Adrm1 (NM_019822) Mouse Tagged ORF Clone

CAT#: MR206404

Adrm1 (Myc-DDK-tagged) - Mouse adhesion regulating molecule 1 (Adrm1)

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adrm1
• Synonyms: 1110063P18Rik; 2510006J17Rik; AA408205; ARM-1; Arm1; AU043535; Gp110; Rpn13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206404 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGACTTCAGGCGCTCTGTTCCCAAGCCTGGTGCCCGGCTCTCGGGGGTCTTCTACCAAATATTTGG
  TGGAGTTCCGGGCAGGAAAAATGTCATTAAAAGGAACTACGGTCACCCCAGATAAACGGAAAGGTCTCGT
  GTACATCCAGCAGACGGACGACTCCCTTATTCACTTCTGTTGGAAAGACAGGACCTCTGGGACCGTGGAG
  GATGACTTGATTATCTTTCCTGATGACTGTGAGTTCAAGCGGGTACCTCAGTGCCCCAGTGGGAGGGTCT
  ACGTGCTCAAGTTTAAGGCAGGGTCCAAGCGGCTCTTCTTCTGGATGCAGGAGCCCAAGACTGACCAAGA
  TGAGGAGCACTGCCGGAAAGTCAACGAGTGTCTGAACAACCCCCCCATGCCTGGGTCACTGGGAGCAAGT
  GGGAGTAGTGGCCATGAGCTTTCAGCACTGGGCGGTGAGGGTGGCCTGCAGAGCCTGTTGGGGAACATGA
  GTCACAGCCAGCTTATGCAGCTCATCGGACCAGCCGGCCTGGGAGGACTGGGTGGACTTGGGGCCCTCAC
  TGGGCCAGGCCTGGCCAGCTTGCTGGGGAGCAGTGGACCTCCAGCCAGCAGCTCTTCATCCAGCTCCCGG
  AGCCAGTCGGCAGCCGTCACCCCATCCTCCTCCACCTCTTCCGCTCGCGCCACCCCAGCCCCTTCTGCCC
  CAGCAGCTGCCTCGGCAACCAGCCCAAGCCCCGCACCCAGCTCAGGTAATGGAACCAGCACAGCAGCCAG
  CCCGACCCAGCCCATCCAGCTGAGCGACCTCCAGAGCATTCTGGCCACTATGAACGTGCCGGCAGGGCCA
  GGAGGCAGCCAGCAAGTGGATCTGGCGAGTGTGCTGACCCCAGAGATCATGGCTCCCATCCTTGCCAATG
  CAGACGTTCAGGAGCGCCTGCTGCCCTACCTGCCCTCTGGGGAGTCTCTGCCCCAGACTGCAGATGAGAT
  CCAGAACACATTAACCTCGCCCCAGTTCCAGCAGGCCCTGGGTATGTTCAGTGCGGCCTTGGCCTCAGGA
  CAGCTTGGCCCTCTCATGTGCCAGTTCGGCCTTCCTGCAGAGGCTGTTGAGGCCGCCAACAAAGGTGATG
  TGGAAGCATTTGCCAAAGCCATGCAGAACAATGCCAAATCGGACCCAAAGGAGGGCGACACAAAAGACAA
  GAAAGACGAAGAAGAAGATATGAGTCTAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206404 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTTSGALFPSLVPGSRGSSTKYLVEFRAGKMSLKGTTVTPDKRKGLVYIQQTDDSLIHFCWKDRTSGTVE
  DDLIIFPDDCEFKRVPQCPSGRVYVLKFKAGSKRLFFWMQEPKTDQDEEHCRKVNECLNNPPMPGSLGAS
  GSSGHELSALGGEGGLQSLLGNMSHSQLMQLIGPAGLGGLGGLGALTGPGLASLLGSSGPPASSSSSSSR
  SQSAAVTPSSSTSSARATPAPSAPAAASATSPSPAPSSGNGTSTAASPTQPIQLSDLQSILATMNVPAGP
  GGSQQVDLASVLTPEIMAPILANADVQERLLPYLPSGESLPQTADEIQNTLTSPQFQQALGMFSAALASG
  QLGPLMCQFGLPAEAVEAANKGDVEAFAKAMQNNAKSDPKEGDTKDKKDEEEDMSLD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019822
• ORF Size: 1224 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019822.3, NP_062796.2
• RefSeq Size: 1429 bp
• RefSeq ORF: 1224 bp
• Locus ID: 56436
• UniProt ID: Q9JKV1
• Cytogenetics: 2 H4
• MW: 42.1 kDa
• Gene Summary: Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. Within the complex, functions as a proteasomal ubiquitin receptor. Engages and thus activates 19S-associated deubiquitinases UCHL5 and PSMD14 during protein degradation. UCHL5 reversibly associate with the 19S regulatory particle whereas PSMD14 is an intrinsic subunit of the proteasome lid subcomplex.[UniProtKB/Swiss-Prot Function]