Ndufv1 (NM_133666) Mouse Tagged ORF Clone

CAT#: MR207415

  • TrueORF®

Ndufv1 (Myc-DDK-tagged) - Mouse NADH dehydrogenase (ubiquinone) flavoprotein 1 (Ndufv1), nuclear gene encoding mitochondrial protein

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK


  "NM_133666" in other vectors (4)


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Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

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Frequently bought together (5)
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Other products for "Ndufv1"

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Ndufv1
Synonyms CI-51kD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR207415 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTAGCGGCACGGCATTTTCTCGGCGGGTTGGTTCCCGTGCGGGTATCTGTGCGTTTCAGCAGCGGCA
CGACAGCACCCAAGAAAACCTCATTTGGCTCACTGAAGGATGAAGACCGGATCTTTACCAACCTGTATGG
GCGCCATGACTGGAGGCTGAAAGGTGCCCTGAGACGGGGTGACTGGTACAAGACAAAGGAGATCCTGCTG
AAGGGGCCTGACTGGATCTTGGGTGAGATGAAGACATCAGGTTTACGGGGCCGTGGTGGTGCTGGCTTCC
CCACTGGCCTCAAGTGGAGCTTCATGAATAAGCCCTCAGATGGCAGGCCCAAGTATCTGGTGGTGAATGC
TGACGAGGGAGAGCCAGGCACCTGTAAGGACCGAGAGATCATGCGTCATGACCCTCACAAGCTGGTGGAA
GGCTGCCTTGTGGGAGGCCGGGCCATGGGAGCCCGGGCCGCCTATATCTACATCCGAGGGGAATTCTACA
ACGAGGCCTCCAATTTGCAGGTAGCTATCCGAGAGGCCTATGAAGCAGGTCTGATTGGCAAGAATGCTTG
TGGCTCCGACTATGATTTTGATGTGTTTGTGGTGCGTGGGGCTGGGGCCTACATCTGTGGAGAAGAGACG
GCACTTATTGAATCCATTGAAGGCAAGCAGGGAAAGCCACGCCTGAAGCCGCCCTTTCCAGCAGATGTGG
GAGTGTTTGGATGCCCCACAACTGTGGCCAATGTGGAGACAGTGGCTGTGTCCCCCACCATTTGCCGTCG
TGGGGGCACCTGGTTTGCTGGCTTTGGCCGAGAACGCAATTCAGGTACCAAATTGTTTAACATCTCTGGC
CATGTCAACCACCCCTGCACTGTGGAGGAAGAGATGTCTGTGCCACTGAAAGAGCTGATTGAGAAACATG
CTGGTGGTGTCACAGGTGGCTGGGACAACCTCCTTGCTGTGATTCCTGGTGGCTCATCTACTCCACTGAT
TCCCAAATCTGTGTGTGAGACCGTGCTAATGGACTTCGATGCACTGGTGCAGGCTCAGACAGGCCTGGGC
ACGGCTGCAGTTATTGTTATGGATCGCTCGACAGACATTGTGAAAGCCATCGCTCGTCTCATTGAGTTCT
ACAAGCATGAGAGCTGTGGCCAGTGTACCCCGTGCCGTGAGGGCGTTGACTGGATGAACAAGGTGATGGC
CCGATTTGTGAAGGGAGATGCCCGGCCAGCTGAGATTGACTCCCTGTGGGAGATCAGCAAGCAGATAGAA
GGCCACACCATTTGTGCTCTGGGTGATGGGGCTGCCTGGCCAGTACAGGGTCTGATCCGACATTTCAGGC
CAGAGCTTGAGGATCGGATGCAACGGTTTGCCCAGCAGCACCGGGCCTGGCAGGCAGCCTCG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR207415 protein sequence
Red=Cloning site Green=Tags(s)

MLAARHFLGGLVPVRVSVRFSSGTTAPKKTSFGSLKDEDRIFTNLYGRHDWRLKGALRRGDWYKTKEILL
KGPDWILGEMKTSGLRGRGGAGFPTGLKWSFMNKPSDGRPKYLVVNADEGEPGTCKDREIMRHDPHKLVE
GCLVGGRAMGARAAYIYIRGEFYNEASNLQVAIREAYEAGLIGKNACGSDYDFDVFVVRGAGAYICGEET
ALIESIEGKQGKPRLKPPFPADVGVFGCPTTVANVETVAVSPTICRRGGTWFAGFGRERNSGTKLFNISG
HVNHPCTVEEEMSVPLKELIEKHAGGVTGGWDNLLAVIPGGSSTPLIPKSVCETVLMDFDALVQAQTGLG
TAAVIVMDRSTDIVKAIARLIEFYKHESCGQCTPCREGVDWMNKVMARFVKGDARPAEIDSLWEISKQIE
GHTICALGDGAAWPVQGLIRHFRPELEDRMQRFAQQHRAWQAAS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_133666
ORF Size 1395 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_133666.1
RefSeq Size 1608 bp
RefSeq ORF 1395 bp
Locus ID 17995
UniProt ID Q91YT0
Cytogenetics 19 A
MW 50.8 kDa
Gene Summary This gene encodes a subunit of the NADH-ubiquinone oxidoreductase (complex I) enzyme, which is a large, multimeric protein. It is the first enzyme complex in the mitochondrial electron transport chain and catalyzes the transfer of electrons from NADH to the electron acceptor ubiquinone. The proton gradient created by electron transfer drives the conversion of ADP to ATP. This gene is a core subunit and is conserved in prokaryotes and eukaryotes. The human ortholog of this protein has been characterized. It has consensus motifs for NADH, flavin mononucleotide, and iron-sulfur binding sites and participates in the oxidation of NADH as part of the dehydrogenase module of complex I. In humans, deficiencies in complex I are associated with myopathies, encephalomyopathies, and neurodegenerative disorders. [provided by RefSeq, Jun 2013]

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