Ercc8 (NM_028042) Mouse Tagged ORF Clone

CAT#: MR206229

Ercc8 (Myc-DDK-tagged) - Mouse excision repaiross-complementing rodent repair deficiency, complementation group 8 (Ercc8)

Product Images

Circular map for MR206229

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ercc8
• Synonyms: 2410022P04Rik; 2810431L23Rik; 4631412O06Rik; B130065P18Rik; Ckn1; Csa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR206229 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGAGCTTTTTGTCCGCTCGACAGTCGGGTTTGGAGGACCCTCTTCGCTTGCGTAGAGCGCAGTCCA
  CACGGAGGGTCCTGGGGCTGGAGTTAAACAAAGACAGGGATGTGGAAAGAATCCATGGCAGTGGGGTTAA
  CACCCTCGACATTGAGCCCGTTGAAGGAAGATACATGTTGTCAGGTGGCTCCGATGGCGTGGTTGTACTC
  TATGACCTTGAGAACGCCAGCAGACAGCCCCATTACACATGTAAAGCAGTGTGTTCCATTGGCAGAAGCC
  ATCCCGATGTTCACAAATACAGCGTGGAGACTGTTCAGTGGTATCCTCATGACACTGGCATGTTCACATC
  CAGCTCATTTGATAAAACTCTGAAAGTGTGGGATACAAACACATTGCAGGCTGCAGATGTGTTTAATTTT
  GAGGAAACAGTTTACAGTCATCACATGTCCCCAGCAGCCACCAAGCACTGTCTGGTAGCAGTTGGAACAA
  GAGGACCCAAGGTACAACTTTGTGACTTAAAGTCTGGATCCTGTTCTCACATTCTACAGGGTCACAGACA
  GGAAATCTTGGCAGTTTCCTGGTCACCACGCCATGACTATATCTTGGCAACAGCAAGTGCTGACAGTAGA
  GTAAAATTATGGGATGTGAGAAGAGCGTCAGGATGCTTGCTTACTCTTGACCAGCATAATGGGAAAAAGT
  CACAAGCTGCTGAATCAGCAAACACTGCTCACAATGGGAAAGTTAACGGCTTATGTTTTACAAGTGACGG
  CCTTCACCTGCTCACCATTGGCACAGACAACCGAATGCGCCTCTGGAATAGCTCCAGCGGGGACAACACC
  CTGGTGAACTATGGGAAGGTGTGTAATGACAGCAGGAAAGGGCTGCAGTTCGCTGTCTCCTGTGGCTGCA
  GCTCAGAGTTTGTGTTTGTTCCCCACGGCAGCACCATCGCTGTGTACGCAGTCCACTCGGGAGAGCGGCT
  CGCCATGCTCAAGGGACATTACAAAAGCGTCGACTGCTGTGTGTTCCAGCCTAATTTCCAGGAACTTTAC
  AGTGGAAGCAGGGACTGCAATATTCTTGCTTGGGTACCACCTTCCTATGAGCCAGTTCCTGATGATGACG
  ATGAAGCTCCAGCCAAATCCCAGTTAAACCCAGCATTCGCGGACGCCTGGAGCAGCAGTGACGAGGATGG
  G
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR206229 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLSFLSARQSGLEDPLRLRRAQSTRRVLGLELNKDRDVERIHGSGVNTLDIEPVEGRYMLSGGSDGVVVL
  YDLENASRQPHYTCKAVCSIGRSHPDVHKYSVETVQWYPHDTGMFTSSSFDKTLKVWDTNTLQAADVFNF
  EETVYSHHMSPAATKHCLVAVGTRGPKVQLCDLKSGSCSHILQGHRQEILAVSWSPRHDYILATASADSR
  VKLWDVRRASGCLLTLDQHNGKKSQAAESANTAHNGKVNGLCFTSDGLHLLTIGTDNRMRLWNSSSGDNT
  LVNYGKVCNDSRKGLQFAVSCGCSSEFVFVPHGSTIAVYAVHSGERLAMLKGHYKSVDCCVFQPNFQELY
  SGSRDCNILAWVPPSYEPVPDDDDEAPAKSQLNPAFADAWSSSDEDG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028042
• ORF Size: 1194 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028042.1, NM_028042.2, NM_028042.3, NP_082318.2
• RefSeq Size: 2107 bp
• RefSeq ORF: 1194 bp
• Locus ID: 71991
• UniProt ID: Q8CFD5
• Cytogenetics: 13 D2.1
• MW: 43.7 kDa
• Gene Summary: Involved in transcription-coupled nucleotide excision repair. It is required for the recruitment of XAB2, HMGN1 and TCEA1/TFIIS to a transcription-coupled repair complex which removes RNA polymerase II-blocking lesions from the transcribed strand of active genes. It is the substrate-recognition component of the CSA complex (DCX(ERCC8) complex) which promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair.[UniProtKB/Swiss-Prot Function]