Aaas (NM_153416) Mouse Tagged ORF Clone

CAT#: MR208691

Aaas (Myc-DDK-tagged) - Mouse achalasia, adrenocortical insufficiency, alacrimia (Aaas)

Product Images

Circular map for MR208691

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aaas
• Synonyms: AAA; ADRACALA; D030041N15Rik; GL003
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208691 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCTCCCTGGGGTTGTTCCCCCCGCCGCCGCCTAGAGGTCAAGTCACCCTGTATGAGCACAATAACG
  AGCTGGTGACCGGCAACAGCTATGAGAGCCCGCCTCCCGACTTTCGGGGCCAGTGGATCAATCTTCCTGT
  CCTACACCTGACCAAGGATCCCCTAAAAGCCCCAGGGAGGCTGGACCATGGTACAAGAACTGCCTTCATC
  CATCACCGTGAGCAAGTGTGGAAGAGATGCATCAATGTTTGGCATGATGTGGGCCTTTTTGGCGTCCTGA
  ATGAAATTGCGAATTCAGAGGAAGAAGTGTTTGAGTGGGTAAAGACAGCCTGCAGCTGGGCCCTGGCTCT
  CTGTGGCCGCGCCTCCTCACTCCATGGCTCCCTGTTTCCACACCTCTCTCTCAGGAGTGAAGATCTGATT
  GCTGAATTTGCCCAAGTAACTAACTGGTCCAGCTGCTGCTTACGGGTCTTTGCCTGGCATCCTCACACCA
  ACAAGTTTGCAGTGGCCCTGCTGGACGACTCCATCCGTGTTTATAATGCCAACAGCACTATAGTTCCCTC
  CCTGAAGCACCGTCTGCAGCGAAATGTAGCAGCCTTGGCCTGGAAGCCCCTCAGTGCCTCTGTCTTGGCT
  GTGGCCTGCCAGAGCTGTATTCTCATCTGGACCCTAGACCCCACTTCCTTATCTACGAGACCCTCCTCTG
  GCTGCGCCCAGGTGTTGTCTCACCCTGGGCACACACCAGTCACTAGCTTGGCTTGGGCCCCCAATGGGGG
  GTGGCTGCTCTCAGCCTCACCTGTAGATGCTGTTATTCTGGTATGGGACGTTTCAACAGAGACTTGTGTC
  CCCCTTCCTTGGTTCCGAGGAGGAGGGGTTACCAATCTGCTGTGGTCCCCAGATGGCAGCAAAGTCCTGG
  CTACTACTCCTTCTGCCGTCTTTAGAGTCTGGGAGGCCCAGATGTGGACTTGTGAGGCGTGGCCAACTCT
  TTCTGGGCGCTGCCAGACTGGCTGCTGGAGTCCAGATGGAAACCGACTGCTGTTCACTGTATTGGGGGAA
  GCATTAATTTATTCCTTGTCATTCCCAGAACGATGTGGAACAGGGAAAGGACATGTTGGAGGTGCAAAGT
  CAGCAACCATCGTAGCAGATCTTTCTGAGACAACAATTCAGACCCCAGATGGAGAGGAGCGACTCGGGGG
  AGAGGCCCACTCCATGGTGTGGGACCCAAGTGGTGAACGTCTGGCTGTGCTCATGAAAGGAAACCCACAG
  GTTCAGGACGGGAATCCTGTCATCCTCCTTTTTCGAACTCGAAACAGCCCCGTGTTTGAACTGCTTCCCT
  GTGGTATTATTCAGGGGGAACCAGGAGCCCAAGCTCAGCTCATCACTTTCCATCCTTCCTTCAACAAAGG
  GGCCTTGCTTAGTGTGTGCTGGTCCACAGGCCGAATCACCCACATACCTCTGTACTTTGTCAATGCTCAG
  TTTCCACGCTTTAGCCCAGTACTTGGTCGAGCCCAGGAGCCTCCAGCTGGAGGGGGAGGCTCTATTCATG
  AAGTGCCACTATTTACTGAGACATCACCAACCTCTGCTCCTTGGGACCCTCTTCCAGGACAATCATCTGC
  TCAACCCCACTCCCCTCATTCCCACTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208691 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MCSLGLFPPPPPRGQVTLYEHNNELVTGNSYESPPPDFRGQWINLPVLHLTKDPLKAPGRLDHGTRTAFI
  HHREQVWKRCINVWHDVGLFGVLNEIANSEEEVFEWVKTACSWALALCGRASSLHGSLFPHLSLRSEDLI
  AEFAQVTNWSSCCLRVFAWHPHTNKFAVALLDDSIRVYNANSTIVPSLKHRLQRNVAALAWKPLSASVLA
  VACQSCILIWTLDPTSLSTRPSSGCAQVLSHPGHTPVTSLAWAPNGGWLLSASPVDAVILVWDVSTETCV
  PLPWFRGGGVTNLLWSPDGSKVLATTPSAVFRVWEAQMWTCEAWPTLSGRCQTGCWSPDGNRLLFTVLGE
  ALIYSLSFPERCGTGKGHVGGAKSATIVADLSETTIQTPDGEERLGGEAHSMVWDPSGERLAVLMKGNPQ
  VQDGNPVILLFRTRNSPVFELLPCGIIQGEPGAQAQLITFHPSFNKGALLSVCWSTGRITHIPLYFVNAQ
  FPRFSPVLGRAQEPPAGGGGSIHEVPLFTETSPTSAPWDPLPGQSSAQPHSPHSHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153416
• ORF Size: 1641 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153416.1, NM_153416.2, NP_700465.1
• RefSeq Size: 1816 bp
• RefSeq ORF: 1641 bp
• Locus ID: 223921
• UniProt ID: P58742
• Cytogenetics: 15 F3
• MW: 59.4 kDa
• Gene Summary: Plays a role in the normal development of the peripheral and central nervous system. Required for the correct localization of aurora kinase AURKA and the microtubule minus end-binding protein NUMA1 as well as a subset of AURKA targets which ensures proper spindle formation and timely chromosome alignment.[UniProtKB/Swiss-Prot Function]