Lipa (NM_001111100) Mouse Tagged ORF Clone

CAT#: MR206231

  • TrueORF®

Lipa (Myc-DDK-tagged) - Mouse lysosomal acid lipase A (Lipa), transcript variant 2

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK


  "NM_001111100" in other vectors (2)


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Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Lipa
Synonyms AA960673; Lal; Lip-1; Lip1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR206231 representing NM_001111100
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAACTCCAGGGCCTGGTGTTCGTTTTCACCATTGGGATCCTGCTTTCTCGGGTGCCCACGGGAACTG
TATCAGCTGTGGATCCTGAAGTCAACATGAATGTGACCGAGATAATCATGCGCTGGGGATATCCTGGTGA
GGAACACTCGGTCCTGACAGGAGATGGCTATATTCTGAGTATTCACCGAATCCCTCGTGGGCGGAAGAAC
CATTTTGGCAAAGGTCCCAGACCAGTTGTGTATCTTCAGCACGGCTTGCTGGCAGATTCTAGTAACTGGG
TCACAAACATTGACAACAGCAGCTTGGGCTTCCTCCTGGCGGATGCTGGCTTTGATGTGTGGATGGGAAA
CAGCAGAGGAAACACCTGGTCTCTGAAGCACAAGACTCTTTCAGTTTCTCAGGATGAGTTCTGGGCCTTC
AGTTTTGATGAGATGGCAAAATATGACCTACCTGCTTCAATTAACTACATTTTGAATAAAACTGGCCAAG
AACAAATCTATTATGTGGGTCATTCTCAAGGCTGCACCATAGGTTTCATAGCTTTTTCACAGATGCCTGA
GTTGGCAAAGAAGATTAAAATGTTTTTAGTCTTGGCTCCCGTGTTGTCTCTCAATTTTGCTTCAGGCCCG
CTACTTCAATTGGGACGCTTGCCAGACCCTCTTCTCAAGGACATGTTTGGACAGAAGCAATTTCTCCCTC
AGAGTGCGATGCTGAAGTGGCTGAGCATTCACGTTTGCACCCACGTCATCATGAAGGAGCTTTGCGCAAA
CGTCTTCTTCCTGCTATGTGGCTTCAATGAGAAGAATTTAAATATGTCTAGAGTGGATGTGTATACAACA
CACTGCCCTGCGGGAACTTCTGTGCAAAACATGTTACACTGGGGTCAGGTTTTTAAATACCGTAAGCTTC
AAGCTTTTGACTGGGGAAGCAGTGAAAAGAATTATTTTCATTACAACCAGAGTTTTCCTCCCTCGTACAA
CATAAAAAACATGCGTCTACCCACTGCTTTGTGGAGCGGGGGCCGGGACTGGCTGGCAGACATCAATGAC
ATCACTATCTTACTGACTCAAATCCCCAAGTTGGTGTATCACAAGAACATTCCTGAGTGGGACCATCTGG
ATTTTATCTGGGGTTTGGATGCCCCCTGGAAACTGTATGATGAAATAATCAGTCTAATGAAGAAATACCA
G


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR206231 representing NM_001111100
Red=Cloning site Green=Tags(s)

MQLQGLVFVFTIGILLSRVPTGTVSAVDPEVNMNVTEIIMRWGYPGEEHSVLTGDGYILSIHRIPRGRKN
HFGKGPRPVVYLQHGLLADSSNWVTNIDNSSLGFLLADAGFDVWMGNSRGNTWSLKHKTLSVSQDEFWAF
SFDEMAKYDLPASINYILNKTGQEQIYYVGHSQGCTIGFIAFSQMPELAKKIKMFLVLAPVLSLNFASGP
LLQLGRLPDPLLKDMFGQKQFLPQSAMLKWLSIHVCTHVIMKELCANVFFLLCGFNEKNLNMSRVDVYTT
HCPAGTSVQNMLHWGQVFKYRKLQAFDWGSSEKNYFHYNQSFPPSYNIKNMRLPTALWSGGRDWLADIND
ITILLTQIPKLVYHKNIPEWDHLDFIWGLDAPWKLYDEIISLMKKYQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001111100
ORF Size 1191 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001111100.1, NP_001104570.1
RefSeq Size 2987 bp
RefSeq ORF 1194 bp
Locus ID 16889
UniProt ID Q9Z0M5
Cytogenetics 19 C1
MW 45.8 kDa
Gene Summary Crucial for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor-mediated endocytosis of lipoprotein particles. Important in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation (By similarity).[UniProtKB/Swiss-Prot Function]

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