Aldoc (NM_009657) Mouse Tagged ORF Clone

CAT#: MR205567

Aldoc (Myc-DDK-tagged) - Mouse aldolase C, fructose-bisphosphate (Aldoc)

Product Images

Circular map for MR205567

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aldoc
• Synonyms: AI847350; Al; Aldo3; AU040929; Scr; Scrg2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR205567 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCACTCATACCCAGCTCTTTCTGCTGAGCAGAAGAAGGAGTTGTCGGATATTGCTCTACGGATCG
  TGGCCCCCGGCAAGGGCATTCTGGCTGCAGATGAGTCCGTAGGCAGCATGGCCAAAAGGCTGAGCCAAAT
  TGGGGTGGAGAACACTGAGGAGAATCGCCGGCTGTACCGCCAGGTCCTATTCAGTGCTGATGACCGTGTG
  AAAAAGTGCATTGGGGGGGTCATCTTCTTCCATGAGACACTCTACCAGAAAGATGACAATGGTGTCCCCT
  TCGTCCGCACCATCCAGGATAAGGGCATTCTCGTAGGCATCAAGGTTGACAAGGGTGTAGTGCCTCTAGC
  TGGGACCGACGGGGAAACCACCACTCAAGGGCTGGATGGGCTCTTGGAACGCTGTGCTCAGTATAAGAAG
  GACGGTGCTGATTTTGCCAAATGGCGCTGTGTACTAAAAATCAGTGATCGCACACCGTCGGCACTGGCCA
  TATTGGAGAATGCCAACGTGCTGGCCCGCTATGCCAGCATCTGCCAGCAGAATGGGATCGTGCCTATTGT
  GGAGCCTGAGATTCTGCCTGACGGAGACCATGACCTCAAACATTGCCAGTATGTTACAGAGAAGGTCCTG
  GCTGCTGTATACAAGGCCCTGAGTGACCATCATGTATACCTCGAAGGGACTCTGCTCAAGCCCAACATGG
  TGACCCCTGGCCATGCCTGTCCCATCAAGTATAGCCCAGAAGAGATTGCCATGGCAACTGTCACTGCCCT
  GCGTCGTACTGTGCCCCCAGCTGTCCCAGGGGTGACTTTCCTGTCTGGGGGTCAGAGTGAAGAGGAGGCT
  TCTCTCAACCTCAATGCCATCAACCGCTGCCCACTTCCCCGGCCCTGGGCCCTCACCTTCTCCTATGGGC
  GTGCCCTGCAGGCATCTGCACTCAATGCCTGGAGAGGACAAAGGGATAATGCTGGGGCTGCTACTGAGGA
  GTTTATCAAGCGGGCAGAGATGAACGGGCTTGCAGCCCAGGGCAGATATGAAGGCAGTGGAGATGGCGGA
  GCAGCAGCACAGTCCCTCTACGTCGCCAACCATGCCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR205567 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPHSYPALSAEQKKELSDIALRIVAPGKGILAADESVGSMAKRLSQIGVENTEENRRLYRQVLFSADDRV
  KKCIGGVIFFHETLYQKDDNGVPFVRTIQDKGILVGIKVDKGVVPLAGTDGETTTQGLDGLLERCAQYKK
  DGADFAKWRCVLKISDRTPSALAILENANVLARYASICQQNGIVPIVEPEILPDGDHDLKHCQYVTEKVL
  AAVYKALSDHHVYLEGTLLKPNMVTPGHACPIKYSPEEIAMATVTALRRTVPPAVPGVTFLSGGQSEEEA
  SLNLNAINRCPLPRPWALTFSYGRALQASALNAWRGQRDNAGAATEEFIKRAEMNGLAAQGRYEGSGDGG
  AAAQSLYVANHAY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009657
• ORF Size: 1092 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009657.1
• RefSeq Size: 1702 bp
• RefSeq ORF: 1092 bp
• Locus ID: 11676
• UniProt ID: P05063
• Cytogenetics: 11 46.74 cM
• MW: 39.3 kDa
• Gene Summary: This gene encodes a member of the aldolase family of enzymes that is mainly expressed in neuronal tissues. The encoded protein is an enzyme of the glycolysis pathway, and catalyzes the conversion of fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2014]