Glud1 (NM_008133) Mouse Tagged ORF Clone

CAT#: MR208853

Glud1 (Myc-DDK-tagged) - Mouse glutamate dehydrogenase 1 (Glud1), nuclear gene encoding mitochondrial protein

Product Images

Circular map for MR208853

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Glud1
• Synonyms: AI118167; Gdh-X; Glud; Gludl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208853 representing NM_008133
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCGCCGTCTGGGCGAAGCGCTGCTGCTGTCCCGCGCCGGGCCCGCTGCCCTGGGCTCTGCGGCTG
  CAGACTCAGCCGCGCTGCTGGGCTGGGCCCGCGGACAGCCCTCCGCCGCCCCGCAACCCGGGCTCACGCC
  GGTCGCCCGGCGCCACTACAGCGAAGCGGCCGCCGACCGCGAAGACGACCCCAACTTCTTCAAGATGGTG
  GAGGGCTTCTTCGACCGCGGCGCCAGCATCGTAGAGGACAAGCTGGTGGAAGACCTGAAGACCCGGGAGA
  GCGAGGAGCAGAAGCGGAACCGGGTGCGCGGCATCCTGAGGATCATCAAGCCCTGCAACCATGTGTTGAG
  CCTCTCCTTCCCCATCCGGCGCGACGACGGCTCCTGGGAGGTCATCGAAGGCTACCGGGCCCAGCACAGC
  CAGCACCGCACGCCCTGCAAGGGAGGTATCCGTTACAGCACTGACGTGAGTGTGGATGAAGTAAAAGCAC
  TGGCTTCCTTAATGACATACAAGTGCGCTGTGGTCGATGTACCGTTTGGAGGTGCTAAAGCAGGCGTTAA
  GATCAACCCCAAGAACTATACAGATAATGAATTAGAAAAAATTACACGGAGGTTCACTATGGAGCTGGCC
  AAGAAGGGTTTTATTGGTCCTGGCATTGATGTGCCTGCCCCAGACATGAGCACGGGTGAGCGGGAGATGT
  CCTGGATCGCTGACACCTATGCCAGCACCATAGGGCACTATGATATCAATGCGCATGCCTGTGTTACTGG
  GAAACCCATCAGTCAAGGAGGCATCCACGGGCGCATCTCCGCTACTGGCCGGGGTGTCTTCCATGGAATT
  GAAAACTTCATCAATGAGGCTTCTTACATGAGCATTTTAGGAATGACACCAGGCTTTGGCGATAAGACAT
  TTGTTGTTCAGGGATTTGGTAATGTGGGCCTGCACTCTATGAGATATTTACATCGTTTTGGTGCTAAATG
  TGTTGGTGTTGGAGAGTCTGATGGGAGTATATGGAATCCGGATGGGATTGACCCAAAAGAACTGGAAGAC
  TTCAAGTTGCAACATGGATCAATTCTGGGCTTCCCCAAAGCCAAGGTCTATGAAGGAAGCATCTTGGAGG
  CTGACTGTGACATTCTGATTCCTGCCGCCAGCGAGAAGCAGTTGACCAAATCCAATGCACCCAGAGTCAA
  AGCCAAGATCATTGCTGAAGGTGCCAATGGGCCAACCACTCCAGAGGCTGATAAGATTTTCCTGGAAAGA
  AACATCATGGTTATTCCCGATCTCTACTTAAATGCTGGAGGAGTAACAGTGTCTTACTTTGAGTGGCTAA
  AGAATCTAAATCATGTCAGCTACGGCCGATTGACCTTCAAATATGAAAGGGACTCTAACTACCACTTGCT
  CATGTCTGTTCAAGAGAGTTTAGAGAGAAAGTTTGGAAAGCATGGTGGAACTATTCCTGTGGTCCCCACA
  GCAGAGTTCCAGGACAGGATATCGGGTGCATCTGAGAAAGACATTGTGCACTCTGGCTTGGCCTACACAA
  TGGAGAGATCTGCCAGGCAAATTATGCGCACAGCCATGAAGTATAACCTGGGATTGGACCTGAGAACAGC
  TGCCTATGTCAATGCTATCGAGAAAGTCTTCAAGGTGTACAATGAAGCTGGTGTGACCTTCACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208853 representing NM_008133
  Red=Cloning site Green=Tags(s)
  
  MYRRLGEALLLSRAGPAALGSAAADSAALLGWARGQPSAAPQPGLTPVARRHYSEAAADREDDPNFFKMV
  EGFFDRGASIVEDKLVEDLKTRESEEQKRNRVRGILRIIKPCNHVLSLSFPIRRDDGSWEVIEGYRAQHS
  QHRTPCKGGIRYSTDVSVDEVKALASLMTYKCAVVDVPFGGAKAGVKINPKNYTDNELEKITRRFTMELA
  KKGFIGPGIDVPAPDMSTGEREMSWIADTYASTIGHYDINAHACVTGKPISQGGIHGRISATGRGVFHGI
  ENFINEASYMSILGMTPGFGDKTFVVQGFGNVGLHSMRYLHRFGAKCVGVGESDGSIWNPDGIDPKELED
  FKLQHGSILGFPKAKVYEGSILEADCDILIPAASEKQLTKSNAPRVKAKIIAEGANGPTTPEADKIFLER
  NIMVIPDLYLNAGGVTVSYFEWLKNLNHVSYGRLTFKYERDSNYHLLMSVQESLERKFGKHGGTIPVVPT
  AEFQDRISGASEKDIVHSGLAYTMERSARQIMRTAMKYNLGLDLRTAAYVNAIEKVFKVYNEAGVTFT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008133
• ORF Size: 1674 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008133.4, NP_032159.1
• RefSeq Size: 3158 bp
• RefSeq ORF: 1677 bp
• Locus ID: 14661
• UniProt ID: P26443
• Cytogenetics: 14 20.8 cM
• MW: 61.8 kDa
• Gene Summary: Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate.[UniProtKB/Swiss-Prot Function]