Aldh9a1 (NM_019993) Mouse Tagged ORF Clone

CAT#: MR207926

Aldh9a1 (Myc-DDK-tagged) - Mouse aldehyde dehydrogenase 9, subfamily A1 (Aldh9a1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aldh9a1
• Synonyms: AA139417; Abaldh; ESTM40; TMABA-DH; Tmabadh
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207926 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTACTGGCACCTTCGTCGTGTCGCAGCCGCTCAACTACCGCGGCGGGGCCCGTGTGGAGCCGGTGG
  ATGCTTCCGGCACGGAGAAAGCGTTCGAGCCAGCCACCGGCCGAGTGATTGCCACTTTCGCGTGTTCAGG
  AGAAAAGGAAGTAAACTTGGCCGTTGAGAATGCAAAGGCTGCCTTTAAACTCTGGAGTAAGAAAAGTGGC
  CTGGAGCGCTGCCAAGTCCTCCTAGAGGCTGCCCGGATCATCAAGGAGCGGAAGGATGAAATTGCCACCG
  TGGAGACCATCAACAACGGGAAGTCCATATTTGAGGCCCGCCTGGATGTTGACACTTGCTGGCAGTGCCT
  GGAATACTATGCAGGGCTGGCTGCATCCATGGCAGGCGAGCACATTCAGCTCCCAGGAGGGTCCTTCGGC
  TACACCCGAAGAGAGCCGCTTGGGGTGTGTGTGGGGATAGGAGCATGGAACTATCCCTTCCAGATCGCCT
  GTTGGAAGTCTGCTCCGGCTCTGGCTTGTGGTAATGCCATGATCTTTAAGCCTTCTCCCTTCACGCCTGT
  GTCTGCTCTGCTCCTGGCTGAGATCTACACCAAAGCGGGTGCGCCTCCGGGGCTCTTCAACGTGGTGCAA
  GGTGGGGCTGCCACAGGCCAGTTTCTGTGTCATCATCGCGAGGTGGCCAAAATCTCCTTCACCGGAAGTG
  TGCCCACTGGCGTGAAGATCATGGAGATGTCCGCTAAGGGAGTCAAGCCCATTACTTTGGAACTTGGGGG
  CAAATCTCCTCTCATCATCTTCTCAGACTGTAATATGGAGAATGCTGTGAAGGGGGCCCTGATGGCCAAC
  TTCCTCACACAAGGACAGGTCTGCTGCAATGGGACTAGAGTCTTTGTGCAAAAGGAAATTGCTGATAAAT
  TCATAAATGAGGTTGTGAAGCAGACTCAGAAGATAAAACTTGGAGACCCCCTTCTGGAAGATACGAGGAT
  GGGCCCACTCATCAACGCCCCACATCTGGAACGAGTCCTCGGGTTTGTCAAGTTGGCAAAGGAGCAGGGT
  GCTACTGTGCTGTGTGGTGGAGAGGTGTATGTACCAGAGGACCCCAAATTAAAACATGGATATTACATGA
  CACCTTGCATTTTAACTAATTGCAGAGACGACATGACTTGTGTAAAAGAAGAGATCTTTGGACCAGTCAT
  GTCCATTTTAACATTTGGAACTGAAGCCGAGGTTCTAGAGCGAGCTAATGACACCACTTTTGGACTAGCA
  GCTGGGGTCTTCACCAGGGACATCCAGAGGGCACACCGGGTGGCGGCTGAGCTGCAGGCTGGAACGTGCT
  ACATTAACAACTATAATGTCAGCCCAGTGGAGCTGCCCTTTGGTGGCTACAAGAAGTCAGGATTTGGCAG
  AGAGAATGGCCGTGTGACGATTGAGTACTACTCTCAGCTGAAGACGGTGTGTGTGGAGATGGGCGACGTG
  GAGTCAGCCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207926 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSTGTFVVSQPLNYRGGARVEPVDASGTEKAFEPATGRVIATFACSGEKEVNLAVENAKAAFKLWSKKSG
  LERCQVLLEAARIIKERKDEIATVETINNGKSIFEARLDVDTCWQCLEYYAGLAASMAGEHIQLPGGSFG
  YTRREPLGVCVGIGAWNYPFQIACWKSAPALACGNAMIFKPSPFTPVSALLLAEIYTKAGAPPGLFNVVQ
  GGAATGQFLCHHREVAKISFTGSVPTGVKIMEMSAKGVKPITLELGGKSPLIIFSDCNMENAVKGALMAN
  FLTQGQVCCNGTRVFVQKEIADKFINEVVKQTQKIKLGDPLLEDTRMGPLINAPHLERVLGFVKLAKEQG
  ATVLCGGEVYVPEDPKLKHGYYMTPCILTNCRDDMTCVKEEIFGPVMSILTFGTEAEVLERANDTTFGLA
  AGVFTRDIQRAHRVAAELQAGTCYINNYNVSPVELPFGGYKKSGFGRENGRVTIEYYSQLKTVCVEMGDV
  ESAF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019993
• ORF Size: 1485 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019993.2
• RefSeq Size: 2834 bp
• RefSeq ORF: 1557 bp
• Locus ID: 56752
• UniProt ID: Q9JLJ2
• Cytogenetics: 1 H2.3
• MW: 53.5 kDa
• Gene Summary: Converts gamma-trimethylaminobutyraldehyde into gamma-butyrobetaine with high efficiency (in vitro). Can catalyze the irreversible oxidation of a broad range of aldehydes to the corresponding acids in an NAD-dependent reaction, but with low efficiency.[UniProtKB/Swiss-Prot Function]