Ercc8 (NM_028042) Mouse Tagged ORF Clone

CAT#: MR206229

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  • TrueORF®

Ercc8 (Myc-DDK-tagged) - Mouse excision repaiross-complementing rodent repair deficiency, complementation group 8 (Ercc8)



  "NM_028042" in other vectors (4)

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USD 300.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name Ercc8 (NM_028042) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Ercc8
Synonyms 2410022P04Rik; 2810431L23Rik; 4631412O06Rik; B130065P18Rik; Ckn1; Csa
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR206229 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGAGCTTTTTGTCCGCTCGACAGTCGGGTTTGGAGGACCCTCTTCGCTTGCGTAGAGCGCAGTCCA
CACGGAGGGTCCTGGGGCTGGAGTTAAACAAAGACAGGGATGTGGAAAGAATCCATGGCAGTGGGGTTAA
CACCCTCGACATTGAGCCCGTTGAAGGAAGATACATGTTGTCAGGTGGCTCCGATGGCGTGGTTGTACTC
TATGACCTTGAGAACGCCAGCAGACAGCCCCATTACACATGTAAAGCAGTGTGTTCCATTGGCAGAAGCC
ATCCCGATGTTCACAAATACAGCGTGGAGACTGTTCAGTGGTATCCTCATGACACTGGCATGTTCACATC
CAGCTCATTTGATAAAACTCTGAAAGTGTGGGATACAAACACATTGCAGGCTGCAGATGTGTTTAATTTT
GAGGAAACAGTTTACAGTCATCACATGTCCCCAGCAGCCACCAAGCACTGTCTGGTAGCAGTTGGAACAA
GAGGACCCAAGGTACAACTTTGTGACTTAAAGTCTGGATCCTGTTCTCACATTCTACAGGGTCACAGACA
GGAAATCTTGGCAGTTTCCTGGTCACCACGCCATGACTATATCTTGGCAACAGCAAGTGCTGACAGTAGA
GTAAAATTATGGGATGTGAGAAGAGCGTCAGGATGCTTGCTTACTCTTGACCAGCATAATGGGAAAAAGT
CACAAGCTGCTGAATCAGCAAACACTGCTCACAATGGGAAAGTTAACGGCTTATGTTTTACAAGTGACGG
CCTTCACCTGCTCACCATTGGCACAGACAACCGAATGCGCCTCTGGAATAGCTCCAGCGGGGACAACACC
CTGGTGAACTATGGGAAGGTGTGTAATGACAGCAGGAAAGGGCTGCAGTTCGCTGTCTCCTGTGGCTGCA
GCTCAGAGTTTGTGTTTGTTCCCCACGGCAGCACCATCGCTGTGTACGCAGTCCACTCGGGAGAGCGGCT
CGCCATGCTCAAGGGACATTACAAAAGCGTCGACTGCTGTGTGTTCCAGCCTAATTTCCAGGAACTTTAC
AGTGGAAGCAGGGACTGCAATATTCTTGCTTGGGTACCACCTTCCTATGAGCCAGTTCCTGATGATGACG
ATGAAGCTCCAGCCAAATCCCAGTTAAACCCAGCATTCGCGGACGCCTGGAGCAGCAGTGACGAGGATGG
G


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR206229 protein sequence
Red=Cloning site Green=Tags(s)

MLSFLSARQSGLEDPLRLRRAQSTRRVLGLELNKDRDVERIHGSGVNTLDIEPVEGRYMLSGGSDGVVVL
YDLENASRQPHYTCKAVCSIGRSHPDVHKYSVETVQWYPHDTGMFTSSSFDKTLKVWDTNTLQAADVFNF
EETVYSHHMSPAATKHCLVAVGTRGPKVQLCDLKSGSCSHILQGHRQEILAVSWSPRHDYILATASADSR
VKLWDVRRASGCLLTLDQHNGKKSQAAESANTAHNGKVNGLCFTSDGLHLLTIGTDNRMRLWNSSSGDNT
LVNYGKVCNDSRKGLQFAVSCGCSSEFVFVPHGSTIAVYAVHSGERLAMLKGHYKSVDCCVFQPNFQELY
SGSRDCNILAWVPPSYEPVPDDDDEAPAKSQLNPAFADAWSSSDEDG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_028042
ORF Size 1194 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_028042.1, NM_028042.2, NM_028042.3, NP_082318.2
RefSeq Size 2107 bp
RefSeq ORF 1194 bp
Locus ID 71991
Cytogenetics 13 D2.1
MW 43.7 kDa

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