Wdr5 (NM_080848) Mouse Tagged ORF Clone
CAT#: MR204938
- TrueORF®
Wdr5 (Myc-DDK-tagged) - Mouse WD repeat domain 5 (Wdr5)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_080848" in other vectors (4)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Wdr5 |
Synonyms | 2410008O07Rik; AA408785; AA960360; Big; Big-3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR204938 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACAGAGGAGAAGAAGCCAGAGACAGAGGCTGCAAGAGCACAGCCCACTCCTTCCTCATCAGCCA CACAGAGCAAGCCCACACCAGTTAAGCCAAACTATGCCCTGAAGTTCACCCTGGCTGGCCACACCAAAGC TGTGTCCTCTGTGAAGTTCAGCCCCAATGGGGAATGGTTGGCAAGTTCATCTGCTGATAAACTCATTAAA ATTTGGGGAGCATATGATGGAAAGTTTGAGAAAACTATATCTGGTCACAAACTGGGAATATCTGATGTAG CGTGGTCATCAGATTCTAACCTCCTTGTGTCTGCCTCTGATGATAAAACTTTGAAGATTTGGGACGTGAG TTCCGGGAAGTGTCTGAAGACCCTGAAGGGCCACAGTAACTACGTCTTCTGCTGCAACTTCAACCCCCAG TCCAACCTCATCGTCTCAGGGTCTTTTGATGAAAGTGTGAGGATATGGGACGTGAAGACAGGGAAGTGCC TCAAGACTTTGCCTGCCCATTCGGACCCAGTCTCAGCCGTTCATTTCAACCGTGATGGATCATTGATTGT TTCCAGTAGCTATGATGGCCTCTGCCGAATCTGGGACACCGCCTCTGGCCAGTGTCTGAAGACACTCATT GATGATGACAATCCTCCAGTGTCCTTCGTGAAGTTCTCTCCAAATGGCAAATACATCCTGGCTGCAACTT TGGACAACACACTGAAGCTCTGGGACTACAGCAAGGGGAAGTGCCTGAAGACATACACTGGCCACAAGAA TGAGAAGTACTGCATATTTGCCAACTTCTCCGTGACAGGCGGGAAGTGGATTGTGTCTGGTTCTGAAGAT AACCTGGTGTATATCTGGAATCTGCAGACCAAGGAGATTGTGCAGAAGTTGCAGGGTCACACAGATGTTG TGATTTCCACGGCTTGTCACCCGACAGAGAACATCATTGCCTCAGCAGCGTTAGAGAACGACAAAACAAT CAAACTGTGGAAGAGTGACTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204938 protein sequence
Red=Cloning site Green=Tags(s) MATEEKKPETEAARAQPTPSSSATQSKPTPVKPNYALKFTLAGHTKAVSSVKFSPNGEWLASSSADKLIK IWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGKCLKTLKGHSNYVFCCNFNPQ SNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLI DDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSED NLVYIWNLQTKEIVQKLQGHTDVVISTACHPTENIIASAALENDKTIKLWKSDC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_080848 |
ORF Size | 1005 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_080848.2 |
RefSeq Size | 2916 bp |
RefSeq ORF | 1005 bp |
Locus ID | 140858 |
UniProt ID | P61965 |
Cytogenetics | 2 A3 |
MW | 36.6 kDa |
Gene Summary | Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC201956 | Wdr5 (untagged) - Mouse WD repeat domain 5 (Wdr5), (10ug) |
USD 457.00 |
|
MG204938 | Wdr5 (tGFP-tagged) - Mouse WD repeat domain 5 (Wdr5) |
USD 657.00 |
|
MR204938L3 | Lenti ORF clone of Wdr5 (Myc-DDK-tagged) - Mouse WD repeat domain 5 (Wdr5) |
USD 757.00 |
|
MR204938L4 | Lenti ORF clone of Wdr5 (mGFP-tagged) - Mouse WD repeat domain 5 (Wdr5) |
USD 757.00 |
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