Tmprss2 (NM_015775) Mouse Tagged ORF Clone

CAT#: MR207852

Reviews ()
Write a review
  • TrueORF®

Tmprss2 (Myc-DDK-tagged) - Mouse transmembrane protease, serine 2 (Tmprss2)



  "NM_015775" in other vectors (4)

Interest in protein/lysate? Submit request here!

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 398.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Tmprss2
Synonyms D16Ertd61e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR207852 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATTGAACTCAGGGTCACCTCCAGGAATCGGACCTTGCTATGAGAACCACGGGTATCAGTCTGAGC
ACATCTGTCCTCCGAGACCACCAGTGGCTCCCAATGGCTACAACTTGTATCCAGCCCAGTACTACCCATC
TCCAGTGCCTCAGTATGCTCCGAGGATTACAACGCAAGCCTCAACATCTGTCATCCACACACATCCCAAG
TCCTCAGGAGCACTGTGCACCTCAAAGTCTAAGAAATCGCTGTGTTTAGCCCTCGCCCTGGGCACTGTCC
TCACGGGAGCTGCTGTGGCTGCTGTCTTGCTTTGGAGGTTCTGGGACAGCAACTGTTCTACGTCTGAGAT
GGAGTGTGGGTCTTCAGGCACATGCATCAGCTCTTCTCTCTGGTGTGACGGGGTAGCACATTGTCCCAAC
GGAGAAGATGAGAACCGTTGTGTTCGTCTCTACGGACAAAGCTTCATCCTCCAGGTTTACTCATCTCAGA
GGAAAGCCTGGTATCCCGTGTGCCAGGATGATTGGAGTGAGAGCTACGGGAGAGCAGCATGTAAAGACAT
GGGATACAAGAACAATTTTTATTCTAGCCAAGGGATACCAGACCAGAGCGGGGCAACGAGCTTTATGAAG
CTGAATGTGAGCTCAGGCAACGTTGACCTCTATAAAAAACTCTACCACAGTGACTCATGTTCATCCCGCA
TGGTGGTTTCTTTGCGCTGTATAGAATGCGGGGTTCGCTCAGTGAAACGCCAGAGCAGGATTGTGGGTGG
ATTGAATGCCTCACCAGGAGACTGGCCCTGGCAGGTCAGCCTGCACGTCCAAGGCGTCCACGTCTGCGGA
GGCTCCATCATCACCCCCGAGTGGATTGTGACGGCCGCCCACTGTGTGGAAGAACCCCTCAGCAGCCCGA
GGTACTGGACGGCATTTGCGGGAATTCTGAGACAGTCTCTCATGTTCTATGGAAGTAGACACCAGGTAGA
AAAAGTAATTTCCCATCCAAATTACGACTCTAAGACCAAGAATAACGACATTGCTCTCATGAAGCTGCAG
ACACCTTTGGCTTTTAATGATCTAGTGAAGCCAGTGTGTCTGCCGAACCCAGGCATGATGCTAGACCTAG
ACCAGGAATGCTGGATTTCGGGGTGGGGGGCCACCTATGAGAAAGGGAAGACCTCGGACGTGTTGAATGC
TGCCATGGTACCCTTGATCGAGCCCTCCAAATGTAATAGTAAATACATATACAACAACCTAATCACACCA
GCCATGATCTGTGCCGGCTTCCTCCAGGGGTCTGTCGACTCTTGCCAGGGAGACAGTGGAGGGCCGCTGG
TTACTTTGAAGAATGGGATCTGGTGGCTGATTGGGGACACGAGCTGGGGCTCGGGCTGTGCCAAGGCACT
CAGACCTGGAGTATACGGGAACGTGACGGTATTTACAGATTGGATCTACCAGCAAATGAGGGCGAACAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR207852 protein sequence
Red=Cloning site Green=Tags(s)

MALNSGSPPGIGPCYENHGYQSEHICPPRPPVAPNGYNLYPAQYYPSPVPQYAPRITTQASTSVIHTHPK
SSGALCTSKSKKSLCLALALGTVLTGAAVAAVLLWRFWDSNCSTSEMECGSSGTCISSSLWCDGVAHCPN
GEDENRCVRLYGQSFILQVYSSQRKAWYPVCQDDWSESYGRAACKDMGYKNNFYSSQGIPDQSGATSFMK
LNVSSGNVDLYKKLYHSDSCSSRMVVSLRCIECGVRSVKRQSRIVGGLNASPGDWPWQVSLHVQGVHVCG
GSIITPEWIVTAAHCVEEPLSSPRYWTAFAGILRQSLMFYGSRHQVEKVISHPNYDSKTKNNDIALMKLQ
TPLAFNDLVKPVCLPNPGMMLDLDQECWISGWGATYEKGKTSDVLNAAMVPLIEPSKCNSKYIYNNLITP
AMICAGFLQGSVDSCQGDSGGPLVTLKNGIWWLIGDTSWGSGCAKALRPGVYGNVTVFTDWIYQQMRANS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_015775
ORF Size 1473 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_015775.2, NP_056590.2
RefSeq Size 3175 bp
RefSeq ORF 1473 bp
Locus ID 50528
UniProt ID Q9JIQ8
Cytogenetics 16 57.53 cM
MW 53.5 kDa
Gene Summary Serine protease that proteolytically cleaves and activates the viral spike glycoproteins which facilitate virus-cell membrane fusions. The spike proteins are synthesized and maintained in precursor intermediate folding states and proteolysis permits the refolding and energy release required to create stable virus-cell linkages and membrane coalescence. Facilitates human SARS coronavirus (SARS-CoV) infection via two independent mechanisms, proteolytic cleavage of ACE2, which might promote viral uptake, and cleavage of coronavirus spike glycoprotein which activates the glycoprotein for cathepsin L-independent host cell entry. Proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV). Essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9) and is involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity.[UniProtKB/Swiss-Prot Function]

Other Versions

Other products for "Tmprss2"
Frequently bought together (4)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 30 ul

USD 165.00


pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 627.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 149.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 55.00

Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.