Senp2 (NM_029457) Mouse Tagged ORF Clone

SKU MR209170

Senp2 (Myc-DDK-tagged) - Mouse SUMO/sentrin specific peptidase 2 (Senp2), transcript variant 1

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Senp2
• Synonyms: 2310007L05Rik; 4930538C18Rik; AI646780; AW554757; mKIAA1331; Smt3ip2; SuPr-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR209170 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTACAGATGGCTGGCTAAGGTTCTCGGCACCATTCTTCGCTTGTGTGAGCGGCCCGCGCCCGGGGCCC; GCGCCCTACTCAAGAGGCGGCGCTCCAGCAGCACTCTGTTTTCTACTGCAGTGGACACTGATGAGATCCC; AGCCAAAAGGCCAAGATTAGATTGCTTTATTCACCAAGTGAAGAACAGTCTCTACAATGCTGCCAGTTTA; TTTGGATTCCCGTTCCAGCTGACCACAAAACCCATGGTGTCTTCTGCTTGTAATGGAACACGGAATGTGG; CCCCTTCTGGAGAGGTCTTTTCGAACTCTTCATCTTGTGAGCTGATGAGTTCTGGATCCTGCAGCAGCAT; GCTGAAACTGGGTAATAAATCTCCTAATGGAATAAGCGACTATCCAAAGATCAGAGTGACAGTGACCCGC; GATCAGCCACGGAGAGTCCTGCCCTCCTTTGGTTTTACGCTGAAATCGGAAGGCTATAATAGAAGGCCAA; GTGGTCGTCGCCATAGCAAAAGCAATCCAGAGAGTTCCTTAACATGGAAGCCTCAGGAGCAAGGTGTGAC; AGAGATGATTTCTGAAGAGGGTGGCAAGGGTGTGAGGCGTCCCCACTGCACCGTGGAGGAGGGTGTCCAA; AAGGATGAAAGAGAAAAGTATCGAAAGTTATTGGAGCGACTTAAAGAAGGTGCTCATGGAAGCACGTTCC; CTCCCACGGTTTCACACCACAGTTCTCAGAGAATTCAGATGGACACATTAAAGACCAAAGGCTGGGTGGA; GGAGCAGAATCATGGCGTCAGAACAACCCATTTTGTTCCAAAACAGTATAGAGTTGTTGAAACAAGAGGA; CCTCTCTGTTCAATGAGAAGTGAAAAGAGGTATTCAAAAGGGAAAGCTGATACAGAGAAGGTGGTGGGAC; TCCGATTTGAAAAGGAAGGTACAAGAGGACACCAAATGGAGCCTGATCTGTCAGAAGAAGTGTCTGCCCG; ACTTCGTCTAGGAAGTGGAAGCAATGGCTTACTCAGAAGGAAAATATCAGTGCTTGAGATAAAGGAAAAG; AACTTCCCGAGCAAAGAGAAGGACAGAAGAACGGAAGACCTCTTTGAATTTACAGAGGACATGGAAAAGG; AAATCAGTAATGCCCTGGGCCACGGCCCACCAGATGAGATTTTGAGCAGCGCTTTCAAGTTGCGAATCAC; CCGCGGAGACATCCAGACCTTAAAGAACTATCATTGGCTCAACGATGAGGTCATTAATTTTTATATGAAT; CTTTTAGTGGAAAGGAGTAAAAAGCAAGGTTATCCAGCACTTCATGCATTCAGTACATTCTTCTATCCTA; AGCTAAAGTCTGGAGGTTACCAAGCAGTGAAAAGATGGACCAAAGGGGTAAATCTCTTCGAACAAGAACT; TGTTCTGGTACCTATTCATCGGAAGGTACATTGGAGCCTGGTGGTGATGGACCTAAGAAAAAAGTGTCTT; AAATATCTGGATTCTATGGGACAAAAAGGCCACAGGATCTGTGAGATTCTTCTTCAGTATTTACAGGATG; AAAGCAAGACCAAAAGAAATACAGATCTCAACCTCTTAGAGTGGACCCACTACAGCATGAAGCCACATGA; GATTCCTCAGCAGTTGAATGGGAGTGACTGTGGAATGTTTACTTGTAAATATGCAGATTATATTTCTAGG; GACAAACCTATCACATTCACTCAGCACCAGATGCCTCTCTTCCGGAAGAAGATGGTGTGGGAGATCCTTC; ACCAGCAGTTGCTG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR209170 protein sequence; Red=Cloning site Green=Tags(s); ; MYRWLAKVLGTILRLCERPAPGARALLKRRRSSSTLFSTAVDTDEIPAKRPRLDCFIHQVKNSLYNAASL; FGFPFQLTTKPMVSSACNGTRNVAPSGEVFSNSSSCELMSSGSCSSMLKLGNKSPNGISDYPKIRVTVTR; DQPRRVLPSFGFTLKSEGYNRRPSGRRHSKSNPESSLTWKPQEQGVTEMISEEGGKGVRRPHCTVEEGVQ; KDEREKYRKLLERLKEGAHGSTFPPTVSHHSSQRIQMDTLKTKGWVEEQNHGVRTTHFVPKQYRVVETRG; PLCSMRSEKRYSKGKADTEKVVGLRFEKEGTRGHQMEPDLSEEVSARLRLGSGSNGLLRRKISVLEIKEK; NFPSKEKDRRTEDLFEFTEDMEKEISNALGHGPPDEILSSAFKLRITRGDIQTLKNYHWLNDEVINFYMN; LLVERSKKQGYPALHAFSTFFYPKLKSGGYQAVKRWTKGVNLFEQELVLVPIHRKVHWSLVVMDLRKKCL; KYLDSMGQKGHRICEILLQYLQDESKTKRNTDLNLLEWTHYSMKPHEIPQQLNGSDCGMFTCKYADYISR; DKPITFTQHQMPLFRKKMVWEILHQQLL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029457
• ORF Size: 1764 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_029457.3, NP_083733.1
• RefSeq Size: 4499 bp
• RefSeq ORF: 1767 bp
• Locus ID: 75826
• UniProt ID: Q91ZX6
• Cytogenetics: 16 B1
• MW: 67.6 kDa
• Summary: Protease that catalyzes two essential functions in the SUMO pathway. The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway. Deconjugates SUMO2 from MTA1 (By similarity). Plays a dynamic role in adipogenesis by desumoylating and promoting the stabilization of CEBPB (PubMed:20194620). Isoform 3 activates transcription.[UniProtKB/Swiss-Prot Function]