Npr3 (NM_001039181) Mouse Tagged ORF Clone

CAT#: MR208587

Npr3 (Myc-DDK-tagged) - Mouse natriuretic peptide receptor 3 (Npr3), transcript variant 2

Product Images

Circular map for MR208587

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Npr3
• Synonyms: ANP-C; ANPR-C; EF-2; lgj; NPR-C; stri
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208587 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGTCCTTGCTGCTGTTCACTTTCTCGGCGTGCGTGCTGCTGGCCCGGGTGCTGCTGGCTGGCGGCG
  CGAGCAGCGGCGCCGGGGACACCCGGCCAGGCAGCAGGCGCCGGGCGAGAGAGGCGCTGGCGGCTCAAAA
  GATCGAGGTGCTTGTTCTATTGCCCCGGGACGATTCGTACTTGTTCTCGCTGGCCCGGGTGAGGCCGGCC
  ATCGAGTACGCGCTGCGTAGCGTGGAGGGCAATGGCACCGGGAGGAAGCTGCTGCCGCCGGGCACTCGCT
  TCCAGGTGGCCTACGAAGACTCGGACTGCGGCAACCGCGCGCTCTTCAGTCTTGTGGACCGCGTGGCGGC
  GGCGCGCGGCGCCAAGCCGGATCTCATCCTGGGGCCCGTGTGCGAGTACGCGGCGGCGCCGGTGGCTCGG
  CTGGCGTCTCACTGGGACCTGCCGATGCTGTCCGCAGGAGCGCTGGCCGCCGGTTTCCAGCACAAGGACA
  CGGAATACTCGCACCTCACGCGCGTGGCGCCTGCCTACGCCAAGATGGGAGAGATGATGCTCGCTCTGTT
  TCGCCACCACCACTGGAGCCGTGCAGCCCTGGTCTACAGCGACGACAAACTCGAGAGGAACTGTTATTTC
  ACCCTCGAGGGGGTCCACGAGGTTTTTCAGGAGGAGGGGTTGCACACGTCTGCCTACAATTTCGACGAAA
  CCAAAGACTTGGACCTGGACGACATAGTGCGCTACATCCAAGGCAGCGAGCGAGTGGTGATCATGTGTGC
  CAGTGGTGACACCATTCGGAGAATCATGTTGGCGGTGCACAGACACGGCATGACCAGTGGAGACTACGCT
  TTCTTCAACATTGAACTCTTCAACAGTTCTTCCTACGGAGATGGCTCGTGGAGGAGAGGAGACAAACACG
  ACTCTGAAGCTAAACAAGCATACTCGTCCCTCCAAACAGTCACTCTACTGAGGACCGTGAAACCTGAGTT
  TGAGAAGTTTTCCATGGAGGTGAAAAGTTCTGTTGAGAAACAAGGGCTCAATGAGGAGGATTACGTGAAC
  ATGTTTGTTGAAGGGTTCCATGACGCCATCCTCCTCTACGTTCTGGCTTTACACGAAGTGCTCAGAGCTG
  GCTACAGCAAGAAGGATGGGGGGAAAATCATCCAGCAGACTTGGAACAGGACATTTGAAGGTATCGCTGG
  GCAGGTGTCCATAGATGCCAACGGGGACCGGTATGGGGACTTCTCTGTGGTTGCCATGACTGACACTGAA
  GCAGGCACCCAAGAGGTCATTGGTGATTACTTTGGGAAAGAAGGCCGGTTCCAAATGCGATCGAATGTCA
  AATATCCTTGGGGCCCTTTGAAACTGAGACTAGATGAGACCAGAATCGTGGAGCATACCAACAGCTCTCC
  TTGCAAATCATGTGGCCTAGAAGAATCTGCAGTGACAGGAATCGTTGTGGGGGCCCTACTAGGTGCTGGC
  TTGCTAATGGCCTTCTACTTTTTCAGGAAGAAATACAGAATAACCATTGAGAGGCGAAATCAGCAAGAGG
  AAAGCAACATCGGGAAGCATCGAGAGCTGCGGGAAGATTCCATCAGATCACATTTTTCGGTGGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208587 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRSLLLFTFSACVLLARVLLAGGASSGAGDTRPGSRRRAREALAAQKIEVLVLLPRDDSYLFSLARVRPA
  IEYALRSVEGNGTGRKLLPPGTRFQVAYEDSDCGNRALFSLVDRVAAARGAKPDLILGPVCEYAAAPVAR
  LASHWDLPMLSAGALAAGFQHKDTEYSHLTRVAPAYAKMGEMMLALFRHHHWSRAALVYSDDKLERNCYF
  TLEGVHEVFQEEGLHTSAYNFDETKDLDLDDIVRYIQGSERVVIMCASGDTIRRIMLAVHRHGMTSGDYA
  FFNIELFNSSSYGDGSWRRGDKHDSEAKQAYSSLQTVTLLRTVKPEFEKFSMEVKSSVEKQGLNEEDYVN
  MFVEGFHDAILLYVLALHEVLRAGYSKKDGGKIIQQTWNRTFEGIAGQVSIDANGDRYGDFSVVAMTDTE
  AGTQEVIGDYFGKEGRFQMRSNVKYPWGPLKLRLDETRIVEHTNSSPCKSCGLEESAVTGIVVGALLGAG
  LLMAFYFFRKKYRITIERRNQQEESNIGKHRELREDSIRSHFSVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039181
• ORF Size: 1605 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039181.1, NP_001034270.1
• RefSeq Size: 6924 bp
• RefSeq ORF: 1608 bp
• Locus ID: 18162
• Cytogenetics: 15 5.83 cM
• MW: 59.8 kDa
• Gene Summary: Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP (PubMed:10377427, PubMed:17951249). May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects (PubMed:10377427, PubMed:17951249). May regulate diuresis, blood pressure and skeletal development (PubMed:10377427). Does not have guanylate cyclase activity (PubMed:10377427).[UniProtKB/Swiss-Prot Function]