Ufd1 (NM_011672) Mouse Tagged ORF Clone
CAT#: MR204334
- TrueORF®
Ufd1l (Myc-DDK-tagged) - Mouse ubiquitin fusion degradation 1 like (Ufd1l), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_011672" in other vectors (4)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Ufd1 |
Synonyms | Ufd1; Ufd1l |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR204334 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTTCTTTCAACATGTTTGACCACCCGATTCCCCGGGTCTTCCAGAACCGCTTCTCCACGCAGTACC GCTGCTTCTCCGTGTCCATGCTAGCAGGGCCTAATGACAGGTCAGATGTGGAGAAAGGAGGGAAGATAAT TATGCCACCCTCAGCCCTCGATCAACTCAGCCGGCTCAACATTACCTATCCTATGCTGTTTAAATTGACC AATAAGAATTCAGATCGGATGACACACTGCGGTGTACTGGAGTTTGTTGCTGATGAAGGCATCTGTTACC TCCCCCACTGGATGATGCAGAATTTGCTGTTGGAGGAAGGGGGCCTGGTTCAGGTGGAAAGTGTCAACCT CCAAGTGGCGACCTACTCTAAGTTCCAGCCTCAGAGCCCAGACTTCCTGGATATTACCAACCCTAAAGCG GTATTAGAAAATGCATTGAGAAACTTCGCCTGTCTGACGACTGGAGATGTGATTGCTATCAACTACAATG AGAAGATCTATGAGCTGCGGGTGATGGAGACCAAACCTGACAAGGCTGTATCCATTATTGAATGTGACAT GAATGTGGATTTTGATGCTCCCTTGGGATACAAAGAACCAGAAAGACCAGTGCAGCATGAGGAGTCAATA GAGGGAGAAGCTGACCACAGTGGCTATGCCGGAGAGGTGGGCTTCCGTGCCTTCTCTGGTTCTGGGAATA GACTGGATGGGAAGAAAAAAGGGGTTGAGCCCAGTCCCTCCCCAATCAAGCCTGGAGACATCAAAAGAGG AATTCCTAATTACGAATTTAAGCTTGGTAAGATCACTTTCATCAGAAATTCACGTCCATTGGTCAAAAAG GTTGAAGAGGATGAAGCTGGAGGCAGATTCATTGCTTTCTCTGGAGAAGGACAGTCACTGCGTAAGAAGG GAAGAAAGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR204334 protein sequence
Red=Cloning site Green=Tags(s) MFSFNMFDHPIPRVFQNRFSTQYRCFSVSMLAGPNDRSDVEKGGKIIMPPSALDQLSRLNITYPMLFKLT NKNSDRMTHCGVLEFVADEGICYLPHWMMQNLLLEEGGLVQVESVNLQVATYSKFQPQSPDFLDITNPKA VLENALRNFACLTTGDVIAINYNEKIYELRVMETKPDKAVSIIECDMNVDFDAPLGYKEPERPVQHEESI EGEADHSGYAGEVGFRAFSGSGNRLDGKKKGVEPSPSPIKPGDIKRGIPNYEFKLGKITFIRNSRPLVKK VEEDEAGGRFIAFSGEGQSLRKKGRKP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_011672 |
ORF Size | 924 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_011672.4, NP_035802.3 |
RefSeq Size | 1996 bp |
RefSeq ORF | 924 bp |
Locus ID | 22230 |
UniProt ID | P70362 |
Cytogenetics | 16 11.65 cM |
MW | 34.5 kDa |
Gene Summary | Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. It may be involved in the development of some ectoderm-derived structures (By similarity). Acts as a negative regulator of type I interferon production via the complex formed with VCP and NPLOC4, which binds to DDX58/RIG-I and recruits RNF125 to promote ubiquitination and degradation of DDX58/RIG-I (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208050 | Ufd1l (untagged) - Mouse ubiquitin fusion degradation 1 like (Ufd1l), transcript variant 1, (10ug) |
USD 330.00 |
|
MG204334 | Ufd1l (tGFP-tagged) - Mouse ubiquitin fusion degradation 1 like (Ufd1l) |
USD 500.00 |
|
MR204334L3 | Lenti ORF clone of Ufd1l (Myc-DDK-tagged) - Mouse ubiquitin fusion degradation 1 like (Ufd1l), transcript variant 1 |
USD 600.00 |
|
MR204334L4 | Lenti ORF clone of Ufd1l (mGFP-tagged) - Mouse ubiquitin fusion degradation 1 like (Ufd1l), transcript variant 1 |
USD 600.00 |
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