Cul4b (NM_001110142) Mouse Tagged ORF Clone

CAT#: MR209424

Cul4b (Myc-DDK-tagged) - Mouse cullin 4B (Cul4b), transcript variant 2

Product Images

Circular map for MR209424

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cul4b
• Synonyms: 2700050M05Rik; AA409770; CUL-4B; mKIAA0695
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209424 representing NM_001110142
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACGTTCAACTAGGTCTAAAGAAAGAAGAGAGAATGACACCGACTCAGAAGATAACAGTTCGGAAA
  CCTCCAATCAAGAGCGCCGAAGGTGTCGCCAAGGTCCGCCCCGCCCTCCCTATCCACCTCTTTTACCCCC
  CGTGTTCCCTCCCCCAACCCCCCCACCTCAAGTCCGGCGGACCCGGGGGCTTCAGGATCTGGGAGCTATG
  AAAAGTGTCTGCCCAGGCACTTCCGGTTTTTCTTCCCCCAATCCCTCTGCTGCTTCTGCTGCTGCTCAGG
  AGGTCAGATCTGCCACTGATGGTAATACCAGCACCACTCCGCCCACCTCTGCCAAGAAGAGAAAGTTAAA
  CAGCAGCAGTAGCAGCAGTAACAGTAGTAACGAGAGAGAAGACTTTGATTCCACCTCTTCCTCTTCCACC
  CCTCCACAACCCAGAGATTCGGCGTCCCCTTCAACCTCGTCCTTCTGCCTTGGGGTTCCAGTGGCCACTT
  CCAGCCACGTACCTATACAGAAGAAGCTGCGGTTTGAAGACACCCTGGAGTTTGTAGGGATTGATACGAA
  AATGGCTGAGGAATCTTCCTCCTCATCTTCCTCATCTTCGCCAACTGCTGCAACCTCACAGCAGCAGCAG
  CAGCAACAACTTAAAACTAAGAGTATATTAATTTCTTCTGTGGCTTCAGTGCACCATGCAAACGGCCTGG
  CTAAATCTTCTACCGCGGTCTCTAGCTTTGCTAACAGCAAGCCCGGCTCAGCTAAGAAGTTAGTGATCAA
  GAACTTTAAAGATAAGCCTAAATTACCAGAAAACTATACGGATGAGACATGGCAAAAGCTAAAAGAAGCA
  GTGGAAGCCATTCAGAATAGTACTTCAATTAAGTACAATTTAGAAGAACTCTACCAGGCTGTAGAAAATC
  TTTGTTCTCATAAGATTTCTGCAAATTTGTATAAGCAGCTGAGGCAGATATGTGAAGATCACATCAAAGC
  ACAGATTCATCAGTTCAGAGAGGATTCATTGGATAGTGTTCTTTTTCTCAAAAAGATCGATAGATGCTGG
  CAAAACCACTGTAGGCAAATGATAATGATCAGGAGCATTTTTTTGTTTCTGGATAGAACTTATGTTCTTC
  AGAATTCAATGCTACCCTCCATTTGGGACATGGGATTAGAGTTATTTAGGGCTCATATTATAAGTGATCA
  AAAAGTACAGACAAAAACGATTGATGGCATCCTTCTCTTGATTGAGAGAGAGCGGAATGGTGAAGCAATT
  GATAGAAGTTTACTTCGAAGCCTTTTAAGTATGCTGTCTGATTTGCAAATTTACCAAGATTCTTTTGAAC
  AGCAATTTTTGCAAGAAACTAATCGGCTTTACGCAGCCGAAGGTCAGAAGTTAATGCAAGAAAGAGAGGT
  TCCTGAGTATCTTCATCATGTTAATAAACGCCTAGAAGAAGAAGCTGACAGACTTATTACTTACTTAGAT
  CAGACCACCCAGAAGTCACTAATTGCTTCTGTTGAAAAACAACTTCTAGGTGAACACTTAACGGCAATTC
  TTCAAAAAGGTTTAAATAGCCTCCTTGATGAAAACCGAATTCAAGATTTGTCTCTCCTGTATCAGCTCTT
  CAGTAGAGTTCGGGGTGGAGTTCAGGTTCTCTTACAGCAGTGGATTGAGTATATCAAGGCTTTTGGAAGT
  ACCATTGTTATTAATCCTGAAAAAGATAAAACTATGGTTCAAGAATTATTGGACTTTAAAGATAAAGTTG
  ACCATATAATTGATACCTGCTTTCTGAAAAATGAAAAATTTATCAATGCCATGAAAGAAGCTTTTGAAAC
  ATTCATTAATAAAAGACCAAATAAGCCAGCTGAACTGATAGCTAAGTATGTGGATTCAAAACTTCGTGCA
  GGCAACAAAGAAGCTACAGATGAAGAGCTTGAGAAAATGTTGGATAAAATAATGATTATATTTAGATTTA
  TCTATGGTAAAGATGTTTTTGAGGCCTTCTATAAGAAAGATTTAGCCAAACGCCTATTAGTTGGCAAGAG
  TGCATCAGTAGATGCTGAAAAATCAATGCTGTCCAAACTGAAACACGAATGTGGAGCCGCTTTTACCAGC
  AAGCTTGAAGGAATGTTCAAAGACATGGAGCTTTCTAAAGATATCATGATTCAGTTTAAACAGTATATGC
  AGAACCAGAATGTACCTGGCAATATTGAATTAACTGTTAATATCCTGACAATGGGTTACTGGCCAACATA
  TGTGCCTATGGAAGTCCATTTGCCGCCAGAGATGGTAAAGCTTCAGGAAATTTTCAAGACATTTTACTTA
  GGCAAGCACAGTGGCAGGAAACTCCAGTGGCAGTCAACCTTGGGACACTGTGTGCTAAAAGCTGAATTTA
  AAGAGGGCAAAAAAGAACTTCAGGTCTCTCTTTTTCAAACCATGGTGCTGCTAATGTTTAATGAAGGAGA
  AGAATTCAGTTTAGAAGAGATCAAGCATGCTACTGGGATAGAGGATGGAGAATTAAGAAGAACACTGCAG
  TCACTAGCCTGTGGCAAAGCTAGAGTTCTGGCTAAAAATCCTAAGGGCAAAGATATTGAAGATGGTGACA
  AATTTATTTGTAATGACGATTTCAAACACAAACTATTCAGGATAAAGATCAATCAAATCCAGATGAAAGA
  AACGGTTGAAGAACAAGCAAGCACTACAGAAAGAGTATTTCAAGACAGACAGTACCAAATTGATGCTGCG
  ATCGTTCGAATTATGAAGATGAGAAAAACACTTAGCCACAATCTTCTTGTTTCAGAAGTCTACAATCAGC
  TAAAATTTCCAGTTAAACCTGCTGATCTTAAGAAGAGAATAGAATCCCTCATTGACCGGGACTACATGGA
  AAGAGATAAAGAAAATCCAAATCAGTACAACTATATTGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209424 representing NM_001110142
  Red=Cloning site Green=Tags(s)
  
  MSRSTRSKERRENDTDSEDNSSETSNQERRRCRQGPPRPPYPPLLPPVFPPPTPPPQVRRTRGLQDLGAM
  KSVCPGTSGFSSPNPSAASAAAQEVRSATDGNTSTTPPTSAKKRKLNSSSSSSNSSNEREDFDSTSSSST
  PPQPRDSASPSTSSFCLGVPVATSSHVPIQKKLRFEDTLEFVGIDTKMAEESSSSSSSSSPTAATSQQQQ
  QQQLKTKSILISSVASVHHANGLAKSSTAVSSFANSKPGSAKKLVIKNFKDKPKLPENYTDETWQKLKEA
  VEAIQNSTSIKYNLEELYQAVENLCSHKISANLYKQLRQICEDHIKAQIHQFREDSLDSVLFLKKIDRCW
  QNHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRAHIISDQKVQTKTIDGILLLIERERNGEAI
  DRSLLRSLLSMLSDLQIYQDSFEQQFLQETNRLYAAEGQKLMQEREVPEYLHHVNKRLEEEADRLITYLD
  QTTQKSLIASVEKQLLGEHLTAILQKGLNSLLDENRIQDLSLLYQLFSRVRGGVQVLLQQWIEYIKAFGS
  TIVINPEKDKTMVQELLDFKDKVDHIIDTCFLKNEKFINAMKEAFETFINKRPNKPAELIAKYVDSKLRA
  GNKEATDEELEKMLDKIMIIFRFIYGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTS
  KLEGMFKDMELSKDIMIQFKQYMQNQNVPGNIELTVNILTMGYWPTYVPMEVHLPPEMVKLQEIFKTFYL
  GKHSGRKLQWQSTLGHCVLKAEFKEGKKELQVSLFQTMVLLMFNEGEEFSLEEIKHATGIEDGELRRTLQ
  SLACGKARVLAKNPKGKDIEDGDKFICNDDFKHKLFRIKINQIQMKETVEEQASTTERVFQDRQYQIDAA
  IVRIMKMRKTLSHNLLVSEVYNQLKFPVKPADLKKRIESLIDRDYMERDKENPNQYNYIA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001110142
• ORF Size: 2910 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110142.1, NP_001103612.1
• RefSeq Size: 5079 bp
• RefSeq ORF: 2913 bp
• Locus ID: 72584
• UniProt ID: A2A432
• Cytogenetics: X A3.3
• MW: 111.1 kDa
• Gene Summary: Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8 (By similarity). With CUL4A, contributes to ribosome biogenesis (By similarity).[UniProtKB/Swiss-Prot Function]