Cyp2j6 (NM_010008) Mouse Tagged ORF Clone

CAT#: MR208057

Cyp2j6 (Myc-DDK-tagged) - Mouse cytochrome P450, family 2, subfamily j, polypeptide 6 (Cyp2j6)

Product Images

Circular map for MR208057

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyp2j6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR208057 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCGCTGCTACCGGCTCCTTGTTAGCCACGATCTGGGCAGCGCTCCATCCCAGGACTCTGTTGGTGG
  CTGCAGTCACCTTCCTGCTTCTGGCTGATTACTTCAAAAACCGGCGCCCCAAGAACTATCCCCCAGGGCC
  TTGGGGTCTGCCTTTCGTGGGCAACATATTCCAGTTGGACTTTGGGCAGCCCCACCTCTCAATCCAGCCG
  TTGGTGAAGAAATATGGGAATATTTTTAGCCTGAATCTTGGTGACATAACTTCAGTGGTCATAACTGGAC
  TGCCCTTAATCAAAGAAGCACTTACTCAAATGGAACAAAACATTATGAATCGTCCTCTAAGTGTTATGCA
  AGAACGTATATCTAATAAAAATGGATTGATCTTCTCCAGTGGCCAAATATGGAAGGAGCAAAGAAGGTTT
  GCCCTGATGACACTGAGGAACTTTGGACTGGGAAAGAAGAGCTTAGAGGAGCGCATGCAGGAGGAGGCCT
  CCCACCTAGTGGAAGCCATAAGAGAGGAGGAAGGAAAACCTTTCAACCCTCACTTCAGTATCAACAATGC
  AGTTTCCAATATCATTTGCTCTGTCACCTTTGGGGAGCGCTTTGACTACCATGACAGTAGATTTCAGGAG
  ATGCTGAGGTTACTGGACGAGGTTATGTATTTGGAGACAACAATGATTAGTCAGCTGTATAATATTTTTC
  CATGGATAATGAAATACATCCCTGGATCACATCAAAAAGTTTTCAGAAACTGGGAAAAACTTAAATTGTT
  TGTCTCTTGTATGATTGATGATCACCGGAAAGACTGGAACCCCGATGAACCAAGAGACTTCATTGATGCT
  TTCCTCAAGGAAATGACAAAGTACCCAGAGAAGACTACAAGTTTCAATGAAGAAAACCTTATCTGCAGCA
  CTTTGGACCTCTTCTTTGCTGGAACAGAGACAACATCAACTACATTACGCTGGGCTCTGCTCTACATGGC
  CCTCTACCCAGAAGTCCAAGAAAAAGTACAGGCAGAGATTGATAGGGTGATTGGCCAGAAGAGGGCAGCA
  AGACTTGCTGATAGAGAATCCATGCCCTATACCAATGCTGTCATCCACGAGGTGCAGAGGATGGGCAATA
  TCATCCCCTTGAATGTTCCCAGGGAAGTAGCAATGGATACCAATTTGAATGGATTTCATCTGCCAAAGGG
  TACAATGGTTCTAACAAACCTAACTGCACTGCACAGAGACCCAAAAGAGTGGGCCACTCCAGATGTGTTC
  AATCCAGAGCACTTTTTGGAGAATGGACAGTTTAAGAAGAGAGAATCCTTTCTGCCCTTCTCAATGGGAA
  AGCGAGCTTGCCTGGGAGAACAACTGGCCAGGTCTGAGCTGTTCATTTTCTTCACTAGCCTTATGCAAAA
  ATTCACCTTCAATCCCCCAATCAATGAGAAGCTGAGCCCAAAGTTCAGAAATGGCCTCACACTTTCTCCA
  GTCAGCCACCGCATCTGTGCTGTCCCTAGACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR208057 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLAATGSLLATIWAALHPRTLLVAAVTFLLLADYFKNRRPKNYPPGPWGLPFVGNIFQLDFGQPHLSIQP
  LVKKYGNIFSLNLGDITSVVITGLPLIKEALTQMEQNIMNRPLSVMQERISNKNGLIFSSGQIWKEQRRF
  ALMTLRNFGLGKKSLEERMQEEASHLVEAIREEEGKPFNPHFSINNAVSNIICSVTFGERFDYHDSRFQE
  MLRLLDEVMYLETTMISQLYNIFPWIMKYIPGSHQKVFRNWEKLKLFVSCMIDDHRKDWNPDEPRDFIDA
  FLKEMTKYPEKTTSFNEENLICSTLDLFFAGTETTSTTLRWALLYMALYPEVQEKVQAEIDRVIGQKRAA
  RLADRESMPYTNAVIHEVQRMGNIIPLNVPREVAMDTNLNGFHLPKGTMVLTNLTALHRDPKEWATPDVF
  NPEHFLENGQFKKRESFLPFSMGKRACLGEQLARSELFIFFTSLMQKFTFNPPINEKLSPKFRNGLTLSP
  VSHRICAVPRQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010008
• ORF Size: 1506 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010008.1, NM_010008.2, NM_010008.3, NM_010008.4, NP_034138.2
• RefSeq Size: 3595 bp
• RefSeq ORF: 1506 bp
• Locus ID: 13110
• UniProt ID: O54750
• Cytogenetics: 4 44.62 cM
• MW: 57.8 kDa