAGTTCATCGGCCTCTTCTCTCAAAACAGACCCGAGTGGGTGATCG
  TCGAGCAAGGATGCTTCTCTTACTCAATGGTGGTCGTCCCGCTCTATGACACCCTTGGAGCTGACGCCAT
  CACCTACATAGTGAACAAAGCTGAACTCTCTGTGATTTTTGCTGACAAGCCAGAAAAAGCCAAACTCTTA
  TTAGAAGGTGTAGAAAACAAGTTAACACCATGCCTTAAAATCATAGTCATCATGGACTCCTACGGCAGTG
  ATCTGGTGGAACGAGGCAAGAAGTGTGGGGTGGAAATCATCAGCCTCAAAGCTCTGGAGGACCTTGGAAG
  AGTGAACAGAGTGAAGCCCAAGCCTCCAGAACCCGAAGATCTTGCGATAATTTGTTTCACAAGTGGAACT
  ACAGGCAACCCCAAAGGAGCAATGATCACTCACCAAAACATTATAAACGACTGCTCAGGTTTTATAAAAG
  CAACAGAGAGTGCATTCATCGCTTCCACAGATGATGTGCTGATATCTTTCTTGCCTCTCGCCCATATGTT
  TGAGACCGTTGTAGAGTGTGTAATGCTGTGTCATGGAGCTAAGATAGGATTTTTCCAAGGAGATATCAGG
  CTGCTTATGGACGACCTCAAGGTGCTTCAGCCCACCATCTTCCCTGTGGTTCCCAGGCTGCTGAACCGGA
  TGTTCGACAGAATTTTTGGACAAGCAAACACTTCCTTGAAGCGATGGCTGTTGGACTTTGCCTCCAAAAG
  GAAAGAGGCGGAGCTTCGCAGTGGCATCGTCAGAAACAACAGCCTGTGGGATAAACTCATCTTCCACAAG
  ATACAGTCGAGCCTGGGTGGGAAAGTCCGGCTGATGATCACAGGAGCAGCCCCGGTGTCTGCCACAGTGC
  TGACGTTTCTGAGGACAGCGCTCGGCTGCCAGTTCTATGAAGGCTACGGACAGACCGAGTGCACTGCTGG
  TTGCTGCCTGAGCTTGCCCGGAGACTGGACGGCAGGCCATGTTGGAGCCCCCATGCCTTGCAATTATGTA
  AAGCTTGTGGATGTGGAAGAAATGAATTACCTGGCATCCAAGGGCGAGGGTGAGGTGTGTGTGAAAGGGG
  CAAATGTGTTCAAAGGCTACTTGAAAGACCCAGCAAGAACAGCTGAAGCCCTGGATAAAGATGGCTGGTT
  ACACACGGGGGACATTGGAAAATGGCTGCCAAATGGCACCTTGAAGATTATCGACAGGAAAAAGCACATA
  TTTAAACTAGCCCAAGGAGAGTACATAGCACCAGAAAAGATTGAAAATATCTACCTGCGGAGTGAAGCCG
  TGGCCCAGGTGTTTGTCCACGGAGAAAGCTTGCAGGCCTTTCTCATAGCAGTTGTGGTACCCGACGTTGA
  GAGCCTACCGTCCTGGGCACAGAAGAGAGGCTTACAAGGGTCCTTCGAAGAACTGTGCAGGAACAAGGAT
  ATCAATAAAGCTATCCTGGACGACTTGTTGAAACTTGGGAAGGAAGCCGGTCTGAAGCCATTTGAACAGG
  TCAAAGGCATTGCTGTGCACCCGGAATTATTTTCTATTGACAACGGCCTTCTGACTCCAACACTGAAGGC
  GAAGAGGCCAGAGCTACGGAACTATTTCAGGTCGCAGATAGATGAACTGTACGCCACCATCAAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210117 representing NM_007981
  Red=Cloning site Green=Tags(s)
  
  MEVHELFRYFRMPELIDIRQYVRTLPTNTLMGFGAFAALTTFWYATRPKALKPPCDLSMQSVEIAGTTDG
  IRRSAVLEDDKLLVYYYDDVRTMYDGFQRGIQVSNNGPCLGSRKPNQPYEWISYKEVAELAECIGSGLIQ
  KGFKPCSEQFIGLFSQNRPEWVIVEQGCFSYSMVVVPLYDTLGADAITYIVNKAELSVIFADKPEKAKLL
  LEGVENKLTPCLKIIVIMDSYGSDLVERGKKCGVEIISLKALEDLGRVNRVKPKPPEPEDLAIICFTSGT
  TGNPKGAMITHQNIINDCSGFIKATESAFIASTDDVLISFLPLAHMFETVVECVMLCHGAKIGFFQGDIR
  LLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTSLKRWLLDFASKRKEAELRSGIVRNNSLWDKLIFHK
  IQSSLGGKVRLMITGAAPVSATVLTFLRTALGCQFYEGYGQTECTAGCCLSLPGDWTAGHVGAPMPCNYV
  KLVDVEEMNYLASKGEGEVCVKGANVFKGYLKDPARTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHI
  FKLAQGEYIAPEKIENIYLRSEAVAQVFVHGESLQAFLIAVVVPDVESLPSWAQKRGLQGSFEELCRNKD
  INKAILDDLLKLGKEAGLKPFEQVKGIAVHPELFSIDNGLLTPTLKAKRPELRNYFRSQIDELYATIKI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007981
• ORF Size: 2097 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007981.4, NP_032007.2
• RefSeq Size: 3891 bp
• RefSeq ORF: 2100 bp
• Locus ID: 14081
• UniProt ID: P41216
• Cytogenetics: 8 B1.1
• MW: 78.4 kDa
• Gene Summary: The protein encoded by this gene belongs to a family of acyl coenzyme A synthetase proteins, which convert long chain fatty acids to acyl CoA products via an ATP-dependent pathway. This enzyme is enriched in heart, liver and adipose tissue, where it functions in lipid synthesis and mitochondrial and peroxisomal beta-oxidation. In addition, it is expressed in monocytes and macrophages where it appears to have a functionally distinct role in mediating inflammatory and innate immune responses. A pseudogene of this gene is found on chromosome 5. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]