Aldh2 (NM_009656) Mouse Tagged ORF Clone

CAT#: MR208315

Aldh2 (Myc-DDK-tagged) - Mouse aldehyde dehydrogenase 2, mitochondrial (Aldh2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aldh2
• Synonyms: Ahd-5; AHD-M1; Ahd5; ALDH-E2; ALDHI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208315 representing NM_009656
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGCGCCGCACTCACCACTGTCCGCCGCGGACCGCGCCTGAGCCGCCTGTTGTCCGCCGCCGCCA
  CCAGCGCGGTGCCAGCCCCCAACCATCAGCCTGAGGTCTTCTGCAACCAGATCTTCATTAACAATGAGTG
  GCACGACGCCGTCAGCAGGAAAACATTTCCCACCGTCAACCCTTCCACAGGGGAGGTCATCTGCCAGGTG
  GCCGAAGGGAACAAGGAGGACGTAGACAAGGCAGTGAAGGCTGCTCGTGCAGCCTTCCAGCTGGGCTCGC
  CCTGGCGCCGCATGGATGCATCTGACCGGGGCCGGCTGTTGTACCGATTGGCGGATCTCATTGAACGGGA
  CCGGACCTACCTAGCGGCCTTGGAGACCCTGGACAACGGCAAGCCTTATGTCATCTCGTACCTGGTGGAT
  TTGGACATGGTCCTGAAATGTCTCCGCTATTACGCTGGCTGGGCTGACAAGTACCATGGGAAAACCATTC
  CCATCGACGGCGACTTCTTCAGCTATACCCGCCATGAGCCTGTGGGCGTGTGTGGACAGATCATTCCGTG
  GAACTTCCCGCTCCTGATGCAAGCATGGAAACTGGGCCCAGCCCTGGCAACCGGGAACGTGGTGGTGATG
  AAGGTGGCCGAGCAGACACCGCTCACCGCGCTCTACGTGGCCAACTTGATCAAGGAGGCAGGCTTTCCCC
  CTGGCGTGGTCAATATCGTTCCCGGATTCGGCCCTACCGCCGGGGCTGCCATCGCATCCCATGAGGGTGT
  GGACAAAGTGGCGTTCACAGGCTCCACGGAGGTTGGTCACCTAATCCAGGTGGCCGCCGGGAGCAGCAAC
  CTCAAGAGAGTAACCCTGGAGCTGGGGGGAAAGAGTCCCAACATCATCATGTCCGACGCTGACATGGACT
  GGGCTGTGGAGCAGGCCCACTTTGCCCTGTTCTTCAACCAGGGCCAGTGCTGCTGCGCAGGCTCCCGGAC
  CTTCGTGCAGGAGAATGTGTATGACGAATTCGTGGAACGCAGCGTGGCTCGGGCCAAGTCTCGGGTGGTG
  GGGAACCCCTTCGACAGCCGGACGGAGCAGGGGCCTCAGGTGGATGAAACTCAGTTTAAGAAGATCCTCG
  GCTACATCAAATCGGGACAACAAGAAGGGGCGAAGCTGCTGTGTGGTGGGGGCGCTGCCGCGGACCGTGG
  CTACTTTATCCAGCCCACCGTGTTCGGGGACGTAAAAGACGGCATGACCATTGCCAAGGAGGAGATCTTT
  GGACCAGTGATGCAAATCCTCAAATTCAAGACCATCGAGGAGGTTGTGGGGCGGGCCAATGATTCTAAGT
  ATGGGCTGGCAGCCGCCGTCTTCACAAAGGACCTGGATAAAGCCAATTACCTGTCCCAAGCTCTGCAGGC
  TGGCACTGTGTGGATCAACTGCTACGATGTGTTTGGGGCCCAGTCTCCATTTGGGGGCTATAAGATGTCA
  GGGAGTGGCAGGGAGCTGGGCGAGTATGGCCTGCAGGCGTACACAGAAGTGAAGACGGTTACTGTCAAAG
  TGCCACAGAAGAACTCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208315 representing NM_009656
  Red=Cloning site Green=Tags(s)
  
  MLRAALTTVRRGPRLSRLLSAAATSAVPAPNHQPEVFCNQIFINNEWHDAVSRKTFPTVNPSTGEVICQV
  AEGNKEDVDKAVKAARAAFQLGSPWRRMDASDRGRLLYRLADLIERDRTYLAALETLDNGKPYVISYLVD
  LDMVLKCLRYYAGWADKYHGKTIPIDGDFFSYTRHEPVGVCGQIIPWNFPLLMQAWKLGPALATGNVVVM
  KVAEQTPLTALYVANLIKEAGFPPGVVNIVPGFGPTAGAAIASHEGVDKVAFTGSTEVGHLIQVAAGSSN
  LKRVTLELGGKSPNIIMSDADMDWAVEQAHFALFFNQGQCCCAGSRTFVQENVYDEFVERSVARAKSRVV
  GNPFDSRTEQGPQVDETQFKKILGYIKSGQQEGAKLLCGGGAAADRGYFIQPTVFGDVKDGMTIAKEEIF
  GPVMQILKFKTIEEVVGRANDSKYGLAAAVFTKDLDKANYLSQALQAGTVWINCYDVFGAQSPFGGYKMS
  GSGRELGEYGLQAYTEVKTVTVKVPQKNS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009656
• ORF Size: 1557 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009656.4, NP_033786.1
• RefSeq Size: 3883 bp
• RefSeq ORF: 1560 bp
• Locus ID: 11669
• UniProt ID: P47738
• Cytogenetics: 5 F
• MW: 56.5 kDa
• Gene Summary: Is capable of converting retinaldehyde to retinoic acid.[UniProtKB/Swiss-Prot Function]