Amfr (NM_011787) Mouse Tagged ORF Clone

CAT#: MR209712

Amfr (Myc-DDK-tagged) - Mouse autocrine motility factor receptor (Amfr)

Product Images

Circular map for MR209712

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Amfr
• Synonyms: gp78
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209712 representing NM_011787
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCTGCTCTTCCTCGAGCGCTTCCCGTGGCCCAGCCTCCGCACCTACACGGGCCTCAGCGGCCTGG
  CCTTGCTGGGCACCATCGTCAGCGCCTACCGCGCCCTCAGTCAGCCGGAGGACGGCTCGGGTGAGCCGGA
  GCCGCTAACGGCCCCACTGCAGCCCGAGGCGCGCCTGACCGCCGGCGGCCCCCGGGCCCGCGACGTGGCC
  CAGTACCTGCTGTCGGACAGCCTGTTCGTGTGGGTTCTGGTGAATACTGCTTGCTGTGTTTTGATGTTGG
  TGGCTAAGCTTATCCAGTGTATTGTGTTCGGCCCTCTTCGAGTGAGTGAAAGACAGCATCTCAAAGACAA
  GTTTTGGAATTTTATTTTCTACAAGTTCATTTTCATTTTTGGAGTGCTGAATGTCCAGACTGTGGAGGAA
  GTGGTGATGTGGTGCCTCTGGTTTGCTGGACTTGTCTTCCTGCATCTGATGGTTCAGCTCTGCAAAGACA
  GATTTGAATACCTTTCCTTTTCTCCCACCACTCCAATGAGCAGCCATGGGAGAGTCCTGTCCCTGCTGAT
  TGCTATGCTGCTCTCCTGCTGTGGACTGGCAGTCGTCTGCTGTGTCACAGGCTACACACATGGCATGCAC
  ACCTTGGCTTTCATGGCTGCAGAGTCTCTTCTTGTGACCGTGAGAACAGCTCATGTGATTTTAAGATATG
  TAATTCACCTCTGGGACCTCAACCACGAAGGGACGTGGGAAGGAAAGGGAACCTATGTGTATTACACCGA
  CTTTGTCATGGAGCTCGCTCTCCTGTCCCTGGACCTCATGCACCACATCCACATGCTGTTATTTGGCAAC
  ATCTGGTTATCTATGGCTAGCTTGGTCATCTTTATGCAACTTCGTTACCTGTTCCATGAGGTACAGCGTC
  GAATCCGTCGACACAAGAATTATTTGCGAGTGGTAGGAAACATGGAAGCCAGGTTTGCTGTTGCAACTCC
  AGAGGAACTAGCTGTCAATAATGATGACTGTGCAATCTGCTGGGACTCCATGCAGGCTGCAAGGAAACTG
  CCCTGTGGACATCTTTTTCACAACTCCTGCCTTCGTTCTTGGCTAGAACAAGACACCTCTTGTCCAACAT
  GCAGAATGTCTCTTAATATTGCTGATGGCAGTCGTGCCAGGGAAGACCATCAAGGAGAGAACCTGGACGA
  GAACTTGGTTCCTGTAGCAGCTGCCGAAGGCAGGCCTCGCTTGAACCAGCACAATCACTTCTTCCACTTC
  GATGGGTCCCGGATTGCAAGCTGGCTGCCAAGTTTTTCAGTTGAAGTGATGCACACTACCAACATTCTTG
  GCATCACGCAGGCAAGCAACTCTCAGCTAAATGCCATGGCTCATCAGATTCAGGAGATGTTCCCTCAGGT
  CCCGTACCACCTTGTGCTCCAGGACCTCCAGATGACACGCTCCGTGGAGATAACAACAGATAACATCTTA
  GAAGGACGGATTCAAGTACCTTTCCCCACGCAGCGGTCGGATAGCCTGAGGCCTGCACTGAACAGCCCAG
  TGGAGAGGCCCAGCCCTGACCTGGAGGAGGGAGAAGCTTCTGTCCAGACGGAGCGTGTACCACTGGACCT
  CAGTCCTCGCCTAGAGGAGACCTTGGACTTCAGTGAGGTAGAACTAGAGCCTATTGAGGTGGAAGACTTT
  GAGGCCCGTGGAAGCCGCTTCTCCAAGTCTGCTGATGAGAGACAGCGCATGTTAGTTCAGCGCAAGGATG
  ACCTCCTGCAGCAGGCTCGAAAGCGGTTCTTAAACAAAAGTTCTGAAGATGATGGGGCCTCAGAGAGACT
  CCTCCCTTCTGAAGGCACATCCTCTGACCCCGTGACCCTGCGCCGAAGGATGCTGGCAGCCGCTGCTGAG
  CGGAGACTTCAGAGGCAACGGACAACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209712 representing NM_011787
  Red=Cloning site Green=Tags(s)
  
  MPLLFLERFPWPSLRTYTGLSGLALLGTIVSAYRALSQPEDGSGEPEPLTAPLQPEARLTAGGPRARDVA
  QYLLSDSLFVWVLVNTACCVLMLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEE
  VVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPMSSHGRVLSLLIAMLLSCCGLAVVCCVTGYTHGMH
  TLAFMAAESLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELALLSLDLMHHIHMLLFGN
  IWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKL
  PCGHLFHNSCLRSWLEQDTSCPTCRMSLNIADGSRAREDHQGENLDENLVPVAAAEGRPRLNQHNHFFHF
  DGSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDLQMTRSVEITTDNIL
  EGRIQVPFPTQRSDSLRPALNSPVERPSPDLEEGEASVQTERVPLDLSPRLEETLDFSEVELEPIEVEDF
  EARGSRFSKSADERQRMLVQRKDDLLQQARKRFLNKSSEDDGASERLLPSEGTSSDPVTLRRRMLAAAAE
  RRLQRQRTT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011787
• ORF Size: 1917 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011787.2, NP_035917.2
• RefSeq Size: 3694 bp
• RefSeq ORF: 1920 bp
• Locus ID: 23802
• UniProt ID: Q9R049
• Cytogenetics: 8 45.96 cM
• MW: 73.2 kDa
• Gene Summary: E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also regulates ERAD through the ubiquitination of UBL4A a component of the BAG6/BAT3 complex. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis (By similarity).[UniProtKB/Swiss-Prot Function]