Actc1 (BC062138) Mouse Tagged ORF Clone

CAT#: MR205866

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  • TrueORF®

Actc1 (Myc-DDK-tagged) - Mouse actin, alpha, cardiac (cDNA clone MGC:69991 IMAGE:6516862)



  "BC062138" in other vectors (4)

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USD 412.00


Availability*
3 Weeks

Size
    • 10 ug


Product images

Specifications

Product Data
Product Name Actc1 (BC062138) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Actc1
Synonyms Actc-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>MR205866 representing BC062138
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGTGACGATGAGGAGACCACCGCTTTGGTGTGTGACAATGGCTCCGGGCTGGTGAAGGCCGGCTTTG
CGGGTGATGACGCCCCCAGAGCTGTCTTCCCATCCATCGTGGGTCGCCCACGCCACCAGGGTGTCATGGT
GGGTATGGGACAGAAGGACTCCTATGTAGGTGATGAAGCCCAGAGCAAGCGAGGTATCCTGACTCTGAAG
TACCCCATTGAACATGGCATTATCACCAACTGGGATGACATGGAGAAGATCTGGCACCATACATTCTACA
ATGAGCTCCGTGTGGCCCCAGAGGAACACCCAACCCTGCTCACTGAAGCCCCGCTGAACCCCAAGGCCAA
CCGTGAGAAGATGACCCAGATCATGTTTGAAACCTTCAATGTGCCTGCCATGTATGTCGCCATCCAGGCA
GTGCTGTCTCTGTATGCTTCTGGAAGAACCACAGGCATTGTTCTGGATTCTGGCGATGGTGTAACTCATA
ATGTTCCCATCTATGAGGGTTATGCCTTACCCCATGCCATCATGCGCCTGGATCTAGCTGGTCGGGACCT
CACTGACTACCTCATGAAGATCCTCACTGAGCGCGGGTACTCCTTTGTCACCACTGCTGAACGTGAAATT
GTCCGTGACATAAAAGAAAAACTGTGTTACGTCGCCCTGGATTTTGAGAACGAGATGGCCACAGCTGCAT
CTTCCTCCTCTCTGGAGAAGAGCTATGAACTTCCTGACGGCCAGGTCATCACCATTGGCAACGAGCGCTT
CCGCTGTCCAGAGACTCTCTTCCAGCCCTCTTTCATTGGTATGGAATCTGCTGGCATTCATGAAACTACT
TACAACAGCATCATGAAGTGTGACATCGATATCCGCAAAGACCTGTATGCCAACAATGTCCTATCTGGAG
GCACCACCATGTACCCTGGTATTGCCGATCGTATGCAAAAGGAAATCACTGCACTGGCTCCCAGCACCAT
GAAGATCAAGATTATTGCTCCCCCTGAGCGTAAATACTCTGTCTGGATTGGGGGCTCCATCCTGGCCTCT
CTGTCCACCTTCCAGCAAATGTGGATCAGCAAGCAAGAGTATGATGAGGCAGGCCCATCCATTGTCCACC
GCAAGTGCTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR205866 representing BC062138
Red=Cloning site Green=Tags(s)

MCDDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLK
YPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQA
VLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREI
VRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETT
YNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILAS
LSTFQQMWISKQEYDEAGPSIVHRKCF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC062138
ORF Size 1131 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC062138.1
RefSeq Size 1454 bp
RefSeq ORF 1133 bp
Locus ID 11464
Cytogenetics 2 57.55 cM
MW 53.2 kDa
Gene Summary Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.[UniProtKB/Swiss-Prot Function]

Other Versions

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