Cnksr3 (BC031194) Mouse Tagged ORF Clone

CAT#: MR204484

Cnksr3 (Myc-DDK-tagged) - Mouse Cnksr family member 3 (cDNA clone MGC:37681 IMAGE:5059479)

Product Images

Circular map for MR204484

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cnksr3
• Synonyms: MGC37681, 6820402C05
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204484 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTCTCCTGCAGACAGATCTCAAAAGATTCACGCTGGCGATGAGGTCATTCAAGTCAATCGGCAGA
  CAGTGGTTGGGTGGCAGTTGAAAAATCTGGTGAGAAAGTTGAGAGAGAACCCCACGGGCGTGGTGCTGCT
  GCTGAAGAAGAGGCCCACGGGCTCCTTCAGCTTCACTCCGGCCCCACTGAAGAACCTGCGGTGGAAGCCA
  CCGCTCGTGCAGACCTCACCCCCGCCCACCACCACCCAGTCCCCAGAGAGTACCATGGATGCCTCCCTGA
  AGAAGGAGAAGCCAGCCATCCTGGACCTATACATCCCTCCCCCACCTGCTGTCCCCTACTCTCCCCGGGA
  TGAGAATGTGAGTTTCGGCTACAGAGGACACAGTAAGAGTAAGCAGCCGTTGCCTGTGCGCAAGGGTTCC
  GAGTCCCCTAACTCCTTCCTGGACCAGGAGAGCCAAAGACGCAGATTTACCATTGCAGATTCCGATCAGC
  TGCCCGGGTATTCGGTGGAAACCAATGTTCTGCCCACAAAAATGAGAGGGAAGACACCATCCTACGGCAA
  ACCCCGGCCTCTGTCCATGCCTGCCGATGGGAACTGGATGGGGATCGTGGACCCTTTTGCTAAACCTCGA
  GGAAATGGGAGGAAAGGGGAAGATGCCCTCTGCCGGTATTTCAGCAATGAGCGGATCACACCCATCACTG
  AAGAAAGTGCCTCTCCTATGTACCGGTTCTCTAGACCCCTGACTGAGCGGCACCTGGTCCGAGGTGCGGA
  CTACATCCGAGGGAGCAGGTGCTACATCAACTCCGACCTACACAGCAGTGCCACCATTCCATTCCAGGAG
  GAAGGTTCCAAGAAGAAATCAGCCTCCTCATCGGCCAAGGCGTCCTCTGGGGAGCCGTCGCTGCTGGTCA
  GCTGGCTGACTCGCCTCAAGCTGTTGACTCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204484 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQSPADRSQKIHAGDEVIQVNRQTVVGWQLKNLVRKLRENPTGVVLLLKKRPTGSFSFTPAPLKNLRWKP
  PLVQTSPPPTTTQSPESTMDASLKKEKPAILDLYIPPPPAVPYSPRDENVSFGYRGHSKSKQPLPVRKGS
  ESPNSFLDQESQRRRFTIADSDQLPGYSVETNVLPTKMRGKTPSYGKPRPLSMPADGNWMGIVDPFAKPR
  GNGRKGEDALCRYFSNERITPITEESASPMYRFSRPLTERHLVRGADYIRGSRCYINSDLHSSATIPFQE
  EGSKKKSASSSAKASSGEPSLLVSWLTRLKLLTH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC031194
• ORF Size: 942 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031194, AAH31194
• RefSeq Size: 2566 bp
• RefSeq ORF: 944 bp
• Locus ID: 215748
• Cytogenetics: 10 A1
• MW: 35 kDa
• Gene Summary: Involved in transepithelial sodium transport. Regulates aldosterone-induced and epithelial sodium channel (ENaC)-mediated sodium transport through regulation of ENaC cell surface expression. Acts as a scaffold protein coordinating the assembly of an ENaC-regulatory complex (ERC).[UniProtKB/Swiss-Prot Function]