Nr4a1 (NM_010444) Mouse Tagged ORF Clone

CAT#: MR209316

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  • TrueORF®

Nr4a1 (Myc-DDK-tagged) - Mouse nuclear receptor subfamily 4, group A, member 1 (Nr4a1)



  "NM_010444" in other vectors (6)

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Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 544.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Nr4a1
Synonyms Gfrp; GFRP1; Hbr-1; Hbr1; Hmr; N10; NGFI-B; NGFIB; NP10; nur77; TIS1; TR3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR209316 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCTGTATTCAAGCTCAATATGGAACACCAGCAACGAGCCCAGGACCGCGTGACCACCTGACCGGTG
ATCCCCTGGCCCTTGAGTTCGGCAAGCCTACCATGGACCTGGCCAGCCCCGAGACAGCACCTGCCGCACC
TGCTACACTGCCCAGCTTCAGCACCTTCATGGACGGGTACACCGGAGAGTTTGACACCTTCCTCTACCAG
CTGCCGGGGACGACCCAGCCGTGCTCCTCAGCTTGTTCCTCTGCCTCCTCCACGTCTTCTTCCTCATCCT
CGGCCACCTCCCCCGCTTCGGCGTCCTTCAAGTTTGAGGACTTCCAGGTGTACGGCTGCTACCCGGGCAC
CCTGAGCGGCCCATTAGATGAGACCCTATCCTCCAGCGGCTCTGAGTACTATGGCAGTCCCTGCTCAGCC
CCCTCGCCATCTACACCCAACTTCCAGCCGTCCCAGCTTTCTCCCTGGGACGGCTCATTTGGCCACTTCT
CCCCGAGCCAGACTTATGAAGGCCTCTGGGCATGGACAGAGCAGTTGCCTAAGGCTTCTTCAGGGCCTCC
GCCACCTCCAACCTTCTTCTCCTTCAGTCCTCCCACTGGCCCCAGCCCCAGCCTGGCCCAGAGTTCTCTG
AAATTGTTCCCACCACCAGCCACCCACCAGCTTGGGGAGGGGGAGAGCTATTCCATGCCAGCAGCTTTCC
CCGGCTTGGCACCCACCTCTCCGAACCGTGACACTTCCGGCATTCTGGACGCACCCGTGACCTCCACCAA
GTCCCGGAGCGGGGCTTCAGGTGGCAGCGAGGGCCGCTGTGCAGTCTGTGGTGACAATGCTTCGTGTCAG
CACTATGGGGTCCGCACCTGTGAGGGCTGCAAGGGCTTCTTCAAGCGCACAGTACAGAAAAGCGCCAAGT
ACATCTGCCTGGCAAACAAGGATTGCCCTGTGGACAAGAGGCGGCGGAACCGCTGCCAGTTCTGCCGCTT
CCAGAAGTGCCTGGCTGTGGGCATGGTGAAGGAAGTTGTACGGACAGACAGCCTAAAAGGGCGGCGGGGC
CGGCTACCTTCAAAACCCAAGCAGCCTCCAGATGCCTCCCCTACCAATCTTCTCACTTCCCTCATCCGGG
CACACTTGGACTCCGGGCCTAGCACTGCCAAATTGGACTATTCCAAGTTCCAGGAACTGGTGCTGCCCCG
CTTCGGGAAGGAAGATGCCGGTGACGTGCAACAATTTTATGACTTGCTCTCTGGTTCCCTGGACGTTATC
CGAAAGTGGGCAGAAAAAATCCCTGGCTTCATTGAGCTTTGCCCAGGAGACCAAGACCTGTTGCTAGAGT
CTGCCTTCCTGGAACTCTTCATCCTCCGCCTGGCATACCGATCTAAACCCGGTGAGGGGAAGCTCATCTT
CTGCTCAGGCCTGGTACTACACCAGCTGCAGTGTGCCCGTGGCTTTGGTGATTGGATTGACAACATCCTG
GCCTTCTCACGGTCCCTGCACAGCTTGGGTGTTGATGTTCCCGCCTTTGCCTGCCTGTCCGCTCTGGTCC
TCATCACTGATCGACACGGGCTCCAGGACCCTCGTCGGGTGGAAGAGCTGCAGAATCGCATTGCTAGCTG
TCTGAAGGAGCACATGGCTACCGTGGCAGGAGACCCACAGCCGGCCAGCTGCCTGTCACGTCTGCTGGGC
AAACTGCCTGAGCTTCGGACCCTGTGCACTCAAGGCCTGCAGCGCATCTTTTGCCTCAAGTTGGAGGACT
TGGTACCCCCTCCACCTATTGTGGACAAGATCTTTATGGACACATTGTCTTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR209316 protein sequence
Red=Cloning site Green=Tags(s)

MPCIQAQYGTPATSPGPRDHLTGDPLALEFGKPTMDLASPETAPAAPATLPSFSTFMDGYTGEFDTFLYQ
LPGTTQPCSSACSSASSTSSSSSSATSPASASFKFEDFQVYGCYPGTLSGPLDETLSSSGSEYYGSPCSA
PSPSTPNFQPSQLSPWDGSFGHFSPSQTYEGLWAWTEQLPKASSGPPPPPTFFSFSPPTGPSPSLAQSSL
KLFPPPATHQLGEGESYSMPAAFPGLAPTSPNRDTSGILDAPVTSTKSRSGASGGSEGRCAVCGDNASCQ
HYGVRTCEGCKGFFKRTVQKSAKYICLANKDCPVDKRRRNRCQFCRFQKCLAVGMVKEVVRTDSLKGRRG
RLPSKPKQPPDASPTNLLTSLIRAHLDSGPSTAKLDYSKFQELVLPRFGKEDAGDVQQFYDLLSGSLDVI
RKWAEKIPGFIELCPGDQDLLLESAFLELFILRLAYRSKPGEGKLIFCSGLVLHQLQCARGFGDWIDNIL
AFSRSLHSLGVDVPAFACLSALVLITDRHGLQDPRRVEELQNRIASCLKEHMATVAGDPQPASCLSRLLG
KLPELRTLCTQGLQRIFCLKLEDLVPPPPIVDKIFMDTLSF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_010444
ORF Size 1806 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_010444.2
RefSeq Size 2534 bp
RefSeq ORF 1806 bp
Locus ID 15370
UniProt ID P12813
Cytogenetics 15 F1
MW 64.7 kDa
Gene Summary Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation (By similarity). Plays a role in the vascular response to injury (PubMed:22427340).[UniProtKB/Swiss-Prot Function]

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