Acta2 (NM_007392) Mouse Tagged ORF Clone
Acta2 (Myc-DDK-tagged) - Mouse actin, alpha 2, smooth muscle, aorta (Acta2)
|Product Name||Acta2 (NM_007392) Mouse Tagged ORF Clone|
|Type||Mouse Tagged ORF Clone|
|Synonyms||0610041G09Rik; a-SMA; Actvs; alphaSMA; SMAalpha; SMalphaA|
|E. coli Selection||Kanamycin (25 ug/mL)|
>MR205873 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>MR205873 protein sequence
Red=Cloning site Green=Tags(s)
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1134 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_007392.1, NM_007392.2, NM_007392.3, NP_031418.1|
|RefSeq Size||2572 bp|
|RefSeq ORF||1134 bp|
|Gene Summary||The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. [provided by RefSeq, Sep 2015]|
|The use of this cDNA Clones has been cited in the following citations:|
The role of a-smooth muscle actin in fibroblast-mediated matrix contraction and remodeling
,Shinde, AV;Humeres, C;Frangogiannis, NG;,
Biochim. Biophys. Acta 2016
,PubMed ID 27825850
|cDNA Clone Resources|
|MC204777||Acta2 (untagged) - Mouse actin, alpha 2, smooth muscle, aorta (Acta2), (10ug)||
|MG205873||Acta2 (GFP-tagged) - Mouse actin, alpha 2, smooth muscle, aorta (Acta2)||
|MR205873L1||Lenti ORF clone of Acta2 (Myc-DDK-tagged) - Mouse actin, alpha 2, smooth muscle, aorta (Acta2)||
|MR205873L2||Lenti ORF clone of Acta2 (mGFP-tagged) - Mouse actin, alpha 2, smooth muscle, aorta (Acta2)||
|MR205873L3||Lenti ORF clone of Acta2 (Myc-DDK-tagged) - Mouse actin, alpha 2, smooth muscle, aorta (Acta2)||
|MR205873L4||Lenti ORF clone of Acta2 (mGFP-tagged) - Mouse actin, alpha 2, smooth muscle, aorta (Acta2)||