Akr1b8 (NM_008012) Mouse Tagged ORF Clone

CAT#: MR204542

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  • TrueORF®

Akr1b8 (Myc-DDK-tagged) - Mouse aldo-keto reductase family 1, member B8 (Akr1b8)



  "NM_008012" in other vectors (4)

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Reconstitution Protocol

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USD 305.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Akr1b8
Synonyms Fgfrp; Fgrp; FR-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR204542 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACGTTCGTGGAACTCAGTACCAAAGCCAAGATGCCCATTGTGGGCCTGGGCACCTGGAAGTCTC
CCCCAAACCAAGTCAAAGAAGCTGTGAAGGCGGCCATTGACGCTGGGTATCGCCATATCGACTGCGCGTA
TGCCTATTGCAACGAGAATGAGGTGGGAGAAGCCATCCAAGAGAAGATCAAAGAGAAGGCTGTGCAGCGG
GAGGACCTCTTCATTGTCAGCAAGCTGTGGCCCACCTGCTTTGAGAAGAAACTGCTAAAGGAAGCCTTTC
AGAAGACCCTCACGGATCTGAAGCTGGACTATTTGGACCTCTATCTTATTCACTGGCCACAGGGACTTCA
GCCAGGAAAGGAGTTATTCCCCAAAGATGACCAAGGCAGAATCCTCACCAGTAAGACAACATTCTTGGAA
GCCTGGGAGGGCATGGAGGAACTGGTGGACCAGGGGCTGGTGAAAGCTCTGGGCGTCTCCAACTTCAACC
ACTTCCAGATTGAAAGGCTCCTGAACAAGCCTGGACTAAAACATAAGCCAGTGACCAACCAGGTTGAGTG
TCATCCTTACCTCACCCAGGAAAAACTGATCCAGTACTGTCACTCGAAGGGCATCTCTGTCACTGCCTAC
AGTCCCCTGGGCTCCCCAGACAGGCCTAGCGCCAAGCCAGAGGACCCTTCACTATTAGAGGACCCCAAAA
TTAAAGAGATTGCCGCCAAGCACAAGAAAACCTCAGCCCAGGTTCTGATTCGGTTTCACATCCAGAGGAA
CGTGGTGGTGATCCCGAAGTCTGTGACGCCATCACGTATACAGGAGAACATTCAGGTCTTTGATTTCCAG
TTGAGTGACGAGGAGATGGCCACTATCCTTAGCTTCAACAGAAACTGGAGGGCCTGCCTGCTGCCTGAGA
CAGTAAACATGGAAGAATATCCCTATGATGCAGAATAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR204542 protein sequence
Red=Cloning site Green=Tags(s)

MATFVELSTKAKMPIVGLGTWKSPPNQVKEAVKAAIDAGYRHIDCAYAYCNENEVGEAIQEKIKEKAVQR
EDLFIVSKLWPTCFEKKLLKEAFQKTLTDLKLDYLDLYLIHWPQGLQPGKELFPKDDQGRILTSKTTFLE
AWEGMEELVDQGLVKALGVSNFNHFQIERLLNKPGLKHKPVTNQVECHPYLTQEKLIQYCHSKGISVTAY
SPLGSPDRPSAKPEDPSLLEDPKIKEIAAKHKKTSAQVLIRFHIQRNVVVIPKSVTPSRIQENIQVFDFQ
LSDEEMATILSFNRNWRACLLPETVNMEEYPYDAEY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_008012
ORF Size 951 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_008012.1, NP_032038.1
RefSeq Size 1304 bp
RefSeq ORF 951 bp
Locus ID 14187
UniProt ID P45377
Cytogenetics 6 14.91 cM
MW 36.1 kDa

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