Ankrd54 (NM_144849) Mouse Tagged ORF Clone

CAT#: MR204172

Ankrd54 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 54 (Ankrd54)

Product Images

Circular map for MR204172

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ankrd54
• Synonyms: C730048E16Rik; EST475269; EST1068184; Liar
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204172 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCACCGGCGGGGGCGCGGACGACGAGTCGCGATCCGGCCGCTCGAGCTCGGACGGCGAGTGCG
  CGGTGGCGCCGGAGCCTTTGGCGGAAGCCGGAGGCCTGGTGTCTTTCGCGGACTTCGGCGTTTCGCTGGG
  CAGCGGCGCCGGCCTCCCGGGCCGGTCGGTGGGCAGGGCCCAGTCCTCGCTGCGCTACCTCCAGGTCCTG
  TGGCAGCAGGACGTCGAGCCTCGCGACGAACTGCGTTGCAAGATTCCCGCCGGGCGGTTGAGGCGTGCCG
  CCAGGCCCCACCGTCGGCTCGGGCCCACCGGCAAGGAGGTACACGCTCTGAAGAGGCTGAGGGACTCTGC
  CAATGCCAACGACGTAGAAACAGTGCAGCAGCTGCTGGAAGATGGGGCGGATCCCTGCGCAGCGGATGAT
  AAAGGCAGAACTGCCCTCCATTTTGCTTCCTGCAATGGCAATGACCAGATTGTGCAGCTGCTCCTGGACC
  ATGGGGCTGACCCCAACCAACAGGATGGTCTGGGCAACACGCCATTGCACCTGGCGGCCTGCACCAACCA
  TGTTCCTGTCATCACCACACTGCTTCGAGGAGGGGCCCGTGTAGATGCCCTAGACAGAGCTGGCCGCACA
  CCCCTACACCTGGCCAAGTCGAAGCTGAACATCCTACAGGAGGGTCATTCCCAGTGTCTGGAGGCCGTCC
  GCCTGGAGGTGAAGCAGATCATCCACATGCTACGCGAGTACCTGGAGCGCCTGGGGCGGCATGAGCAGCG
  AGAACGGCTAGATGACCTCTGCACCCGCCTCCAGATGACAAGTACCAAAGAACAGGTGGATGAAGTGACG
  GACCTCTTGGCCAGCTTCACCTCCCTCAGTCTGCAGATGCAGAGTATGGAGAAGAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204172 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAATGGGADDESRSGRSSSDGECAVAPEPLAEAGGLVSFADFGVSLGSGAGLPGRSVGRAQSSLRYLQVL
  WQQDVEPRDELRCKIPAGRLRRAARPHRRLGPTGKEVHALKRLRDSANANDVETVQQLLEDGADPCAADD
  KGRTALHFASCNGNDQIVQLLLDHGADPNQQDGLGNTPLHLAACTNHVPVITTLLRGGARVDALDRAGRT
  PLHLAKSKLNILQEGHSQCLEAVRLEVKQIIHMLREYLERLGRHEQRERLDDLCTRLQMTSTKEQVDEVT
  DLLASFTSLSLQMQSMEKR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144849
• ORF Size: 900 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144849.1, NP_659098.1
• RefSeq Size: 1894 bp
• RefSeq ORF: 900 bp
• Locus ID: 223690
• UniProt ID: Q91WK7
• Cytogenetics: 15 37.7 cM
• MW: 32.5 kDa
• Gene Summary: Plays an important role in regulating intracellular signaling events associated with erythroid terminal differentiation.[UniProtKB/Swiss-Prot Function]