Spock2 (NM_052994) Mouse Tagged ORF Clone

CAT#: MR206742

Spock2 (Myc-DDK-tagged) - Mouse sparc/osteonectin, cwcv and kazal-like domains proteoglycan 2 (Spock2)

Product Images

Circular map for MR206742

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Spock2
• Synonyms: AA407235; Gcap26; mKIAA0275
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206742 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGCTCCAGGCTCCGGGCGGCTGGCTCTGCCGCTGCTGCTCCTCGCCGTGGTCGCCTTGGCTGAAG
  GCGACGCCAAGGGGCTCAAGGAGGGCGAGACCCCCGGCAATTTCATGGAGGACGAGCAATGGCTGTCGTC
  CATCTCGCAGTACAGCGGCAAGATTAAGCACTGGAACCGCTTCCGAGACGAGGTAGAAGATGATTACATC
  AAGAGCTGGGAAGACAATCAGCAAGGAGATGAAGCTCTGGATACCACCAAGGACCCCTGCCAGAAGGTGA
  AGTGCAGTCGCCACAAGGTGTGTGTTGCTCAAGGCTACCAGCGCGCCATGTGCATCAGCCGCAAGAAGTT
  GGAGCACAGGATCAAGCAGCCTTCTCTGAAACTCCACGGAGGCAAAGACTCCGTCTGCAAGCCCTGTCAT
  ATGGCCCAGCTCGCTTCAGTCTGTGGCTCCGACGGCCATACCTACAGCTCTGTGTGTAAGCTGGAGCAGC
  AGGCATGCCTAAGCAGCAAGCAGCTGGCGGTGAGATGTGAGGGCCCCTGTCCCTGTCCCACGGAGCAGTC
  TACTGCGTCTACCACCGACAGCAAGTCAGAGACCTGCACAGGGCAGGACCTGGCCGACCTGGGGGATAGG
  CTCCGTGACTGGTTCCAGCTCCTTCGTGAGAACTCCAAGCAGAACGGCTCAGCCAACAGTGCAACTAACC
  CCGCAGGGTTGGACAAGAGCCTGGGGGCCAGTTGCAAGGACTCCATCGGTTGGATGTTCTCTAAGCTGGA
  CACTAGTGGAGACCTCTTCCTGGACCAGACAGAACTGGCTGCCATCAACCTGGACAAATACGAGGTCTGC
  ATCCGCCCCTTCTTCAACTCCTGTGATACCTACAAAGATGGCCGCGTCTCTACTGCTGAGTGGTGCTTCT
  GTTTCTGGAGGGAGAAGCCCCCCTGCCTGGCAGAGCTGGAGCGCACACAGATCCAGGAGGCAGCCAAGAA
  GAAGCCAGGTGTCTTCATCCCGAGCTGTGATGAAGATGGCTACTACCGGAAGATGCAGTGTGACCAGAGC
  AGAGGTGACTGCTGGTGTGTGGACCAGCTGGGCCTGGAGCTGACCGGCACACGCATGCATGGGACCCCTG
  ACTGTGATGACATCGTGGGTTTCTCCGGGGACTTTGGAAGCGGCGTTGGCTGGGAGGATGAGGAAGAAAA
  GGAGACAGAAGAGGCAGGGGAGGAGGCCGAGGAGGAGGAGGGTGAGGCGGGTGAGGCAGATGATGGAGGC
  TACATCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206742 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRAPGSGRLALPLLLLAVVALAEGDAKGLKEGETPGNFMEDEQWLSSISQYSGKIKHWNRFRDEVEDDYI
  KSWEDNQQGDEALDTTKDPCQKVKCSRHKVCVAQGYQRAMCISRKKLEHRIKQPSLKLHGGKDSVCKPCH
  MAQLASVCGSDGHTYSSVCKLEQQACLSSKQLAVRCEGPCPCPTEQSTASTTDSKSETCTGQDLADLGDR
  LRDWFQLLRENSKQNGSANSATNPAGLDKSLGASCKDSIGWMFSKLDTSGDLFLDQTELAAINLDKYEVC
  IRPFFNSCDTYKDGRVSTAEWCFCFWREKPPCLAELERTQIQEAAKKKPGVFIPSCDEDGYYRKMQCDQS
  RGDCWCVDQLGLELTGTRMHGTPDCDDIVGFSGDFGSGVGWEDEEEKETEEAGEEAEEEEGEAGEADDGG
  YIW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052994
• ORF Size: 1272 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052994.2, NP_443720.1
• RefSeq Size: 3931 bp
• RefSeq ORF: 1272 bp
• Locus ID: 94214
• UniProt ID: Q9ER58
• Cytogenetics: 10 B4
• MW: 46.9 kDa
• Gene Summary: May participate in diverse steps of neurogenesis. Binds calcium (By similarity).[UniProtKB/Swiss-Prot Function]