Gucy1a1 (NM_021896) Mouse Tagged ORF Clone

CAT#: MR210068

Gucy1a3 (Myc-DDK-tagged) - Mouse guanylate cyclase 1, soluble, alpha 3 (Gucy1a3)

Product Images

Circular map for MR210068

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gucy1a1
• Synonyms: 1200016O07Rik; sGC-alpha1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210068 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTGCAGGAAGTTCAAGGATCTCAAGATCACAGGGGAGTGTCCTTTCTCCTTACTGGCCCCTGGTC
  AGGTTCCTAAGGAGCCAACAGAGGAGGTGGCTGGAGGCTCTGAGGGCTGCCAGGCTACTCTGCCCATCTG
  CCAGTACTTTCCTGAGAAGAATGCAGAAGGGAGTCTCCCCCAAAGAAAGACAAGCCGCAACAGAGTCTAC
  CTGCACACCCTGGCAGAGAGTATTTGCAAGCTCATCTTCCCAGAGTGTGAGCGACTGAACCTTGCACTTC
  AGAGAACCTTGGCAAAACATAAAATAGAAGAAAACAGGAAATCTTCAGAAAAAGAAGACCTTGAAAAAAT
  AATCGCAGAAGAAGCAATTGCAGCAGGTGCCCCAGTGGAGGCGCTCAAAGACTCTCTGGGCGAGGAGCTG
  TTCAAGATCTGCTATGAGGAAGATGAGCACATTTTGGGCGTGGTTGGCGGCACCCTGAAGGACTTTCTAA
  ATAGCTTCAGCACGCTCCTCAAGCAGAGCAGCCACTGCCAAGAGGCGGAGAGGCGGGGACGACTGGAAGA
  TGCCTCCATCTTATGCCTGGACAAGGACCAGGACTTTCTAAATGTTTACTACTTCTTCCCGAAGAGAACC
  ACAGCCCTGCTTCTCCCTGGTATCATTAAAGCGGCTGCTCGCATACTGTACGAAAGCCACGTGGAGGTGT
  CCCTGATGCCTCCCTGCTTCCGAAGTGACTGTACCGAGTTTGTGAACCAGCCCTATTTGCTCTACTCCGT
  TCATGTGAAGAGCACCAAGCCGTCCCTGTCCCCAGGCAAGCCCCAGTCCTCGCTAGTGATCCCCGCTTCG
  CTCTTCTGCAAGACTTTCCCGTTCCATTTCATGCTGGACCGAGACCTGGCCATCCTGCAGCTGGGTAACG
  GCATCAGAAGGCTGGTGAACAAGAGGGACTTCCAAGGGAAGCCCAACTTTGAAGAGTTCTTTGAAATTCT
  AACTCCCAAAATCAACCAGACATTTAGTGGCATCATGACAATGTTGAACATGCAGTTTGTCATCCGGGTG
  AGGAGATGGGATAACTCGGTGAAGAAATCGTCAAGGGTTATGGATCTCAAAGGTCAAATGATCTACATCG
  TTGAATCCAGTGCCATCTTGTTCTTAGGGTCACCATGTGTGGACAGGCTGGAAGATTTCACAGGACGGGG
  GCTCTATCTGTCCGACATCCCAATTCATAACGCCCTGAGGGATGTTGTCTTGATAGGGGAGCAGGCACGG
  GCTCAAGATGGCCTCAAGAAGAGGTTGGGGAAGCTGAAGGCAACCCTGGAGCATGCCCACCAAGCCCTGG
  AGGAGGAGAAGAAGAGGACAGTGGATCTGCTGTGCTCTATCTTCCCCTCTGAGGTTGCTCAGCAGCTGTG
  GCAAGGACAAATTGTGCAAGCCAAGAAATTCAGCGAGGTCACCATGCTTTTCTCAGATATCGTAGGGTTC
  ACTGCTATCTGCTCTCAGTGTTCACCTCTGCAGGTCATCACGATGCTCAACGCTCTCTACACTCGCTTTG
  ACCAGCAGTGTGGAGAGCTGGATGTCTACAAGGTGGAGACCATCGGGGATGCATATTGTGTGGCAGGTGG
  ATTGCACAGAGAGAGTGACACCCATGCTGTCCAGATAGCACTGATGGCCCTGAAGATGATGGAGCTCTCC
  AATGAGGTCATGTCTCCCCACGGAGAACCTATCAAGATGCGAATTGGACTACATTCTGGATCAGTGTTTG
  CTGGAGTTGTCGGAGTGAAGATGCCCCGGTATTGCCTGTTTGGAAACAATGTCACTCTGGCTAACAAATT
  TGAATCCTGCAGTGTGCCTCGGAAAATCAATGTCAGCCCCACCACATACAGGTTACTCAAAGACTGTCCT
  GGCTTTGTGTTCACCCCGAGATCAAGGGAGGAGCTTCCACCAAACTTCCCTAGTGACATTCCTGGGATCT
  GTCACTTTCTGGATGCTTATCACCATCAAGGACCTAATTCCAAACCATGGTTCCAGGATAAAGATGTGGA
  AGATGGAAACGCCAACTTCTTAGGCAAAGCGTCAGGGATAGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210068 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFCRKFKDLKITGECPFSLLAPGQVPKEPTEEVAGGSEGCQATLPICQYFPEKNAEGSLPQRKTSRNRVY
  LHTLAESICKLIFPECERLNLALQRTLAKHKIEENRKSSEKEDLEKIIAEEAIAAGAPVEALKDSLGEEL
  FKICYEEDEHILGVVGGTLKDFLNSFSTLLKQSSHCQEAERRGRLEDASILCLDKDQDFLNVYYFFPKRT
  TALLLPGIIKAAARILYESHVEVSLMPPCFRSDCTEFVNQPYLLYSVHVKSTKPSLSPGKPQSSLVIPAS
  LFCKTFPFHFMLDRDLAILQLGNGIRRLVNKRDFQGKPNFEEFFEILTPKINQTFSGIMTMLNMQFVIRV
  RRWDNSVKKSSRVMDLKGQMIYIVESSAILFLGSPCVDRLEDFTGRGLYLSDIPIHNALRDVVLIGEQAR
  AQDGLKKRLGKLKATLEHAHQALEEEKKRTVDLLCSIFPSEVAQQLWQGQIVQAKKFSEVTMLFSDIVGF
  TAICSQCSPLQVITMLNALYTRFDQQCGELDVYKVETIGDAYCVAGGLHRESDTHAVQIALMALKMMELS
  NEVMSPHGEPIKMRIGLHSGSVFAGVVGVKMPRYCLFGNNVTLANKFESCSVPRKINVSPTTYRLLKDCP
  GFVFTPRSREELPPNFPSDIPGICHFLDAYHHQGPNSKPWFQDKDVEDGNANFLGKASGID
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021896
• ORF Size: 2076 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021896.3
• RefSeq Size: 4671 bp
• RefSeq ORF: 2076 bp
• Locus ID: 60596
• UniProt ID: Q9ERL9
• Cytogenetics: 3 E3
• MW: 77.6 kDa