Aldh3b1 (NM_026316) Mouse Tagged ORF Clone

CAT#: MR207483

Aldh3b1 (Myc-DDK-tagged) - Mouse aldehyde dehydrogenase 3 family, member B1 (Aldh3b1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aldh3b1
• Synonyms: 1700001N19Rik; ALDH4; ALDH7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR207483 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCGTTTGAAGACAAGCTTCAGCAGCTGCGGGAGGCCTTCAAGGAGGGGCGGACGAGGTCAGCTG
  AGTTCCGAGCGGCACAGCTGCAAGGCCTGAGCCACTTCCTCCGAGACAACAAACAACAGCTGCAGGAGGC
  GTTGGCCCAAGACCTGCACAAGTCAGCCTTCGAGGCCGAGGTGTCTGAGATTGCCATCAGTCAGGCCGAG
  GTGGACCTGGCCCTCAGGAACCTGCGGTCTTGGATGAAGGATGAGAAAGTGTCCAAGAATCTGGCTACAC
  AGCTGGACTCAGCCTTCATCCGGAAGGAGCCCTTCGGTCTGGTGCTTATCATCGTGCCCTGGAATTATCC
  CATTAACCTGACACTCGTGCCGCTGGTGGGGGCCATAGCTGCAGGGAATTGTGTGGTGCTCAAGCCCTCA
  GAGATCAGCAAGGCCACTGAAAAGATACTGGCTGAGGTCCTGCCCCGCTATCTGGACCAGAGCTGCTTTA
  CTGTGGTACTGGGTGGGCGTCAGGAGACCGGGCAGCTGCTGGAACACAAGTTCGACTACATCTTCTTCAC
  AGGGAATGCTTATGTAGGCAAGATCGTCATGGCTGCTGCTGCCAAACACCTGACACCCATCACCCTGGAG
  CTGGGGGGTAAGAACCCCTGCTATGTGGATGACAACTGCGACCCCCAGATAGTGGCCAACCGTGTGGCCT
  GGTTCCGCTACTTTAATGCCGGCCAGACCTGTGTGGCCCCCGATTATATCCTGTGTAGCCAGGAGATGCA
  GGAGCGGTTGGTGCCTGCCCTACAGAATGCCATCACACGTTTCTATGGAGACAACCCACAGACCTCCCCC
  AACCTGGGCAGAATCATCAACCAGAAGCATTTCAAGCGACTCCAGGGATTGCTGGGCTGTGGCCGTGTGG
  CCATCGGTGGCCAGAGTGATGAGGGAGAGCGCTACATTGCCCCCACAGTGTTAGTGGACGTGCAGGAGAC
  AGAGCCTGTGATGCAGGAGGAGATCTTTGGGCCCATCCTGCCCCTGGTGACTGTGAGAAGCCTGGATGAA
  GCCATCGAGTTCATGAACCGGCGGGAGAAGCCACTGGCGCTGTATGCCTTCTCCAAGAGAAGCCAGGTTA
  TCAAACAGGTGTTGGCCCGGACCAGCAGCGGGGGATTCTGTGGGAATGATGGCTTCATGCACATGACCCT
  GTCCAGCCTTCCTTTTGGAGGAGTGGGAACCAGCGGGATGGGCAGGTACCACGGCAAGTTCTCCTTTGAC
  ACCTTCTCCAACCAGCGTGCCTGTCTGCTGCGCAGCCCGGGAATGGAGAAAATCAATGACTTGCGCTACC
  CACCCTACTCCAGTCGCAACCTCAGGGTGTTGTTGGTGGCCATGGAGGAGAGGTGCTGCAGCTGCACCCT
  GCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR207483 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDSFEDKLQQLREAFKEGRTRSAEFRAAQLQGLSHFLRDNKQQLQEALAQDLHKSAFEAEVSEIAISQAE
  VDLALRNLRSWMKDEKVSKNLATQLDSAFIRKEPFGLVLIIVPWNYPINLTLVPLVGAIAAGNCVVLKPS
  EISKATEKILAEVLPRYLDQSCFTVVLGGRQETGQLLEHKFDYIFFTGNAYVGKIVMAAAAKHLTPITLE
  LGGKNPCYVDDNCDPQIVANRVAWFRYFNAGQTCVAPDYILCSQEMQERLVPALQNAITRFYGDNPQTSP
  NLGRIINQKHFKRLQGLLGCGRVAIGGQSDEGERYIAPTVLVDVQETEPVMQEEIFGPILPLVTVRSLDE
  AIEFMNRREKPLALYAFSKRSQVIKQVLARTSSGGFCGNDGFMHMTLSSLPFGGVGTSGMGRYHGKFSFD
  TFSNQRACLLRSPGMEKINDLRYPPYSSRNLRVLLVAMEERCCSCTLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026316
• ORF Size: 1407 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026316.3
• RefSeq Size: 1915 bp
• RefSeq ORF: 1407 bp
• Locus ID: 67689
• UniProt ID: Q80VQ0
• Cytogenetics: 19 A
• MW: 52.3 kDa
• Gene Summary: Oxidizes medium and long chain saturated and unsaturated aldehydes (PubMed:25286108). Metabolizes also benzaldehyde (By similarity). Low activity towards acetaldehyde and 3,4-dihydroxyphenylacetaldehyde (By similarity). May not metabolize short chain aldehydes. Can use both NADP(+) and NAD(+) as electron acceptor (By similarity). May have a protective role against the cytotoxicity induced by lipid peroxidation (By similarity).[UniProtKB/Swiss-Prot Function]