Slc34a2 (NM_011402) Mouse Tagged ORF Clone

CAT#: MR210105

Slc34a2 (Myc-DDK-tagged) - Mouse solute carrier family 34 (sodium phosphate), member 2 (Slc34a2)

Product Images

Circular map for MR210105

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc34a2
• Synonyms: AA536683; D5Ertd227e; NaPi-2b; Npt2b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210105 representing NM_011402
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCTTGGCCCGAGTTGGAAAATGCCCAGCCCAACCCCGGTAAATTCATCGAGGGGGCCTCTGGAC
  CACAATCGAGCATACCTGCCAAAGACAAGGAGGCCAGCAAAACAAATGACAACGGGACTCCTGTAGCCAA
  GACTGAACTGCTACCCTCCTATTCCGCCCTGGTTCTCATAGAGGAGCATCCCGAGGGGACGGACCCCTGG
  GACCTGCCTGAACTCCAGGACACTGGGATCAAATGGTCAGAGAGAGACACCAAAGGGAAGACGCTCTGCA
  TTTTCCAGGGGGTTGGGAAGTTCATTCTTCTCCTGGGGTTTCTCTACTTGTTCGTGTGCTCCCTGGATGT
  ACTGAGCAGCGCCTTCCAGCTTGTTGGAGGCAAAGTGGCCGGACAGTTCTTCAGCAACAACTCCATCATG
  TCTAATCCTGTGGCTGGACTGGTGATAGGGGTTCTGGTGACAGTCATGGTTCAAAGCTCCAGCACCTCTT
  CCTCCATCATCGTCAGCATGGTTGCTTCTTCATTGCTGACGGTTCGGGCTGCCATCCCCATCATCATGGG
  CGCTAACATTGGAACCTCCATCACCAACACGATCGTGGCGCTTATGCAGGCAGGAGACAGAAATGAGTTC
  AGAAGGGCATTTGCAGGAGCCACTGTTCATGACTTCTTCAACTGGCTCTCTGTGTTCGTGCTCTTGCCTC
  TGGAGGCTGCCACCCATTACCTGGAGATCCTGACCAACCTTGTGTTAGAAACCTTCAAGTTCCAGAATGG
  AGAAGATGCCCCAGACATTCTGAAAGTCATCACAGACCCCTTCACAAAGCTCATCATCCAGCTGGATAAA
  AAGGTCATCCAACAAATCGCAATGGGTGACTCAGCGGCCCAGAACAAGAGCCTGATCAAGATCTGGTGTA
  AAAGTATAACCAATGTGACTGAGATGAATGTCACCGTCCCCTCGACTGATAACTGCACCTCTCCTTCATA
  TTGCTGGACTGATGGCATACAGACCTGGACCATCCAGAACGTTACCCAAAAGGAGAACATTGCTAAATGT
  CAGCACATCTTCGTGAACTTCAGTCTCCCAGACCTTGCTGTGGGCATCATCTTGCTTACTGTCTCCCTGG
  TGGTCCTCTGTGGCTGTCTGATCATGATAGTCAAGCTGTTGGGTTCTGTGCTCAGGGGACAAGTCGCTAC
  TGTCATCAAGAAGACCCTGAATACTGATTTCCCCTTTCCCTTTGCTTGGCTGACTGGCTACCTGGCCATC
  CTTGTGGGGGCAGGCATGACCTTCATCGTGCAAAGCAGCTCTGTGTTCACGTCTGCCATGACTCCACTCA
  TCGGTATTGGGGTGATCAGCATTGAGAGGGCATATCCACTCACGTTGGGCTCCAACATTGGCACCACCAC
  CACCGCCATTCTGGCTGCTTTAGCCAGCCCAGGCAACACCTTGAGGAGTTCTCTCCAGATTGCCCTGTGC
  CATTTCTTCTTCAACATCTCTGGAATATTGCTTTGGTACCCAATCCCATTCACCCGTCTGCCCATCCGTC
  TGGCTAAAGGCCTAGGCAACATCTCTGCCAAGTATCGCTGGTTCGCAGTCTTCTATCTCATCTTCTTCTT
  CTTTGTGACCCCGCTGACAGTGTTTGGCCTGTCTCTGGCAGGCTGGCCAGTGCTGGTGGGCGTGGGCGTA
  CCCATCATCCTGCTGCTGCTCCTGGTTCTGTGCCTCCGGATGCTGCAGTTCCGCTGCCCTCGCATCTTGC
  CCCTGAAGCTCCGGGACTGGAATTTCCTACCCTTGTGGATGCACTCTCTGAAACCCTGGGACAACGTCAT
  CTCCCTGGCCACCACCTGCTTCCAGAGGCGTTGCTGCTGCTGTTGCCGCGTGTGCTGCCGTGTCTGCTGC
  ATGGTGTGCGGCTGCAAGTGCTGTCGCTGCAGCAAGTGCTGCAGGGACCAGGGAGAGGAAGAGGAGGAGA
  AGGAGCAGGATATCCCGGTCAAGGCTTCTGGGGCCTTTGACAATGCAGCTATGAGCAAAGAGTGCCAAGA
  TGAGGGCAAGGGCCAAGTGGAAGTTCTGAGCATGAAGGCCCTGTCCAACACTACAGTGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210105 representing NM_011402
  Red=Cloning site Green=Tags(s)
  
  MAPWPELENAQPNPGKFIEGASGPQSSIPAKDKEASKTNDNGTPVAKTELLPSYSALVLIEEHPEGTDPW
  DLPELQDTGIKWSERDTKGKTLCIFQGVGKFILLLGFLYLFVCSLDVLSSAFQLVGGKVAGQFFSNNSIM
  SNPVAGLVIGVLVTVMVQSSSTSSSIIVSMVASSLLTVRAAIPIIMGANIGTSITNTIVALMQAGDRNEF
  RRAFAGATVHDFFNWLSVFVLLPLEAATHYLEILTNLVLETFKFQNGEDAPDILKVITDPFTKLIIQLDK
  KVIQQIAMGDSAAQNKSLIKIWCKSITNVTEMNVTVPSTDNCTSPSYCWTDGIQTWTIQNVTQKENIAKC
  QHIFVNFSLPDLAVGIILLTVSLVVLCGCLIMIVKLLGSVLRGQVATVIKKTLNTDFPFPFAWLTGYLAI
  LVGAGMTFIVQSSSVFTSAMTPLIGIGVISIERAYPLTLGSNIGTTTTAILAALASPGNTLRSSLQIALC
  HFFFNISGILLWYPIPFTRLPIRLAKGLGNISAKYRWFAVFYLIFFFFVTPLTVFGLSLAGWPVLVGVGV
  PIILLLLLVLCLRMLQFRCPRILPLKLRDWNFLPLWMHSLKPWDNVISLATTCFQRRCCCCCRVCCRVCC
  MVCGCKCCRCSKCCRDQGEEEEEKEQDIPVKASGAFDNAAMSKECQDEGKGQVEVLSMKALSNTTVF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011402
• ORF Size: 2091 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011402.3, NP_035532.2
• RefSeq Size: 4185 bp
• RefSeq ORF: 2094 bp
• Locus ID: 20531
• UniProt ID: Q9DBP0
• Cytogenetics: 5 28.92 cM
• MW: 76.7 kDa
• Gene Summary: May be involved in actively transporting phosphate into cells via Na(+) cotransport. It may be the main phosphate transport protein in the intestinal brush border membrane. May have a role in the synthesis of surfactant in lungs' alveoli.[UniProtKB/Swiss-Prot Function]