Aldh7a1 (NM_001127338) Mouse Tagged ORF Clone

CAT#: MR208207

Aldh7a1 (Myc-DDK-tagged) - Mouse aldehyde dehydrogenase family 7, member A1 (Aldh7a1), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aldh7a1
• Synonyms: Atq1; D18Wsu181e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208207 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTACTCTGCTGATCCATCATCCCCAGTATGCCTGGCTGCAAGACCTGGGGCTCCGCGAGGATAACG
  AGGGCGTGTATAATGGAAGCTGGGGCGGCCGGGGAGAGGTAATTACGACCTATTGTCCTGCTAACAATGA
  GCCAATAGCAAGAGTCCGACAGGCCAGCCTGAAGGACTATGAAGAAACCATCGGGAAAGCCAAGAAAGCC
  TGGAACATCTGGGCAGATATTCCTGCCCCAAAGCGAGGAGAAATAGTCAGAAAGATTGGCGATGCCTTCC
  GGGAGAAGATTCAACTACTGGGAAGACTGGTGTCTTTGGAGATGGGGAAAATCCTCGTGGAAGGAATAGG
  CGAGGTTCAGGAGTACGTGGACGTCTGTGACTATGCTGCTGGCTTGTCGAGGATGATCGGGGGACCCACC
  TTGCCTTCTGAAAGACCCGGCCATGCTCTCATCGAAATGTGGAATCCCTTAGGCTTGGTGGGAATCATCA
  CTGCCTTCAATTTCCCCGTGGCTGTGTTTGGCTGGAACAATGCCATAGCCCTGATCACAGGGAATGTCTG
  CCTTTGGAAAGGAGCACCGACTACGTCCCTCGTTAGTGTGGCTGTCACAAAGATCATAGCCCAGGTTTTG
  GAGGACAACCTGCTGCCCGGTGCCATTTGTTCCCTGGTTTGTGGTGGAGCAGATATCGGCACAACGATGG
  CCAGAGATGAGCGTGTGAACCTGCTGTCCTTCACTGGGAGCACTCAGGTGGGGAAGGAGGTGGCCCTCAT
  GGTGCAGGAGAGGTTTGGGAAAAGCTTGTTGGAGCTTGGAGGAAACAACGCCATTATTGCTTTCGAGGAC
  GCGGACCTCAGCTTGGTTGTTCCATCAGTTCTGTTTGCCGCCGTGGGAACAGCTGGGCAAAGGTGTACCA
  CTGTGAGGCGACTGTTTTTGCACGAAAGCATCCATAATGAAGTTGTGGACAGACTGAGAAGTGCCTACTC
  ACAGATCCGTGTCGGGAACCCCTGGGACCCCAATATCCTCTATGGACCGCTCCATACCAAACAGGCAGTG
  AGCATGTTTGTGAGAGCCGTGGAAGAAGCCAAGAAACAAGGGGGCACAGTGGTCTATGGGGGCAAGGTCA
  TGGACCACCCTGGCAATTACGTGGAACCCACCATTGTGACCGGTCTTGCCCATGATGCGCCCATTGTTCA
  CCAGGAGACTTTTGCCCCAATCCTCTATGTCTTCAAATTCCAGGATGAAGAAGAGGTCTTTGAATGGAAC
  AATGAAGTAAAACAGGGACTTTCAAGTAGTATCTTTACCAAAGATTTGGGCAGAATCTTCCGCTGGCTTG
  GACCTAAAGGTTCCGACTGTGGCATCGTGAACGTCAATATTCCTACCAGCGGGGCTGAGATTGGTGGTGC
  GTTTGGCGGAGAGAAGCACACTGGCGGTGGCCGGGAGTCTGGCAGCGACGCCTGGAAGCAGTACATGAGG
  AGATCCACATGTACCATCAACTACAGCACGTCCCTCCCTCTGGCTCAGGGAATCAAGTTTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208207 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSTLLIHHPQYAWLQDLGLREDNEGVYNGSWGGRGEVITTYCPANNEPIARVRQASLKDYEETIGKAKKA
  WNIWADIPAPKRGEIVRKIGDAFREKIQLLGRLVSLEMGKILVEGIGEVQEYVDVCDYAAGLSRMIGGPT
  LPSERPGHALIEMWNPLGLVGIITAFNFPVAVFGWNNAIALITGNVCLWKGAPTTSLVSVAVTKIIAQVL
  EDNLLPGAICSLVCGGADIGTTMARDERVNLLSFTGSTQVGKEVALMVQERFGKSLLELGGNNAIIAFED
  ADLSLVVPSVLFAAVGTAGQRCTTVRRLFLHESIHNEVVDRLRSAYSQIRVGNPWDPNILYGPLHTKQAV
  SMFVRAVEEAKKQGGTVVYGGKVMDHPGNYVEPTIVTGLAHDAPIVHQETFAPILYVFKFQDEEEVFEWN
  NEVKQGLSSSIFTKDLGRIFRWLGPKGSDCGIVNVNIPTSGAEIGGAFGGEKHTGGGRESGSDAWKQYMR
  RSTCTINYSTSLPLAQGIKFQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001127338
• ORF Size: 1536 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127338.1, NP_001120810.1
• RefSeq Size: 3105 bp
• RefSeq ORF: 1536 bp
• Locus ID: 110695
• UniProt ID: Q9DBF1
• Cytogenetics: 18 30.55 cM
• MW: 55.6 kDa
• Gene Summary: Multifunctional enzyme mediating important protective effects. Metabolizes betaine aldehyde to betaine, an important cellular osmolyte and methyl donor. Protects cells from oxidative stress by metabolizing a number of lipid peroxidation-derived aldehydes. Involved in lysine catabolism (By similarity).[UniProtKB/Swiss-Prot Function]