Acp2 (NM_007387) Mouse Tagged ORF Clone

CAT#: MR206733

Acp2 (Myc-DDK-tagged) - Mouse acid phosphatase 2, lysosomal (Acp2)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acp2
• Synonyms: Acp; Acp-2; LAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206733 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCAGACAGACTGGTTGGAGCCAGGCGGCTCTTCTCCAGTTCCTTCTTGGCATGTGCCTAACGG
  TGATGCCACCCATACAGGCCCGGAGTCTACGCTTTGTTACCTTGCTGTATCGACATGGAGATCGGTCACC
  AGTGAAGACATATCCCAAGGACCCCTATCAGGAAGAGAAATGGCCCCAGGGATTTGGTCAGCTAACCAAG
  GAAGGGATGCTACAGCACTGGGAGCTGGGCCAGGCCCTGCGGCAACGCTACCATGGCTTTCTGAACACCT
  CTTACCACAGGCAAGAGGTTTATGTGCGAAGCACAGACTTCGATCGTACTCTCATGAGTGCTGAGGCCAA
  CCTGGCTGGACTCTTCCCTCCCAATGAAGTTCAGCACTTCAACCCTAACATTTCATGGCAGCCTATCCCT
  GTTCACACTGTGCCCATCACTGAAGACAGGTTGCTGAAGTTTCCTTTGGGTCCATGTCCCCGTTATGAGC
  AGCTGCAGAATGAGACTCGGCAGACACCAGAGTATCAGAACAGAAGTATTCAGAATGCACAATTTCTGAA
  CATGGTGGCCAACGAGACAGGGCTTACAAACGTGACCCTAGAGACCATCTGGAATGTGTATGACACACTC
  TTTTGTGAGCAAACCCATGGGCTGCTTCTGCCGCCCTGGGCCTCACCCCAAACCGTGCAGCGTCTGAGCC
  AGCTAAAGGACTTCAGCTTCCTCTTCCTCTTCGGGATCCACGAGCAAGTACAGAAGGCCCGGCTTCAGGG
  GGGAGTTCTGCTGGCTCAGATATTGAAGAACCTGACCCTAATGGCAACTACCTCTCAATTCCCTAAGCTT
  CTGGTTTATTCCGCGCATGACACTACCCTGGTTGCTCTGCAAATGGCATTGAATGTCTACAATGGGAAGC
  AAGCTCCCTATGCTTCCTGCCACATATTTGAACTGTACCAGGAAGATAATGGGAATTTCTCAGTCGAGAT
  GTACTTTCGGAATGACAGTAAGAAGGCACCCTGGCCTCTGATCCTGCCTGGCTGTCCTCACCGTTGCCCA
  CTGCAGGATTTCCTTCGCCTCACAGAGCCTGTCATACCCAAGGACTGGCAGAAGGAGTGCCAGCTAGCAA
  ATGATACTGCAGACACAGAGGTAATTGTGGCATTGGCTGTCTGTGGCTCCATCCTCTTCCTTCTCATAGT
  GTTGCTCCTCACCATCCTCTTCCGGATGCAGGCCCAGCCTCCTGGCTACCACCATGTTGCAGACAGGGAA
  GACCATGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206733 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGRQTGWSQAALLQFLLGMCLTVMPPIQARSLRFVTLLYRHGDRSPVKTYPKDPYQEEKWPQGFGQLTK
  EGMLQHWELGQALRQRYHGFLNTSYHRQEVYVRSTDFDRTLMSAEANLAGLFPPNEVQHFNPNISWQPIP
  VHTVPITEDRLLKFPLGPCPRYEQLQNETRQTPEYQNRSIQNAQFLNMVANETGLTNVTLETIWNVYDTL
  FCEQTHGLLLPPWASPQTVQRLSQLKDFSFLFLFGIHEQVQKARLQGGVLLAQILKNLTLMATTSQFPKL
  LVYSAHDTTLVALQMALNVYNGKQAPYASCHIFELYQEDNGNFSVEMYFRNDSKKAPWPLILPGCPHRCP
  LQDFLRLTEPVIPKDWQKECQLANDTADTEVIVALAVCGSILFLLIVLLLTILFRMQAQPPGYHHVADRE
  DHA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007387
• ORF Size: 1272 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007387.2
• RefSeq Size: 4669 bp
• RefSeq ORF: 1272 bp
• Locus ID: 11432
• UniProt ID: P24638
• Cytogenetics: 2 50.54 cM
• MW: 48.5 kDa
• Gene Summary: The protein encoded by this gene belongs to the histidine acid phosphatase family, which hydrolyze orthophosphoric monoesters to alcohol and phosphate. This protein is localized to the lysosomal membrane, and is chemically and genetically distinct from the red cell acid phosphatase. Mice lacking this gene showed multiple defects, including bone structure alterations, lysosomal storage defects, and an increased tendency towards seizures. An enzymatically-inactive allele of this gene showed severe growth retardation, hair-follicle abnormalities, and an ataxia-like phenotype. Two isoforms are predicted to be produced from the same mRNA by the use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. [provided by RefSeq, Oct 2017]