Aldh1a2 (NM_009022) Mouse Tagged ORF Clone

CAT#: MR208298

Aldh1a2 (Myc-DDK-tagged) - Mouse aldehyde dehydrogenase family 1, subfamily A2 (Aldh1a2)

Product Images

Circular map for MR208298

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aldh1a2
• Synonyms: Aldh1a7; AV116159; Raldh1; Raldh2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208298 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTCCAGCGAGATCGCCATGCCGGGCGAGGTGAAGGCCGACCCCGCCGCGCTCATGGCCTCGCTGC
  AGCTCCTGCCGTCGCCCACGCCCAACCTCGAGATCAAGTACACCAAGATCTTTATTAACAATGAATGGCA
  GAACTCAGAGAGTGGGAGAGTGTTCCCTGTCTGTAATCCAGCCACAGGAGAGCAAGTGTGTGAAGTTCAA
  GAAGCAGACAAGGTGGATATAGACAAGGCAGTGCAAGCAGCCCGCCTTGCCTTCTCTCTTGGTTCTGTGT
  GGAGAAGGATGGATGCGTCTGAAAGAGGACGTCTGTTGGACAAGCTTGCAGACTTGGTGGAACGGGACAG
  GGCAACTCTTGCAACTATGGAATCCCTAAATGGCGGTAAGCCATTCCTGCAAGCTTTTTACATCGATTTG
  CAGGGAGTCATCAAAACCCTGAGGTATTATGCAGGCTGGGCTGATAAAATTCACGGAATGACCATTCCTG
  TAGATGGAGACTATTTTACTTTCACAAGACACGAGCCCATTGGAGTGTGTGGACAGATCATCCCGTGGAA
  CTTCCCCCTGCTGATGTTCACCTGGAAAATTGCTCCCGCATTGTGCTGTGGTAACACCGTGGTCATCAAA
  CCCGCTGAGCAGACACCGCTCAGTGCACTGTATATGGGAGCCCTCATCAAGGAGGCTGGCTTTCCACCCG
  GAGTCGTCAATATCCTGCCAGGGTATGGGCCAACAGCAGGGGCAGCAATCGCTTCTCACATCGGCATAGA
  CAAGATTGCATTCACAGGCTCTACTGAGGTTGGAAAGCTTATTCAAGAAGCAGCTGGAAGAAGTAACCTG
  AAGAGAGTGACCCTGGAACTCGGAGGGAAGAGCCCTAATATTATTTTTGCAGATGCTGACTTGGACTACG
  CTGTGGAGCAGGCTCACCAGGGTGTGTTCTTCAACCAAGGCCAGTGCTGCACCGCAGGGTCTCGCATCTT
  TGTGGAAGAATCCATCTATGAGGAATTTGTGAAAAGAAGTGTGGAAAGGGCCAAGAGGCGCATTGTGGGG
  AGCCCCTTTGATCCCACAACTGAGCAGGGTCCTCAGATCGATAAGAAACAATACAACAAAGTCCTGGAGC
  TTATCCAGAGCGGTGTGGCTGAGGGCGCCAAGCTGGAGTGCGGAGGCAAAGGGCTGGGAAGGAAGGGCTT
  CTTCATTGAGCCCACCGTGTTCTCCAACGTCACTGATGACATGCGGATTGCCAAGGAGGAGATCTTTGGT
  CCTGTTCAAGAAATTTTGAGGTTTAAGACTATGGATGAAGTTATAGAAAGAGCCAATAACTCAGACTTTG
  GACTTGTAGCAGCTGTCTTCACTAATGACATCAACAAGGCTCTCATGGTATCCTCCGCAATGCAAGCTGG
  GACAGTTTGGATCAATTGTTACAATGCCTTAAATGCGCAGAGCCCCTTTGGGGGGTTCAAGATGTCTGGA
  AATGGGAGAGAAATGGGTGAGTTTGGCTTACGGGAGTATTCAGAAGTAAAGACCGTGACGGTGAAGATCC
  CCCAGAAGAACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208298 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTSSEIAMPGEVKADPAALMASLQLLPSPTPNLEIKYTKIFINNEWQNSESGRVFPVCNPATGEQVCEVQ
  EADKVDIDKAVQAARLAFSLGSVWRRMDASERGRLLDKLADLVERDRATLATMESLNGGKPFLQAFYIDL
  QGVIKTLRYYAGWADKIHGMTIPVDGDYFTFTRHEPIGVCGQIIPWNFPLLMFTWKIAPALCCGNTVVIK
  PAEQTPLSALYMGALIKEAGFPPGVVNILPGYGPTAGAAIASHIGIDKIAFTGSTEVGKLIQEAAGRSNL
  KRVTLELGGKSPNIIFADADLDYAVEQAHQGVFFNQGQCCTAGSRIFVEESIYEEFVKRSVERAKRRIVG
  SPFDPTTEQGPQIDKKQYNKVLELIQSGVAEGAKLECGGKGLGRKGFFIEPTVFSNVTDDMRIAKEEIFG
  PVQEILRFKTMDEVIERANNSDFGLVAAVFTNDINKALMVSSAMQAGTVWINCYNALNAQSPFGGFKMSG
  NGREMGEFGLREYSEVKTVTVKIPQKNS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009022
• ORF Size: 1557 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009022.4, NP_033048.2
• RefSeq Size: 2264 bp
• RefSeq ORF: 1557 bp
• Locus ID: 19378
• UniProt ID: Q62148
• Cytogenetics: 9 39.85 cM
• MW: 56.6 kDa
• Gene Summary: Converts retinaldehyde to retinoic acid (PubMed:8797830). Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal (By similarity). Lacks activity with benzaldehyde, acetaldehyde and octanal (PubMed:8797830). Displays complete lack of activity with citral (By similarity).[UniProtKB/Swiss-Prot Function]