St3gal3 (NM_001161774) Mouse Tagged ORF Clone

CAT#: MR205814

St3gal3 (Myc-DDK-tagged) - Mouse ST3 beta-galactoside alpha-2,3-sialyltransferase 3 (St3gal3), transcript variant 2

Product Images

Circular map for MR205814

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: St3gal3
• Synonyms: Siat3; Siat6; ST3GalIII; ST3N
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205814 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACTCTTGGTATTTGTGCGCAATCTGCTGCTAGCCCTCTGCCTCTTCCTGGTCCTGGGATTTTTGT
  ATTATTCTGCCTGGAAGCTACACTTACTCCAATGGGAAGACTCCAATTCATTGCTTCTTTCCCTTGACTC
  CGCTGGACAAACCCTAGGCACAGAGTATGATAGGCTGGGCTTCCTCCTGAAGCTGGACTCTAAACTGCCT
  GCCGAGCTAGCCACCAAGTACGCAAACTTTTCCGAGGGAGCTTGCAAACCCGGCTACGCTTCAGCTATGA
  TGACTGCCATCTTCCCCAGGTTCTCCAAGCCAGCACCCATGTTCCTGGATGACTCCTTTCGCAAGTGGGC
  TAGGATCCGGGAGTTTGTGCCACCTTTTGGGATCAAAGGTCAAGACAATCTGATCAAAGCCATCTTGTCA
  GTCACCAAAGAATACCGCCTGACCCCTGCCTTGGACAGCCTCCACTGCCGCCGCTGCATCATTGTAGGCA
  ATGGAGGGGTCCTCGCCAACAAGTCTCTGGGGTCACGGATTGACGACTATGACATTGTGATCAGACTGAA
  CTCAGCACCTGTGAAGGGCTTTGAGAGGGACGTGGGCAGCAAGACCACCCTACGCATCACCTACCCTGAG
  GGCGCCATGCAGCGACCTGAGCAATATGAACGAGACTCTCTCTTTGTCCTCGCTGGCTTCAAGTGGCAGG
  ACTTCAAGTGGCTGAAGTACATCGTCTACAAGGAGAGAGTGAGTGCATCTGATGGCTTCTGGAAGTCCGT
  GGCCACCCGAGTGCCCAAGGAGCCCCCTGAGATCCGCATCCTCAACCCATACTTCATCCAGGAGGCTGCC
  TTCACTCTCATTGGACTGCCCTTCAACAACGGCCTCATGGGCAGAGGGAACATCCCAACCCTTGGCAGTG
  TGGCAGTGACCATGGCACTACACGGCTGTGATGAAGTGGCAGTCGCGGGCTTTGGCTATGACATGAACAC
  ACCCAATGCACCCCTGCACTACTATGAAACTGTGCGCATGGCAGCCATCAAAGAGTCCTGGACACACAAC
  ATCCAGCGAGAGAAAGAGTTTCTGCGGAAGCTAGTGAAGGCACGTGTCATCACTGACTTAAGCAGCGGTA
  TC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205814 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGLLVFVRNLLLALCLFLVLGFLYYSAWKLHLLQWEDSNSLLLSLDSAGQTLGTEYDRLGFLLKLDSKLP
  AELATKYANFSEGACKPGYASAMMTAIFPRFSKPAPMFLDDSFRKWARIREFVPPFGIKGQDNLIKAILS
  VTKEYRLTPALDSLHCRRCIIVGNGGVLANKSLGSRIDDYDIVIRLNSAPVKGFERDVGSKTTLRITYPE
  GAMQRPEQYERDSLFVLAGFKWQDFKWLKYIVYKERVSASDGFWKSVATRVPKEPPEIRILNPYFIQEAA
  FTLIGLPFNNGLMGRGNIPTLGSVAVTMALHGCDEVAVAGFGYDMNTPNAPLHYYETVRMAAIKESWTHN
  IQREKEFLRKLVKARVITDLSSGI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161774
• ORF Size: 1125 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161774.1, NM_001161774.2, NP_001155246.1
• RefSeq Size: 2287 bp
• RefSeq ORF: 1125 bp
• Locus ID: 20441
• UniProt ID: P97325
• Cytogenetics: 4 D1- D2.1
• MW: 42.1 kDa
• Gene Summary: Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- and NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. The highest activity is toward Gal-beta-1,3-GlcNAc and the lowest toward Gal-beta-1,3-GalNAc.[UniProtKB/Swiss-Prot Function]