Pdlim5 (NM_019808) Mouse Tagged ORF Clone

CAT#: MR209208

Pdlim5 (Myc-DDK-tagged) - Mouse PDZ and LIM domain 5 (Pdlim5), transcript variant 1

Product Images

Circular map for MR209208

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pdlim5
• Synonyms: 1110001A05Rik; AI987914; C87059; Enh; Enh1; Enh2; Enh3; LIM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209208 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAACTACAGTGTGTCATTGGTCGGCCCAGCTCCTTGGGGTTTCCGGCTCCAAGGTGGCAAGGATT
  TCAACATGCCTCTGACAATCTCTAGTCTGAAGGATGGTGGCAAGGCATCTCAGGCACATGTCAGAATAGG
  CGACGTGGTTCTCAGCATCGATGGGATCAGTGCACAGGGAATGACGCATCTTGAAGCCCAGAACAAGATT
  AAGGCTTGTATGGGCTCCTTGAATATGACTCTACAAAGAGCTTCAGCTGCAGCCAAGAGTGAGCCGGTTT
  CCGTCCAGAAGGGTGAACCTAAGGAAGTAGTTAAGCCTGTGCCCATTACATCTCCTGCTGTATCCAAAGT
  CACTTCCACTACCAACATGGCCTACAATAAAGCACCACGGCCCTTTGGTTCTGTGTCTTCACCAAAAGTC
  ACATCCATCCCATCACCATCGTCTGCGTTCACCCCAGCCCATGCCGCCACTTCATCACATGCTTCCCCCA
  CACCTGTGGCCGCTGCCACTCCCCTCCACCTCTCTGCATCCGGACTGCATGTTAGTGCCAATCTTAGTGC
  TGACCAGTGTTCATCTCCACCGAACACTGGTAAACCTGCAGTTAATGTCCCACGGCAGCCCACAGTCACC
  AGCGTGTGTTCCGAGTCTGCTCAGGAGCTAGCAGAGGGACAGAGAAGAGGATCCCAGGGTGACATTAAGC
  AGCAAAATGGCCCACCAAGAAAACACATTGTGGAGCGCAACACGGAGTTTTATCACATACCCACTCACAG
  TGATGCCAGCAAGAAACGGCTGATTGAGGACACTGAGGACTGGCGCCCCCGGACTGGAACGACTCAGTCT
  CGTTCTTTCCGGATCCTTGCCCAGATCACTGGGACTGAGCATCTGACAGAATCTGAAAATGACAATACGA
  AGAAGGCAAATAGCACCCAGGAGCCTTCTCAGCAACCGGCGTCATCTGGAGCTTCACCTCTGAGCGCGTC
  TGAGGGCCCCGAGAGCCCAGGTTCCAGCAGGCCCTCGGTGGCTGGCCTCCGCTCCGCTGCTGCTTTCAAG
  CCTGTAGGATCCACCAGCGTCAAGTCACCTAGCTGGCAGCGTCCAAACCAAGCAGCACCTTCTACTGGGA
  GAATTTCAAACAATGCACGTTCTTCAGGTACAGGGGCGTCTGTGGGGCCTCCCCAGCCAAGTGACCAAGA
  CACACTTGTGCAGAGAGCGGAACACATTCCAGCGGGCAAGCGGACCCCCATGTGTGCCCACTGCAACCAA
  GTTATCAGGGGACCTTTTCTTGTGGCTCTGGGGAAGTCCTGGCATCCAGAAGAATTTAACTGTGCTCATT
  GCAAAAACACAATGGCCTACATAGGCTTTGTAGAGGAGAAGGGAGCACTGTACTGTGAGCTATGCTATGA
  GAAGTTCTTTGCTCCTGAATGTGGCCGGTGCCAAAGGAAGATCCTTGGAGAAGTCATCAATGCTTTGAAG
  CAGACCTGGCATGTGTCCTGTTTTGTGTGTGTGGCCTGTGGAAAACCCATTCGTAATAATGTTTTCCACT
  TGGAAGATGGCGAGCCCTACTGTGAGACAGACTACTACGCCCTTTTTGGAACAATATGTCGTGGATGTGA
  ATTCCCCATAGAGGCTGGTGACATGTTCCTGGAAGCTCTGGGATACACTTGGCATGATACTTGCTTTGTG
  TGCTCTGTGTGTTGTGAAAGTTTGGAAGGGCAGACATTTTTCTCCAAGAAGGACAAGCCACTTTGCAAAA
  AACATGCTCATTCTGTGAATTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209208 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSNYSVSLVGPAPWGFRLQGGKDFNMPLTISSLKDGGKASQAHVRIGDVVLSIDGISAQGMTHLEAQNKI
  KACMGSLNMTLQRASAAAKSEPVSVQKGEPKEVVKPVPITSPAVSKVTSTTNMAYNKAPRPFGSVSSPKV
  TSIPSPSSAFTPAHAATSSHASPTPVAAATPLHLSASGLHVSANLSADQCSSPPNTGKPAVNVPRQPTVT
  SVCSESAQELAEGQRRGSQGDIKQQNGPPRKHIVERNTEFYHIPTHSDASKKRLIEDTEDWRPRTGTTQS
  RSFRILAQITGTEHLTESENDNTKKANSTQEPSQQPASSGASPLSASEGPESPGSSRPSVAGLRSAAAFK
  PVGSTSVKSPSWQRPNQAAPSTGRISNNARSSGTGASVGPPQPSDQDTLVQRAEHIPAGKRTPMCAHCNQ
  VIRGPFLVALGKSWHPEEFNCAHCKNTMAYIGFVEEKGALYCELCYEKFFAPECGRCQRKILGEVINALK
  QTWHVSCFVCVACGKPIRNNVFHLEDGEPYCETDYYALFGTICRGCEFPIEAGDMFLEALGYTWHDTCFV
  CSVCCESLEGQTFFSKKDKPLCKKHAHSVNF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019808
• ORF Size: 1776 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019808.1, NM_019808.2, NM_019808.3, NP_062782.2
• RefSeq Size: 5039 bp
• RefSeq ORF: 1776 bp
• Locus ID: 56376
• UniProt ID: Q8CI51
• Cytogenetics: 3 H1
• MW: 63.3 kDa
• Gene Summary: May play an important role in the heart development by scaffolding PKC to the Z-disk region. Isoform 2 and isoform 3 may negatively modulate the scaffolding activity of isoform 1. May play a role in the regulation of cardiomyocyte expansion. Overexpression promotes the development of heart hypertrophy. Contributes to the regulation of dendritic spine morphogenesis in neurons. May restrain postsynaptic growth of excitatory synapses (By similarity).[UniProtKB/Swiss-Prot Function]