Ido1 (NM_008324) Mouse Tagged ORF Clone

CAT#: MR206394

Ido1 (Myc-DDK-tagged) - Mouse indoleamine 2,3-dioxygenase 1 (Ido1)

Product Images

Circular map for MR206394

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ido1
• Synonyms: Ido; Indo
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206394 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACTCAGTAAAATATCTCCTACAGAAGGTTCTAGAAGGATCCTTGAAGACCACCACATAGATGAAG
  ATGTGGGCTTTGCTCTACCACATCCACTGGTGGAGCTGCCCGACGCATACAGCCCCTGGGTCCTTGTGGC
  TAGAAATCTGCCTGTGCTGATTGAGAACGGGCAGCTTCGAGAAGAAGTTGAAAAGCTGCCCACACTGAGC
  ACGGACGGACTGAGAGGACACAGGTTACAGCGCCTGGCACACCTGGCCCTGGGGTACATCACCATGGCGT
  ATGTGTGGAACCGAGGGGATGACGATGTTCGAAAGGTGCTGCCCCGCAATATTGCTGTTCCCTACTGCGA
  GCTCTCAGAGAAGTTGGGCCTGCCTCCTATTCTGTCTTATGCAGACTGTGTCCTGGCAAACTGGAAGAAA
  AAGGACCCCAATGGGCCCATGACATACGAGAACATGGACATTCTGTTCTCATTTCCTGGTGGGGACTGCG
  ACAAGGGCTTCTTCCTCGTCTCTCTATTGGTGGAAATCGCAGCTTCTCCTGCAATCAAAGCAATCCCCAC
  TGTATCCAGTGCAGTAGAGCGTCAAGACCTGAAAGCATTGGAAAAGGCACTGCACGACATAGCTACCAGT
  CTGGAGAAAGCCAAGGAAATTTTTAAGAGGATGCGTGACTTTGTGGACCCAGACACGTTTTTCCACGTTC
  TCCGCATATATCTGTCTGGCTGGAAATGCAGCTCCAAGCTGCCAGAAGGTCTGCTGTATGAGGGGGTCTG
  GGACACCCCAAAAATGTTTTCAGGGGGCAGTGCAGGCCAGAGCAGCATCTTCCAGAGTCTTGATGTCCTT
  CTGGGAATAAAACACGAGGCTGGCAAAGAATCTCCTGCAGAATTCCTCCAGGAAATGAGAGAGTACATGC
  CTCCAGCCCACCGGAACTTCCTTTTCTTCTTAGAGTCAGCTCCCCCAGTCCGTGAGTTTGTCATTTCAAG
  ACACAATGAAGACTTGACGAAAGCTTATAACGAGTGTGTGAATGGTCTGGTCTCTGTGAGAAAGTTCCAC
  CTCGCAATAGTAGATACTTACATTATGAAACCTTCGAAGAAGAAGCCCACTGATGGCGACAAGTCGGAAG
  AGCCCTCAAATGTGGAAAGCAGAGGGACTGGGGGTACGAATCCCATGACTTTCCTAAGGAGTGTGAAAGA
  TACAACCGAGAAAGCTCTTCTGAGTTGGCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206394 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MALSKISPTEGSRRILEDHHIDEDVGFALPHPLVELPDAYSPWVLVARNLPVLIENGQLREEVEKLPTLS
  TDGLRGHRLQRLAHLALGYITMAYVWNRGDDDVRKVLPRNIAVPYCELSEKLGLPPILSYADCVLANWKK
  KDPNGPMTYENMDILFSFPGGDCDKGFFLVSLLVEIAASPAIKAIPTVSSAVERQDLKALEKALHDIATS
  LEKAKEIFKRMRDFVDPDTFFHVLRIYLSGWKCSSKLPEGLLYEGVWDTPKMFSGGSAGQSSIFQSLDVL
  LGIKHEAGKESPAEFLQEMREYMPPAHRNFLFFLESAPPVREFVISRHNEDLTKAYNECVNGLVSVRKFH
  LAIVDTYIMKPSKKKPTDGDKSEEPSNVESRGTGGTNPMTFLRSVKDTTEKALLSWP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008324
• ORF Size: 1224 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008324.2
• RefSeq Size: 1506 bp
• RefSeq ORF: 1224 bp
• Locus ID: 15930
• UniProt ID: P28776
• Cytogenetics: 8 A2
• MW: 45.6 kDa
• Gene Summary: Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. Acts as a suppressor of anti-tumor immunity (PubMed:25691885). Limits the growth of intracellular pathogens by depriving tryptophan. Protects the fetus from maternal immune rejection (Ref. 3).[UniProtKB/Swiss-Prot Function]