Tpbg (NM_011627) Mouse Tagged ORF Clone

CAT#: MR206796

Tpbg (Myc-DDK-tagged) - Mouse trophoblast glycoprotein (Tpbg), transcript variant 1

Product Images

Circular map for MR206796

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tpbg
• Synonyms: 5T4; AW495680; WAIF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206796 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGGGGCGGGCTCCCGGGGCCCCTCCGCCGGGGACGGACGGCTGAGGTTGGCAAGGCTGGCGCTAG
  TGCTGCTGGGTTGGGTCTCCGCGTCGGCCCCCAGCTCTTCGGTACCCTCGTCTTCCACCTCCCCGGCAGC
  CTTCCTGGCCTCGGGGTCTGCGCAGCCTCCGCCAGCCGAGAGATGCCCCGCGGCGTGCGAGTGCTCCGAG
  GCGGCGCGCACGGTTAAGTGCGTGAACCGCAACCTGCTGGAGGTGCCGGCGGATCTACCGCCTTACGTGC
  GCAACCTTTTCCTTACCGGCAACCAGATGACCGTGCTCCCCGCGGGCGCCTTCGCCCGCCAGCCGCCGCT
  CGCCGACCTGGAGGCGCTCAACCTCAGCGGCAACCACCTGAAGGAGGTGTGTGCAGGTGCCTTCGAGCAT
  CTGCCGGGTCTGCGCCGGCTTGACCTCAGCCACAACCCTCTCACCAACCTCAGCGCCTTCGCCTTTGCGG
  GCAGCAACGCCAGCGTCTCGGCCCCCAGCCCCCTGGAGGAGCTGATCCTGAATCACATCGTGCCCCCTGA
  GGATCAGAGGCAGAACGGGAGCTTCGAGGGTATGGTGGCCTTCGAAGGCATGGTGGCAGCAGCTCTGCGC
  TCAGGCCTTGCACTCCGAGGTCTTACATGCCTGGAGCTAGCCAGCAATCACTTTCTTTTCCTGCCTCGGG
  ACTTACTAGCCCAACTGCCGAGTCTCAGATACCTGGACCTCAGGAACAATTCCCTGGTGAGCCTGACCTA
  CGCATCCTTCCGCAACCTGACACACCTCGAAAGCCTCCACTTGGAGGACAATGCCCTCAAGGTCCTTCAC
  AACTCCACCTTGGCTGAGTGGCAAGGCCTGGCTCATGTCAAGGTGTTCCTGGACAACAATCCCTGGGTTT
  GCGACTGCTACATGGCTGACATGGTGGCTTGGCTTAAAGAGACAGAGGTGGTGCCAGATAAAGCCAGGCT
  TACCTGCGCATTCCCGGAGAAGATGAGGAATCGTGGCCTCTTAGACCTCAACAGCTCTGACCTGGACTGT
  GACGCTGTCCTTCCCCAATCCCTGCAGACTTCCTATGTCTTCCTAGGTATTGTTTTAGCTCTGATAGGCG
  CTATTTTCCTCCTCGTTTTGTATTTGAACCGTAAAGGCATAAAAAAGTGGATGCATAACATCAGAGATGC
  CTGCAGGGATCACATGGAAGGGTATCATTACAGATACGAAATCAATGCGGACCCCAGATTAACAAATCTT
  AGTTCCAACTCGGATGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206796 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPGAGSRGPSAGDGRLRLARLALVLLGWVSASAPSSSVPSSSTSPAAFLASGSAQPPPAERCPAACECSE
  AARTVKCVNRNLLEVPADLPPYVRNLFLTGNQMTVLPAGAFARQPPLADLEALNLSGNHLKEVCAGAFEH
  LPGLRRLDLSHNPLTNLSAFAFAGSNASVSAPSPLEELILNHIVPPEDQRQNGSFEGMVAFEGMVAAALR
  SGLALRGLTCLELASNHFLFLPRDLLAQLPSLRYLDLRNNSLVSLTYASFRNLTHLESLHLEDNALKVLH
  NSTLAEWQGLAHVKVFLDNNPWVCDCYMADMVAWLKETEVVPDKARLTCAFPEKMRNRGLLDLNSSDLDC
  DAVLPQSLQTSYVFLGIVLALIGAIFLLVLYLNRKGIKKWMHNIRDACRDHMEGYHYRYEINADPRLTNL
  SSNSDV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011627
• ORF Size: 1281 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011627.1, NM_011627.2, NM_011627.3, NM_011627.4, NP_035757.2
• RefSeq Size: 3618 bp
• RefSeq ORF: 1281 bp
• Locus ID: 21983
• UniProt ID: Q9Z0L0
• Cytogenetics: 9 E3.1
• MW: 46.4 kDa
• Gene Summary: May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.[UniProtKB/Swiss-Prot Function]