Arih2 (NM_011790) Mouse Tagged ORF Clone

CAT#: MR207887

Arih2 (Myc-DDK-tagged) - Mouse ariadne homolog 2 (Drosophila) (Arih2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Arih2
• Synonyms: AI843547; ARI2; TRIAD1; UIP48
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207887 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTGGACATGAACAGCCAAGGGTCAGATAGCAATGAAGAGGACTATGACCCAAACTGTGAGGAAG
  AGGAAGAAGAGGAAGAGGACCCTGGGGACATAGAGGACTACTATGTGGGAGTAGCCAGTGATGTGGAGCA
  GCAGGGGGCTGATGCTTTTGATCCTGAAGAGTATCAGTTCACTTGCTTGACCTATAAGGAGTCTGAGGGC
  GCCCTCCATGAACATATGACCAGCTTAGCCTCTGTCTTAAAGGTATCTCATTCAGTTGCAAAACTTATAT
  TAGTTAATTTCCACTGGCAAGTCTCAGAGATTCTGGACAGGTACAGGTCTAATTCTGCTCAGCTGCTTGT
  TGAGGCTCGAGTTCAACCTAATCCATCCAAACATGTCCCCACAGCCCATCCCCCTCACCACTGTGCAGTG
  TGTATGCAATTTGTGCGGAAGGAAAACCTACTCTCTCTGGCCTGCCAACATCAGTTTTGCCGAAGCTGTT
  GGGAACAGCATTGCTCAGTGCTTGTCAAAGATGGTGTTGGTGTAGGAATCTCATGTATGGCTCAGGATTG
  TCCACTTCGAACACCAGAGGACTTTGTATTTCCCTTGCTTCCCAATGAAGAATTGAGAGATAAATACAGG
  CGCTACCTCTTTAGGGACTATGTGGAGAGCCATTTCCAGCTCCAGCTGTGCCCCGGTGCAGACTGCCCCA
  TGGTTATCCGGGTACAGGAGCCTAGAGCCCGCCGAGTGCAGTGCAATCGGTGCAGCGAGGTCTTCTGTTT
  CAAGTGTCGACAGATGTATCACGCCCCCACAGACTGCGCCACAATCCGGAAATGGCTCACCAAGTGTGCA
  GACGACTCCGAAACGGCTAACTACATTAGTGCTCACACTAAAGACTGTCCCAAGTGCAACATCTGCATCG
  AGAAGAATGGAGGCTGTAATCACATGCAATGCTCCAAGTGTAAGCACGACTTCTGCTGGATGTGTCTAGG
  AGACTGGAAGACGCATGGTAGTGAGTACTATGAATGTAGTCGGTACAAGGAGAATCCTGACATTGTCAAC
  CAGAGCCAGCAAGCACAGGCCAGGGAGGCCCTCAAGAAGTACTTGTTCTACTTTGAGAGGTGGGAAAATC
  ACAACAAAAGCTTGCAGCTGGAGGCACAAACATATGAGCGGATTCACGAGAAGATTCAGGAGAGGGTTAT
  GAACAACCTGGGAACTTGGATTGACTGGCAGTACCTACAGAATGCTGCCAAGCTCTTAGCCAAGTGTCGA
  TATACCCTGCAGTACACCTATCCATATGCATACTACATGGAATCCGGTCCCAGGAAGAAGCTGTTTGAAT
  ACCAGCAGGCTCAGCTGGAGGCTGAGATCGAAAACCTTTCATGGAAAGTTGAGCGTGCAGACAGCTACGA
  CAGAGGGGACTTAGAAAACCAGATGCACATAGCAGAGCAGCGGAGGAGAACCCTGCTAAAGGATTTTCAC
  GACACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207887 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSVDMNSQGSDSNEEDYDPNCEEEEEEEEDPGDIEDYYVGVASDVEQQGADAFDPEEYQFTCLTYKESEG
  ALHEHMTSLASVLKVSHSVAKLILVNFHWQVSEILDRYRSNSAQLLVEARVQPNPSKHVPTAHPPHHCAV
  CMQFVRKENLLSLACQHQFCRSCWEQHCSVLVKDGVGVGISCMAQDCPLRTPEDFVFPLLPNEELRDKYR
  RYLFRDYVESHFQLQLCPGADCPMVIRVQEPRARRVQCNRCSEVFCFKCRQMYHAPTDCATIRKWLTKCA
  DDSETANYISAHTKDCPKCNICIEKNGGCNHMQCSKCKHDFCWMCLGDWKTHGSEYYECSRYKENPDIVN
  QSQQAQAREALKKYLFYFERWENHNKSLQLEAQTYERIHEKIQERVMNNLGTWIDWQYLQNAAKLLAKCR
  YTLQYTYPYAYYMESGPRKKLFEYQQAQLEAEIENLSWKVERADSYDRGDLENQMHIAEQRRRTLLKDFH
  DT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011790
• ORF Size: 1479 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011790.5
• RefSeq Size: 3924 bp
• RefSeq ORF: 1479 bp
• Locus ID: 23807
• UniProt ID: Q9Z1K6
• Cytogenetics: 9 F2
• MW: 57.7 kDa
• Gene Summary: E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 (By similarity). Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates: associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets (By similarity). The initial ubiquitin is then elongated (By similarity). E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the ECS complex. Mediates 'Lys-6', 'Lys-48'-and 'Lys-63'-linked polyubiquitination. May play a role in myelopoiesis (By similarity).[UniProtKB/Swiss-Prot Function]