Glb1 (NM_009752) Mouse Tagged ORF Clone

CAT#: MR209735

Glb1 (Myc-DDK-tagged) - Mouse galactosidase, beta 1 (Glb1)

Product Images

Circular map for MR209735

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Glb1
• Synonyms: AW125515; Bg; Bge; Bgl; Bgl-; Bgl-e; Bgl-s; Bgl-t; Bgs; Bgt; C130097A14Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209735 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCGGGTCCCCCTGTGTACGCCGCTCCCGCTCCTGGCACTGCTGCAACTGCTGGGCGCTGCGCACG
  GCATCTATAATGTCACCCAGAGGACATTTAAGCTCGACTACAGCCGGGACCGCTTCCTCAAGGATGGACA
  GCCATTCCGATACATCTCGGGAAGCATTCATTACTTCCGGATACCCCGCTTCTACTGGGAGGACCGGCTG
  CTGAAGATGAAGATGGCTGGGCTGAATGCTATCCAGATGTACGTGCCCTGGAACTTCCATGAACCCCAAC
  CAGGACAATATGAGTTTTCTGGGGACCGTGATGTGGAGCATTTCATCCAGCTGGCTCATGAGCTGGGACT
  CCTGGTGATCCTGAGGCCTGGGCCCTACATCTGTGCAGAGTGGGACATGGGGGGCTTACCTGCTTGGCTA
  CTAGAGAAACAATCTATCGTTCTCCGGTCTTCTGACCCAGACTACCTTGTAGCTGTGGATAAATGGCTGG
  CAGTCCTTCTGCCCAAGATGAAGCCCCTGCTCTACCAGAACGGAGGACCGATCATAACCGTGCAGGTTGA
  GAATGAGTACGGGTCCTACTTTGCCTGCGATTACGACTACCTACGCTTCCTGGTGCACCGCTTCCGCTAC
  CATCTGGGTAATGACGTCATTCTCTTCACCACCGACGGAGCAAGTGAAAAAATGCTGAAGTGTGGGACCC
  TGCAGGACCTGTACGCCACAGTGGATTTTGGAACAGGCAACAATATCACACAAGCTTTCCTGGTCCAGAG
  GAAGTTTGAACCTAAAGGACCTTTGATCAATTCTGAGTTCTATACTGGCTGGCTAGACCACTGGGGTAAA
  CCCCATTCCACGGTGAAAACTAAAACACTGGCTACCTCCCTCTATAACCTGCTTGCCCGTGGGGCCAACG
  TGAACTTGTACATGTTTATAGGTGGGACCAATTTTGCCTATTGGAATGGTGCCAACACGCCCTATGAGCC
  ACAGCCCACCAGCTATGACTACGACGCCCCACTGAGCGAGGCTGGGGACCTCACTAAGAAGTATTTTGCT
  CTTCGAGAAGTCATTCAGATGTTTAAAGAAGTCCCAGAAGGCCCTATCCCTCCGTCTACACCCAAATTTG
  CATATGGAAAAGTTGCTCTGAGAAAGTTCAAGACAGTGGCTGAAGCTCTGGGTATCCTGTGTCCCAATGG
  GCCAGTGAAAAGCCTCTATCCCCTGACATTCACTCAGGTAAAACAGTATTTTGGGTATGTGCTGTACCGA
  ACAACGCTTCCTCAAGATTGCAGTAACCCGAAACCCATTTTCTCTTCACCCTTCAATGGCGTCCGTGATC
  GGGCTTACGTCTCTGTGGACGGGGTCCCCCAAGGAATCCTTGATCGAAACCTCATGACAGCTCTGAACAT
  ACAGGGGAAGGCTGGAGCCACGCTGGACATCCTGGTGGAGAACATGGGGCGTGTGAACTATGGCAGATTC
  ATCAATGACTTCAAGGGTTTGATTTCCAACATGACTATCAACTCCACTGTCCTCACCAACTGGACGGTCT
  TCCCACTGGACACTGAGGCCATGGTACGCAACCATCTCTGGGGCCGGGAGGCCAGTGATGGGGGTCACCT
  TGACGGACGGTCGACCTCCAATTCTTCGGACCTCATACTCCCCACCTTTTACGTGGGCAACTTCTCCATC
  CCCTCGGGCATCCCAGACCTGCCACAGGACACCTTCATCCAGTTTCCTGGGTGGTCCAAGGGTCAAGTAT
  GGATCAATGGCTTTAACCTCGGCCGATACTGGCCCACAATGGGCCCACAAAAGACCTTGTTCGTGCCAAG
  GAACATCCTGACCACTTCAGCCCCAAACAACATCACAGTGTTGGAGCTAGAGTTTGCACCCTGCAGCGAG
  GGGACCCCAGAGCTGTGTACAGTAGAGTTTGTTGACACTCCGGTCATTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209735 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLRVPLCTPLPLLALLQLLGAAHGIYNVTQRTFKLDYSRDRFLKDGQPFRYISGSIHYFRIPRFYWEDRL
  LKMKMAGLNAIQMYVPWNFHEPQPGQYEFSGDRDVEHFIQLAHELGLLVILRPGPYICAEWDMGGLPAWL
  LEKQSIVLRSSDPDYLVAVDKWLAVLLPKMKPLLYQNGGPIITVQVENEYGSYFACDYDYLRFLVHRFRY
  HLGNDVILFTTDGASEKMLKCGTLQDLYATVDFGTGNNITQAFLVQRKFEPKGPLINSEFYTGWLDHWGK
  PHSTVKTKTLATSLYNLLARGANVNLYMFIGGTNFAYWNGANTPYEPQPTSYDYDAPLSEAGDLTKKYFA
  LREVIQMFKEVPEGPIPPSTPKFAYGKVALRKFKTVAEALGILCPNGPVKSLYPLTFTQVKQYFGYVLYR
  TTLPQDCSNPKPIFSSPFNGVRDRAYVSVDGVPQGILDRNLMTALNIQGKAGATLDILVENMGRVNYGRF
  INDFKGLISNMTINSTVLTNWTVFPLDTEAMVRNHLWGREASDGGHLDGRSTSNSSDLILPTFYVGNFSI
  PSGIPDLPQDTFIQFPGWSKGQVWINGFNLGRYWPTMGPQKTLFVPRNILTTSAPNNITVLELEFAPCSE
  GTPELCTVEFVDTPVIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009752
• ORF Size: 1944 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009752.1, NM_009752.2, NP_033882.1
• RefSeq Size: 2396 bp
• RefSeq ORF: 1944 bp
• Locus ID: 12091
• UniProt ID: P23780
• Cytogenetics: 9 64.4 cM
• MW: 73 kDa
• Gene Summary: This gene encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The encoded protein is a lysosomal enzyme that catalyzes the hydrolysis of terminal beta-D-galactose residues in various substrates like lactose, ganglioside GM1 and other glycoproteins. Mutations in the human gene are associated with GM1-gangliosidosis and Morquio B syndrome. Disruption of the mouse gene mirrors the symptoms of human gangliosidosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]