Aacs (NM_030210) Mouse Tagged ORF Clone

CAT#: MR209932

Aacs (Myc-DDK-tagged) - Mouse acetoacetyl-CoA synthetase (Aacs)

Product Images

Circular map for MR209932

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aacs
• Synonyms: 2210408B16Rik; SUR5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209932 representing NM_030210
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAAGCTGGCGCGGCTCGAGCGCGAGGAGATCATGGAGTGCCAGGTGATGTGGGAGCCTGACAGCA
  AGAAGGACACGCAGATGGACCGCTTCCGGGCGGCCGTGGGTACCGCCTGCGGCCTGGCGCTTGGGAATTA
  CAATGACTTATACCACTGGTCTGTCCGGTCGTATATGGACTTTTGGGCTGAGTTCTGGAAGTTCAGTGGA
  ATCGTCTACTCACGCATGTATGATGAGGTTGTGGACACATCCAAAGGGATTGCAGATGTCCCCGAGTGGT
  TCAGAGGCAGCCGCCTCAACTACGCAGAGAACCTCCTGCGGCACAAGGAGAACGACAGAGTCGCCCTTTA
  CGTGGCCCGGGAAGGCAGAGAGGAGATCGTGAAGGTGACTTTTGAAGAGCTGCGGCAGCAGGTGGCTCTG
  TTCGCAGCTGCCATGAGGAAGATGGGCGTGAAGAAAGGGGACCGTGTGGTCGGCTATCTACCCAACAGTG
  CGCATGCGGTGGAGGCCATGCTGGCTGCTGCCAGTATTGGGGCCATTTGGAGTTCTACCTCGCCGGACTT
  TGGTGTAAATGGTGTCCTGGACCGCTTTTCTCAAATTCAGCCGAAACTTATCTTCTCGGTGGAGGCTGTT
  GTCTACAATGGCAAGGAGCATGGTCACCTGGAGAAGCTGCAGCGAGTTGTGAAAGGACTGCCTGACCTGC
  AGCGAGTGGTGCTGATCCCCTATGTCCTCCCAAGGGAGAAGATTGACATTTCCAAGATCCCCAACAGTGT
  GTTCCTGGATGATTTCCTGGCAAGCGGGACCGGCGCGCAGGCGCCGCAGCTCGAGTTTGAGCAGCTGCCC
  TTCAGCCACCCTCTGTTCATCATGTTCTCCTCAGGCACGACGGGAGCGCCCAAGTGCATGGTGCACTCAG
  CCGGGGGCACCCTCATCCAGCACCTGAAGGAGCACATGCTACACGGCAACATGACAAGCAGTGACATCCT
  GCTCTACTACACCACGGTCGGCTGGATGATGTGGAACTGGATGGTGTCAGCCCTGGCCACAGGAGCATCC
  TTGGTCCTGTATGATGGCTCCCCGCTGGTTCCGACCCCCAACGTGTTGTGGGACCTTGTGGACAGGATAG
  GGATCACCATCCTGGGAACGGGAGCCAAGTGGCTGTCAGTGCTGGAGGAGAAGGACATGAAGCCAGTGGA
  AACTCACAACCTCCACACGCTGCACACGATCCTGTCCACCGGCTCGCCGCTGAAAGCCCAGAGTTACGAG
  TATGTGTACAGATGCATCAAGAGCTCCGTGCTCCTGGGCTCCATCTCAGGAGGCACTGACATCATCTCCT
  GTTTCATGGGCCAGAACTCCTCTATTCCTGTGTACAAGGGTGAGATCCAAGCCCGGAACCTTGGCATGGC
  TGTGGAAGCCTGGGACGAGGAAGGGAAGGCCGTCTGGGGAGCGAGTGGCGAGCTGGTGTGCACTAAGCCC
  ATTCCCTGCCAGCCCACGCACTTCTGGAACGACGAGAACGGCAGCAAGTACCGGAAGGCTTACTTCTCCA
  AATTCCCAGGTGTCTGGGCACACGGTGACTACTGCAGGATCAACCCCAAAACAGGAGGCATTATCATGCT
  GGGCCGTAGTGATGGCACCCTCAACCCCAATGGCGTCCGCTTTGGCAGCTCGGAGATCTACAACATCGTG
  GAAGCCTTCGATGAGGTGGAGGACAGCCTGTGTGTACCCCAGTACAACAGAGATGGCGAGGAGCGGGTGG
  TCCTGTTCCTGAAGATGGCGTCCGGGCACACTTTCCAGCCTGACCTCGTGAAGCGCATCCGAGACGCCAT
  CCGACTTGGCCTGTCTGCCCGCCATGTGCCCAGCCTCATCCTGGAGACCCGAGGCATTCCATACACACTC
  AATGGCAAGAAAGTGGAGGTGGCCGTGAAGCAGGTGATGGCTGGGAGGACTGTGGAGCACCGGGGGGCCT
  TCTCCAACCCCGAGACCCTCGACCTGTACCGGGACATCCCTGAGCTGCAGGACTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209932 representing NM_030210
  Red=Cloning site Green=Tags(s)
  
  MSKLARLEREEIMECQVMWEPDSKKDTQMDRFRAAVGTACGLALGNYNDLYHWSVRSYMDFWAEFWKFSG
  IVYSRMYDEVVDTSKGIADVPEWFRGSRLNYAENLLRHKENDRVALYVAREGREEIVKVTFEELRQQVAL
  FAAAMRKMGVKKGDRVVGYLPNSAHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAV
  VYNGKEHGHLEKLQRVVKGLPDLQRVVLIPYVLPREKIDISKIPNSVFLDDFLASGTGAQAPQLEFEQLP
  FSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHMLHGNMTSSDILLYYTTVGWMMWNWMVSALATGAS
  LVLYDGSPLVPTPNVLWDLVDRIGITILGTGAKWLSVLEEKDMKPVETHNLHTLHTILSTGSPLKAQSYE
  YVYRCIKSSVLLGSISGGTDIISCFMGQNSSIPVYKGEIQARNLGMAVEAWDEEGKAVWGASGELVCTKP
  IPCQPTHFWNDENGSKYRKAYFSKFPGVWAHGDYCRINPKTGGIIMLGRSDGTLNPNGVRFGSSEIYNIV
  EAFDEVEDSLCVPQYNRDGEERVVLFLKMASGHTFQPDLVKRIRDAIRLGLSARHVPSLILETRGIPYTL
  NGKKVEVAVKQVMAGRTVEHRGAFSNPETLDLYRDIPELQDF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030210
• ORF Size: 2016 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030210.2
• RefSeq Size: 3175 bp
• RefSeq ORF: 2019 bp
• Locus ID: 78894
• UniProt ID: Q9D2R0
• Cytogenetics: 5 G1.1
• MW: 75.7 kDa
• Gene Summary: Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development (By similarity).[UniProtKB/Swiss-Prot Function]