Hspa5 (NM_001163434) Mouse Tagged ORF Clone

CAT#: MR209794

  • TrueORF®

Hspa5 (Myc-DDK-tagged) - Mouse heat shock protein 5 (Hspa5), transcript variant 1

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro


  "NM_001163434" in other vectors (2)


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Reconstitution Protocol

USD 916.00

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Size
    • 10 ug

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Frequently bought together (3)
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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Hspa5
Synonyms AL022860; AU019543; baffled; Bip; D2Wsu17e; D2Wsu141e; Grp78; Hsce70; mBiP; SEZ-7; Sez7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR209794 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGAAGTTCACTGTGGTGGCGGCGGCGTTGCTGCTGCTGGGCGCGGTGCGGGCCGAGGAGGAGGACA
AGAAGGAGGATGTGGGCACGGTGGTCGGCATCGACTTGGGGACCACCTATTCCTGCGTCGGTGTGTTCAA
GAACGGCCGCGTGGAGATCATAGCCAACGATCAGGGCAACCGCATCACGCCGTCGTATGTGGCCTTCACT
CCTGAAGGGGAGCGTCTGATTGGCGATGCGGCCAAGAACCAACTCACGTCCAACCCCGAGAACACGGTCT
TCGATGCCAAGCGCCTCATCGGACGCACTTGGAATGACCCTTCGGTGCAGCAGGACATCAAGTTCTTGCC
ATTCAAGGTGGTTGAAAAGAAAACTAAACCGTACATTCAAGTTGATATTGGAGGTGGGCAAACCAAGACA
TTTGCCCCAGAAGAAATTTCTGCCATGGTTCTCACTAAAATGAAGGAGACTGCTGAGGCGTATTTGGGAA
AGAAGGTTACCCATGCAGTTGTTACTGTACCAGCTTACTTCAATGATGCCCAGCGACAAGCAACCAAAGA
TGCTGGCACTATTGCTGGACTGAATGTCATGAGGATCATCAATGAGCCTACAGCAGCTGCTATTGCATAT
GGCCTGGATAAGAGAGAGGGAGAGAAGAACATCCTTGTGTTTGACCTGGGTGGCGGCACCTTCGATGTGT
CTCTTCTCACCATTGACAATGGTGTCTTTGAAGTGGTGGCCACTAATGGAGATACTCACCTGGGTGGGGA
AGACTTTGATCAGCGGGTCATGGAACACTTCATCAAGTTGTACAAAAAGAAAACTGGTAAAGATGTTAGG
AAAGACAACAGAGCTGTGCAGAAACTCCGGCGTGAGGTAGAAAAGGCTAAGAGAGCCTTGTCTTCTCAGC
ATCAAGCAAGGATTGAAATTGAGTCCTTCTTCGAAGGAGAAGACTTCTCAGAGACCCTTACTCGGGCCAA
ATTTGAAGAGCTGAACATGGACCTGTTCCGCTCTACCATGAAGCCTGTCCAGAAAGTGTTGGAAGACTCT
GATCTGAAGAAATCTGATATTGATGAAATTGTTCTGGTTGGTGGATCTACTCGAATTCCAAAGATTCAGC
AACTGGTGAAAGAGTTCTTCAATGGCAAGGAGCCATCCCGTGGCATAAACCCCGATGAGGCTGTAGCCTA
TGGTGCCGCTGTCCAGGCTGGTGTCCTCTCTGGTGATCAGGATACAGGTGATCTGGTACTGCTTGATGTT
TGTCCCCTTACACTTGGTATTGAAACTGTGGGAGGAGTCATGACAAAACTGATTCCAAGGAACACTGTGG
TACCCACCAAGAAGTCTCAGATCTTCTCCACGGCTTCCGATAATCAGCCAACTGTAACAATCAAGGTCTA
TGAAGGTGAACGACCCCTAACAAAAGACAATCATCTTCTGGGTACATTTGATCTGACTGGAATTCCTCCT
GCTCCCCGTGGAGTTCCCCAGATTGAAGTCACTTTTGAGATAGATGTTAATGGTATTCTCCGAGTGACAG
CTGAAGACAAAGGTACAGGAAACAAAAACAAAATCACAATTACCAATGACCAAAACCGCCTGACACCTGA
AGAAATTGAAAGGATGGTTAATGATGCTGAGAAGTTTGCTGAGGAAGACAAAAAGCTCAAAGAGCGCATT
GACACCAGGAATGAATTGGAAAGCTATGCTTATTCTCTCAAGAACCAGATTGGAGATAAAGAAAAGCTGG
GAGGTAAACTTTCTTCTGAAGATAAAGAAACCATGGAAAAAGCTGTAGAGGAAAAGATTGAATGGCTGGA
AAGCCACCAGGATGCGGACATTGAAGACTTTAAAGCCAAAAAGAAGGAACTAGAAGAAATTGTTCAGCCA
ATTATCAGCAAACTCTATGGAAGTGGAGGCCCTCCCCCAACTGGTGAAGAGGATACATCAGAAAAAGATG
AGTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR209794 protein sequence
Red=Cloning site Green=Tags(s)

MMKFTVVAAALLLLGAVRAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFT
PEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKT
FAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAY
GLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVR
KDNRAVQKLRREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDS
DLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDV
CPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPP
APRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERI
DTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQP
IISKLYGSGGPPPTGEEDTSEKDEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001163434
ORF Size 1968 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001163434.1, NP_001156906.1
RefSeq Size 2534 bp
RefSeq ORF 1968 bp
Locus ID 14828
UniProt ID P20029
Cytogenetics 2 22.94 cM
MW 72.4 kDa
Gene Summary Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (PubMed:12411443, PubMed:12475965). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (PubMed:12411443). Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (By similarity). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (By similarity). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (By similarity). Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating.[UniProtKB/Swiss-Prot Function]

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