Nuak1 (NM_001004363) Mouse Tagged ORF Clone

CAT#: MR209827

Nuak1 (Myc-DDK-tagged) - Mouse NUAK family, SNF1-like kinase, 1 (Nuak1)

Product Images

Circular map for MR209827

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nuak1
• Synonyms: AU014801; AW494241; B230104P22Rik; Omphk1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209827 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGGGCGGCAGTGTCCGCGGCAGGGGATGGCCCTGCTGTAGAGACCGGCTTGCCGGGCTCTCCGC
  TGGAGGCGGTGGCCGGGGCGACTGCAGCCCCAGTGGAGCCCAGGAAGCCGCACGGGGTGAAGCGACATCA
  CCACAAACACAACTTGAAGCATCGCTATGAGCTGCAAGAGACCCTGGGCAAAGGTACCTACGGCAAAGTC
  AAGAGAGCCACCGAGAGGTTTTCAGGCCGAGTGGTTGCTATAAAATCCATCCGTAAGGACAAAATTAAGG
  ACGAGCTAGACATGGTTCACATCAGACGAGAGATTGAGATCATGTCATCCCTCAACCATCCTCATATCAT
  CAGTATTTATGAAGTGTTTGAGAACAAAGATAAGATTGTGATCATCATGGAATATGCCAGCAAAGGAGAG
  CTGTATGATTACATCAGTGAGCGCAGGCGCCTCAGCGAGAGGGAGACCAGACACTTCTTCCGGCAGATTG
  TGTCTGCCGTGCATTACTGTCACAAGAATGGTGTGGTCCACCGGGACTTGAAGCTGGAGAACATACTGCT
  TGATGACAACTGCAACATTAAGATTGCTGACTTTGGGCTCTCCAACCTGTACCAGAAGGACAAGTTCTTG
  CAAACATTTTGTGGGAGCCCACTCTATGCGTCGCCCGAGATTGTCAACGGCAGGCCTTACCGAGGGCCAG
  AGGTGGACAGCTGGGCCCTGGGTGTCTTGCTTTACACTCTCATTTATGGAACGATGCCCTTCGATGGCTT
  TGATCACAAAAACCTCATCCGGCAGATCAGCAGCGGAGAGTACCGGGAGCCCACACAACCCTCAGATGCT
  CGGGGACTCATCCGGTGGATGCTGATGGTGAATCCCGATCGCAGAGCCACCATCGAGGACATCGCCAACC
  ATTGGTGGGTGAACTGGGGCTACAAGAGCAGCGTCTGTGACTGTGATGCCCTTCCTGACTCTGAGTCTCC
  ACTCTTGGCACGGATCATCGATTGGCATCACCGTTCCACGGGGCTGCAGGCTGAGGCTGAAGCCAAGATG
  AAGGGTCTGGCCAAACCCGGGGCCTCTGAGGTTGTGTTGGAGCGGCAGCGGTCACTGAAGAAGTCCAAGA
  AAGAGAACGACTTTCCCCAGTCTGGCCAGGACTCCGTACCTGAAAGCCCATCCAAGCTGAGTTCCAAGAG
  GCCCAAGGGCATCCTGAAGAAACGGAGCAACAGCGAACACCGCTCCCACAGCACTGGCTTTATCGAAGGC
  ATTGTGAGCCCTGCCTTACCATCCCCTTTCAAAATGGAGCAAGATTTGTGCCGGACTGCCATCCCCCTCC
  CCAGCTCCCCCGAGGCAGATATGTCAGGGAAGCTCAGCCTCAAACAGTCTGCCACGATGCCCAAGAAAGG
  CATCTTGAAAAAGACCCAGCAGAGAGAATCTGGCTACTATTCATCCCCAGAGCGCAGCGAGTCCTCGGAA
  CTGCTGGACAGCAACGATGTGGTCATCAGTGGTGGCCTCTCGTCCCCTCCCCCAGACCCAGCCAGGGGAA
  CTTCCCACAGCCTGTCCTGCCGGAGGAAGGGCATCTTAAAACATAGTAGCAGGTACTCAGATGGTGGCAC
  AGACCCTGCCCTCACCCGCCCTGAAATGCCCACACTGGAATCCTTGTCACCTCCCGGTGTCCCCTCCGAT
  GGCATCTCTCGGAGCTACAGTCGCCCGTCCAGTATCATCAGTGATGACAGTGTCCTGTCCAGCGACTCTT
  TCGACTTGTTAGAGCTTCAAGAGAACCGGCCAGCCCGCCAGCGCATCCGCAGCTGCGTCTCTGCTGAAAA
  CTTCCTCCAGCTTCAGGACTTTGAGACACCACACAACCGGCCCCGGCCCCAGTACCTGAAGCGGCTGGCA
  GACAGTAGCTTCTCCCTTCTCACTGATATGGACGATGTGACTCAGGTGTATAAGAAAGCGCTGGAGATCT
  GCAGCAAGCTCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209827 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEGAAVSAAGDGPAVETGLPGSPLEAVAGATAAPVEPRKPHGVKRHHHKHNLKHRYELQETLGKGTYGKV
  KRATERFSGRVVAIKSIRKDKIKDELDMVHIRREIEIMSSLNHPHIISIYEVFENKDKIVIIMEYASKGE
  LYDYISERRRLSERETRHFFRQIVSAVHYCHKNGVVHRDLKLENILLDDNCNIKIADFGLSNLYQKDKFL
  QTFCGSPLYASPEIVNGRPYRGPEVDSWALGVLLYTLIYGTMPFDGFDHKNLIRQISSGEYREPTQPSDA
  RGLIRWMLMVNPDRRATIEDIANHWWVNWGYKSSVCDCDALPDSESPLLARIIDWHHRSTGLQAEAEAKM
  KGLAKPGASEVVLERQRSLKKSKKENDFPQSGQDSVPESPSKLSSKRPKGILKKRSNSEHRSHSTGFIEG
  IVSPALPSPFKMEQDLCRTAIPLPSSPEADMSGKLSLKQSATMPKKGILKKTQQRESGYYSSPERSESSE
  LLDSNDVVISGGLSSPPPDPARGTSHSLSCRRKGILKHSSRYSDGGTDPALTRPEMPTLESLSPPGVPSD
  GISRSYSRPSSIISDDSVLSSDSFDLLELQENRPARQRIRSCVSAENFLQLQDFETPHNRPRPQYLKRLA
  DSSFSLLTDMDDVTQVYKKALEICSKLN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004363
• ORF Size: 1977 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004363.2
• RefSeq Size: 5032 bp
• RefSeq ORF: 1977 bp
• Locus ID: 77976
• UniProt ID: Q641K5
• Cytogenetics: 10 C1
• MW: 73.7 kDa
• Gene Summary: Serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. Phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1. May be involved in DNA damage response: phosphorylates p53/TP53 at 'Ser-15' and 'Ser-392' and is recruited to the CDKN1A/WAF1 promoter to participate to transcription activation by p53/TP53. May also act as a tumor malignancy-associated factor by promoting tumor invasion and metastasis under regulation and phosphorylation by AKT1. Suppresses Fas-induced apoptosis by mediating phosphorylation of CASP6, thereby suppressing the activation of the caspase and the subsequent cleavage of CFLAR. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with STK11, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair.[UniProtKB/Swiss-Prot Function]