Traf6 (NM_009424) Mouse Tagged ORF Clone

CAT#: MR208489

Traf6 (Myc-DDK-tagged) - Mouse TNF receptor-associated factor 6 (Traf6)

Product Images

Circular map for MR208489

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Traf6
• Synonyms: 2310003F17Rik; AI851288; C630032O20Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208489 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCTCTTAAACTGTGAGAACAGCTGCGGGTCCAGCCAGTCGTCCAGTGACTGCTGCGCTGCCATGG
  CCGCCTCCTGCAGCGCTGCAGTGAAAGATGACAGCGTGAGTGGCTCTGCCAGCACCGGGAACCTCTCCAG
  CTCCTTCATGGAGGAGATCCAGGGCTACGATGTGGAGTTTGACCCACCTCTGGAGAGCAAGTATGAGTGT
  CCCATCTGCTTGATGGCTTTACGGGAAGCAGTGCAAACACCATGTGGCCACAGGTTCTGCAAAGCCTGCA
  TCATCAAATCCATAAGGGATGCAGGGCACAAGTGCCCAGTTGACAATGAAATACTGCTGGAAAATCAACT
  GTTTCCCGACAATTTTGCAAAGCGAGAGATTCTTTCCCTGACGGTAAAGTGCCCAAATAAAGGCTGTTTG
  CAAAAGATGGAACTGAGACATCTCGAGGATCATCAAGTACATTGTGAATTTGCTCTAGTGAATTGTCCCC
  AGTGCCAACGTCCTTTCCAGAAGTGCCAGGTTAATACACACATTATTGAGGATTGTCCCAGGAGGCAGGT
  TTCTTGTGTAAACTGTGCTGTGTCCATGGCATATGAAGAGAAAGAGATCCATGATCAAAGCTGTCCTCTG
  GCAAATATCATCTGTGAATACTGTGGTACAATCCTCATCAGAGAACAGATGCCTAATCATTATGATCTGG
  ACTGCCCAACAGCTCCAATCCCTTGCACATTCAGTGTTTTTGGCTGTCATGAAAAGATGCAGAGGAATCA
  CTTGGCACGACACTTGCAAGAGAATACCCAGTTGCACATGAGACTGTTGGCCCAGGCTGTTCATAATGTT
  AACCTTGCTTTGCGTCCGTGCGATGCCGCCTCTCCATCCCGGGGATGTCGTCCAGAGGACCCAAATTATG
  AGGAAACTATCAAACAGTTGGAGAGTCGCCTAGTAAGACAGGACCATCAGATCCGGGAGCTGACTGCCAA
  AATGGAAACTCAGAGTATGTACGTGGGCGAGCTCAAACGGACCATTCGGACCCTGGAGGACAAGGTTGCC
  GAAATGGAAGCACAGCAGTGTAACGGGATCTACATTTGGAAGATTGGCAACTTTGGGATGCACTTGAAAT
  CCCAAGAAGAGGAAAGACCTGTTGTCATCCATAGCCCTGGATTCTACACAGGCAGACCTGGGTACAAGCT
  GTGCATGCGCCTGCATCTTCAGTTACCGACAGCTCAGCGCTGTGCAAACTATATATCCCTTTTTGTCCAC
  ACAATGCAAGGAGAATATGACAGCCACCTCCCCTGGCCCTTCCAGGGTACAATACGCCTTACAATTCTCG
  ACCAGTCTGAAGCACTTATAAGGCAAAACCACGAAGAGGTCATGGACGCCAAACCAGAACTGCTTGCCTT
  TCAGCGACCCACAATCCCACGGAACCCCAAAGGTTTTGGCTATGTAACATTTATGCACCTGGAAGCCTTA
  AGACAGGGAACCTTCATTAAGGATGATACATTACTAGTGCGCTGTGAAGTCTCTACCCGCTTTGACATGG
  GTGGCCTTCGGAAGGAGGGTTTCCAGCCACGAAGTACTGATGCGGGGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208489 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLLNCENSCGSSQSSSDCCAAMAASCSAAVKDDSVSGSASTGNLSSSFMEEIQGYDVEFDPPLESKYEC
  PICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLTVKCPNKGCL
  QKMELRHLEDHQVHCEFALVNCPQCQRPFQKCQVNTHIIEDCPRRQVSCVNCAVSMAYEEKEIHDQSCPL
  ANIICEYCGTILIREQMPNHYDLDCPTAPIPCTFSVFGCHEKMQRNHLARHLQENTQLHMRLLAQAVHNV
  NLALRPCDAASPSRGCRPEDPNYEETIKQLESRLVRQDHQIRELTAKMETQSMYVGELKRTIRTLEDKVA
  EMEAQQCNGIYIWKIGNFGMHLKSQEEERPVVIHSPGFYTGRPGYKLCMRLHLQLPTAQRCANYISLFVH
  TMQGEYDSHLPWPFQGTIRLTILDQSEALIRQNHEEVMDAKPELLAFQRPTIPRNPKGFGYVTFMHLEAL
  RQGTFIKDDTLLVRCEVSTRFDMGGLRKEGFQPRSTDAGV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009424
• ORF Size: 1593 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009424.3, NP_033450.2
• RefSeq Size: 5536 bp
• RefSeq ORF: 1593 bp
• Locus ID: 22034
• UniProt ID: P70196
• Cytogenetics: 2 E2
• MW: 60.1 kDa
• Gene Summary: This gene encodes a member of the TNF receptor associated factor (TRAF) family of adaptor proteins that mediate signaling events from members of the TNF receptor and Toll/IL-1 receptor families to activate transcription factors such as NF-kappa-B and AP-1. The product of this gene is essential for perinatal and postnatal survival. Mice deficient in this protein exhibit osteopetrosis and defective in development of epidermal appendixes, normal B cell differentiation, lymph node organogenesis, interleukin-1 signaling, lipopolysaccharide signaling and neural tube closure. This protein possesses ubiquitin ligase activity. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2014]