Camkk2 (NM_145358) Mouse Tagged ORF Clone

CAT#: MR208650

Camkk2 (Myc-DDK-tagged) - Mouse calcium/calmodulin-dependent protein kinase kinase 2, beta (Camkk2), transcript variant 2

Product Images

Circular map for MR208650

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Camkk2
• Synonyms: 6330570N16Rik; AW061083; mKIAA0787
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208650 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCATCATGTGTCTCTAGCCAGCCCACCAGCGACCGGGTGGCCCCCCAGGATGAGCTGGGAAGTGGGG
  GTGGCAGCCGGGAAGGCCAGAAGCCCTGTGAGGCACTGCGGGGACTCTCATCCTTAAGTATCCACTTGGG
  CATGGAATCCTTCATCGTGGTCACCGAGTGTGAGCCAGGCCGGGGTGTGGACCTCAACCTGGCCAGAGAC
  CAGCCTCCGGAGGCCGATGGCCAGGAACTCCCCCTCGAAGCCTCGGACCCTGAGTCCCGGTCCCCGCTTT
  CTGGTCGCAAGATGTCCCTGCAGGAGCCGTCCCAGGGCGGGCCGGCATCCAGCAGCAACAGCCTGGACAT
  GAATGGACGCTGCATCTGCCCATCCCTGTCCTACTCACCAGCCAGCTCCCCACAGTCCTCTCCCCGGATG
  CCCCGGCGGCCCACAGTAGAGTCACACCACGTCTCCATTACCGGTTTGCAGGACTGCGTGCAGCTGAATC
  AGTACACCCTGAAGGATGAAATTGGAAAGGGCTCCTATGGTGTTGTCAAGCTGGCCTACAATGAAAATGA
  CAATACTTATTACGCAATGAAAGTGCTGTCCAAAAAGAAGCTGATCCGACAGGCTGGCTTCCCACGTCGC
  CCCCCACCACGAGGAGCTCGCCCAGCCCCAGGGGGCTGCATCCAGCCCAGGGGCCCCATCGAGCAAGTGT
  ACCAGGAGATTGCTATCCTCAAAAAGCTGGATCATCCCAACGTGGTGAAGCTGGTAGAGGTCCTGGATGA
  CCCTAATGAGGACCATCTGTACATGGTGTTTGAGTTGGTGAACCAAGGGCCTGTAATGGAAGTGCCCACC
  CTCAAGCCACTGTCCGAAGACCAGGCCCGCTTCTACTTCCAGGATCTGATCAAAGGCATAGAGTACTTGC
  ATTACCAGAAGATCATCCATCGGGACATCAAACCTTCCAACCTCCTGGTGGGGGAAGACGGGCACATCAA
  GATTGCCGACTTCGGTGTAAGCAACGAGTTCAAGGGCAGCGACGCCTTGCTGTCTAACACCGTGGGCACA
  CCTGCCTTCATGGCACCCGAGTCGCTCTCAGAGACCCGCAAGATCTTCTCCGGAAAGGCGTTGGATGTTT
  GGGCCATGGGTGTGACGCTGTACTGCTTTGTCTTTGGCCAGTGCCCTTTCATGGATGAACGAATCATGTG
  TTTGCACAGTAAGATCAAGAGTCAGGCCCTGGAGTTTCCCGACCAGCCCGATATAGCCGAAGACTTGAAA
  GATCTGATCACTCGGATGTTGGACAAAAATCCAGAGTCCAGGATTGTGGTGCCTGAAATCAAGCTGCACC
  CTTGGGTTACGAGGCACGGGGCCGAGCCATTGCCGTCGGAGGACGAGAACTGCACACTGGTCGAGGTGAC
  CGAAGAGGAGGTCGAGAATTCAGTCAAACACATTCCCAGCCTGGCAACTGTGATCCTGGTAAAGACCATG
  ATTCGAAAGCGCTCATTCGGGAACCCGTTCGAAGGTAGCCGCCGGGAGGAACGTTCCCTGTCAGCGCCTG
  GAAACCTGCTCACGAAGCAAGGCAGCGAAGACAGCCCCCGGGGCCCAGAGCCGGCCCCTGTGGGGGAGGA
  GGAAGTGCTCTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208650 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSCVSSQPTSDRVAPQDELGSGGGSREGQKPCEALRGLSSLSIHLGMESFIVVTECEPGRGVDLNLARD
  QPPEADGQELPLEASDPESRSPLSGRKMSLQEPSQGGPASSSNSLDMNGRCICPSLSYSPASSPQSSPRM
  PRRPTVESHHVSITGLQDCVQLNQYTLKDEIGKGSYGVVKLAYNENDNTYYAMKVLSKKKLIRQAGFPRR
  PPPRGARPAPGGCIQPRGPIEQVYQEIAILKKLDHPNVVKLVEVLDDPNEDHLYMVFELVNQGPVMEVPT
  LKPLSEDQARFYFQDLIKGIEYLHYQKIIHRDIKPSNLLVGEDGHIKIADFGVSNEFKGSDALLSNTVGT
  PAFMAPESLSETRKIFSGKALDVWAMGVTLYCFVFGQCPFMDERIMCLHSKIKSQALEFPDQPDIAEDLK
  DLITRMLDKNPESRIVVPEIKLHPWVTRHGAEPLPSEDENCTLVEVTEEEVENSVKHIPSLATVILVKTM
  IRKRSFGNPFEGSRREERSLSAPGNLLTKQGSEDSPRGPEPAPVGEEEVLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145358
• ORF Size: 1626 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145358.2, NP_663333.1
• RefSeq Size: 4860 bp
• RefSeq ORF: 1626 bp
• Locus ID: 207565
• UniProt ID: Q8C078
• Cytogenetics: 5 F
• MW: 59.6 kDa
• Gene Summary: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Phosphorylates CAMK1, CAMK4 and CAMK1D (By similarity). Efficiently phosphorylates 5'-AMP-activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca(2+) signals (By similarity). May play a role in neurite growth. Isoform 2 may promote neurite elongation, while isoform 1 may promoter neurite branching (By similarity). May be involved in hippocampal activation of CREB1.[UniProtKB/Swiss-Prot Function]