Ass1 (NM_007494) Mouse Tagged ORF Clone

CAT#: MR206472

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  • TrueORF®

Ass1 (Myc-DDK-tagged) - Mouse argininosuccinate synthetase 1 (Ass1)



  "NM_007494" in other vectors (3)

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Reconstitution Protocol

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USD 488.00


Availability*
In Stock

Size
    • 10 ug


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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Ass1
Synonyms AA408052; ASS; Ass-1; fold
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR206472 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCAGCAAGGGCTCTGTGGTTCTGGCCTACAGTGGTGGCCTGGACACCTCCTGCATCCTCGTGTGGC
TGAAGGAACAAGGCTATGATGTCATCGCCTACCTGGCCAACATTGGCCAGAAGGAAGACTTTGAGGAAGC
CAGGAAGAAGGCGCTGAAGCTTGGGGCCAAAAAGGTGTTCATTGAGGATGTGAGCAAGGAATTTGTGGAA
GAGTTCATCTGGCCTGCTGTCCAGTCCAGTGCACTCTACGAGGACCGCTATCTCCTGGGCACCTCTCTCG
CCAGGCCTTGCATAGCTCGCAGACAGGTGGAGATTGCCCAGCGTGAAGGGGCCAAGTATGTGTCTCACGG
CGCCACGGGAAAGGGGAATGACCAGGTCCGCTTTGAGCTCACCTGCTACTCACTGGCACCCCAGATTAAG
GTCATCGCTCCCTGGAGGATGCCTGAGTTTTACAACCGGTTCAAGGGCCGAAATGATCTGATGGAGTATG
CAAAGCAACACGGAATCCCCATCCCTGTCACCCCCAAGAGCCCCTGGAGTATGGATGAAAACCTCATGCA
CATCAGCTATGAGGCTGGGATCCTGGAAAACCCCAAGAATCAAGCACCTCCGGGTCTCTACACAAAAACT
CAGGACCCTGCCAAAGCACCCAACAGCCCAGATGTCCTTGAGATAGAATTCAAAAAAGGGGTCCCTGTGA
AGGTGACCAACATCAAAGATGGCACAACCCGCACCACATCCCTGGAACTCTTCATGTACCTGAACGAAGT
TGCGGGCAAGCACGGAGTGGGTCGCATTGACATCGTGGAGAACCGCTTCATTGGAATGAAGTCCCGAGGT
ATCTACGAGACCCCAGCAGGGACCATCCTTTACCACGCTCATTTAGACATAGAGGCCTTCACGATGGATC
GGGAAGTACGCAAAATCAAGCAGGGCCTGGGCCTCAAATTCGCAGAGCTCGTATACACAGGTTTCTGGCA
CAGCCCTGAATGTGAATTTGTTCGCCACTGTATCCAGAAGTCCCAGGAGCGGGTAGAAGGGAAGGTGCAG
GTGTCTGTCTTCAAGGGCCAAGTGTACATCCTCGGTCGGGAGTCTCCACTTTCACTCTACAATGAAGAGC
TGGTGAGCATGAACGTACAGGGCGACTATGAGCCCATCGACGCCACTGGCTTCATCAATATCAACTCGCT
CAGGCTGAAGGAGTACCATCGCCTTCAGAGCAAGGTCACTGCCAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR206472 protein sequence
Red=Cloning site Green=Tags(s)

MSSKGSVVLAYSGGLDTSCILVWLKEQGYDVIAYLANIGQKEDFEEARKKALKLGAKKVFIEDVSKEFVE
EFIWPAVQSSALYEDRYLLGTSLARPCIARRQVEIAQREGAKYVSHGATGKGNDQVRFELTCYSLAPQIK
VIAPWRMPEFYNRFKGRNDLMEYAKQHGIPIPVTPKSPWSMDENLMHISYEAGILENPKNQAPPGLYTKT
QDPAKAPNSPDVLEIEFKKGVPVKVTNIKDGTTRTTSLELFMYLNEVAGKHGVGRIDIVENRFIGMKSRG
IYETPAGTILYHAHLDIEAFTMDREVRKIKQGLGLKFAELVYTGFWHSPECEFVRHCIQKSQERVEGKVQ
VSVFKGQVYILGRESPLSLYNEELVSMNVQGDYEPIDATGFININSLRLKEYHRLQSKVTAK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_007494
ORF Size 1239 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_007494.2
RefSeq Size 1631 bp
RefSeq ORF 1239 bp
Locus ID 11898
UniProt ID P16460
Cytogenetics 2 21.81 cM
MW 46.6 kDa
Gene Summary One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.[UniProtKB/Swiss-Prot Function]

Other Versions

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Frequently bought together (5)
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