Aurkb (NM_011496) Mouse Tagged ORF Clone

CAT#: MR205201

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  • TrueORF®

Aurkb (Myc-DDK-tagged) - Mouse aurora kinase B (Aurkb)



  "NM_011496" in other vectors (4)

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Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 340.00


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Size
    • 10 ug


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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Aurkb
Synonyms AI; Aik2; AIM-1; Aim1; AIRK2; AL022959; Ark2; AurB; I; IPL1; Stk; STK-; STK-1; Stk1; Stk5; Stk12
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR205201 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCAGAAGGAGAACGCCTACCCGTGGCCCTACGGCTCAAAGACGTCTCAGTCTGGCCTGAACACGT
TGTCCCAGAGAGTCCTACGGAAGGAGCCCGCCACGACATCTGCGCTTGCTCTCGTGAACCGGTCCAACAG
CCAGTCCACAGCTGCCCCTGGCCAGAAGTTGGCTGAGAACAAGAGTCAGGGCTCCACTGCCTCGCAAGGA
TCCCAGAACAAGCAGCCTTTCACTATTGACAACTTTGAGATTGGGCGTCCTTTGGGCAAAGGCAAATTTG
GAAACGTGTACTTGGCTCGGGAGAAGAAGAGCCGTTTCATCGTGGCACTCAAGATCCTCTTCAAGTCTCA
GATTGAGAAGGAGGGGGTAGAGCACCAGCTTCGCCGAGAGATCGAAATCCAGGCGCACCTGAAACATCCC
AACATCCTTCAACTCTACAACTACTTCTACGACCAGCAGAGGATCTACTTAATCCTGGAATACGCCCCTC
GCGGGGAACTCTACAAGGAACTGCAGAAGAGTCGGACCTTCGATGAGCAGCGGACTGCCACGATCATGGA
GGAACTGTCAGATGCCCTGACCTACTGCCACAAGAAGAAGGTAATTCACAGAGACATAAAGCCGGAGAAC
CTGCTGTTAGGTCTGCAGGGAGAACTGAAGATTGCAGACTTTGGCTGGTCGGTGCATGCCCCATCCCTGA
GGAGGAAGACCATGTGCGGCACGCTGGACTATCTGCCCCCAGAGATGATTGAGGGGCGCATGCATAATGA
AATGGTAGATCTATGGTGCATCGGGGTGCTCTGCTATGAACTGATGGTGGGGAACCCACCCTTCGAGAGC
CCTAGCCACAGTGAGACGTATCGTCGGATTGTCAAGGTGGACCTGAAGTTCCCCTCTTCTGTGCCTTCGG
GCGCCCAGGACCTCATCTCCAAGCTGCTCAAACATAACCCCTGGCAACGGCTGCCCCTGGCGGAGGTTGC
AGCTCACCCTTGGGTCCGGGCCAACTCAAGGAGGGTTCTGCCTCCCTCTGCCCTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR205201 protein sequence
Red=Cloning site Green=Tags(s)

MAQKENAYPWPYGSKTSQSGLNTLSQRVLRKEPATTSALALVNRSNSQSTAAPGQKLAENKSQGSTASQG
SQNKQPFTIDNFEIGRPLGKGKFGNVYLAREKKSRFIVALKILFKSQIEKEGVEHQLRREIEIQAHLKHP
NILQLYNYFYDQQRIYLILEYAPRGELYKELQKSRTFDEQRTATIMEELSDALTYCHKKKVIHRDIKPEN
LLLGLQGELKIADFGWSVHAPSLRRKTMCGTLDYLPPEMIEGRMHNEMVDLWCIGVLCYELMVGNPPFES
PSHSETYRRIVKVDLKFPSSVPSGAQDLISKLLKHNPWQRLPLAEVAAHPWVRANSRRVLPPSAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_011496
ORF Size 1038 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_011496.1, NM_011496.2, NP_035626.1
RefSeq Size 1957 bp
RefSeq ORF 1038 bp
Locus ID 20877
UniProt ID O70126
Cytogenetics 11 42.32 cM
MW 39.3 kDa
Gene Summary This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 2 and 7. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. [provided by RefSeq, Sep 2015]

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