Mgat4b (BC031613) Mouse Tagged ORF Clone

CAT#: MR204088

  • TrueORF®

Mgat4b (Myc-DDK-tagged) - Mouse mannoside acetylglucosaminyltransferase 4, isoenzyme B (cDNA clone MGC:36850 IMAGE:4216720)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK


  "BC031613" in other vectors (4)


Interest in protein/lysate? Submit request here!

Reconstitution Protocol

USD 300.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

USD 198.00

Other products for "Mgat4b"

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Mgat4b
Synonyms AA407995; GnTIVb
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR204088 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGTATGCACAGTCCAAAGGCATCTACTATGTGCAGCTGGAGGATGACATTGTAGCCAAGCCCAACT
ACTTGAGCACTATGAAGAACTTTGCCCTCCAGCAGCCCTCCGAGGACTGGATGATCCTGGAGTTCTCGCA
GTTGGGCTTCATTGGGAAGATGTTCAAGTCACTGGATCTGAGCCTGATTGTGGAGTTCATCCTCATGTTC
TACCGGGACAAGCCCATAGACTGGCTCCTGGACCACATCCTGTGGGTGAAAGTCTGCAACCCTGAGAAGG
ATGCGAAACATTGTGATCGGCAGAAGGCCAACCTTCGGATCCGCTTCAAGCCGTCCCTTTTCCAGCATGT
GGGCACTCACTCATCACTGGCGGGCAAAATCCAGAAACTGAAGGATAAAGACTTTGGAAAGCATGCTCTC
CGGAAGGAGCACGTGAACCCACCGGCAGAGGTGAGCACAAGCCTCAAGACGTACCAGCATTTCACCCTGG
AGAAGGCCTACTTGCGGGAGGATTTCTTCTGGGCCTTCACACCTGCCGCAGGAGACTTTATCCGGTTCCG
CTTCTTCCAGCCACTGCGCCTTGAGCGGTTCTTCTTCCGAAGCGGGAACATCGAGCACCCGGAAGATAAG
CTCTTCAACACTTCTGTGGAGGTGCTGCCCTTTGATAACCCCCAGTCAGAGAAGGAGGCCCTTCAGGAAG
GCCGCTCAGCCACTCTCCGGTACCCTAGGAGCCCAGATGGATACCTCCAGATTGGCTCCTTCTACAAGGG
TGTAGCTGAAGGAGAAGTGGATCCTGCCTTTGGCCCCCTGGAAGCACTACGTCTCTCCATTCAGACTGAC
TCCCCGGTGTGGGTCATTTTGAGTGAGATCTTTCTGAAAAAGGCCGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR204088 protein sequence
Red=Cloning site Green=Tags(s)

MMYAQSKGIYYVQLEDDIVAKPNYLSTMKNFALQQPSEDWMILEFSQLGFIGKMFKSLDLSLIVEFILMF
YRDKPIDWLLDHILWVKVCNPEKDAKHCDRQKANLRIRFKPSLFQHVGTHSSLAGKIQKLKDKDFGKHAL
RKEHVNPPAEVSTSLKTYQHFTLEKAYLREDFFWAFTPAAGDFIRFRFFQPLRLERFFFRSGNIEHPEDK
LFNTSVEVLPFDNPQSEKEALQEGRSATLRYPRSPDGYLQIGSFYKGVAEGEVDPAFGPLEALRLSIQTD
SPVWVILSEIFLKKAD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC031613
ORF Size 888 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC031613, AAH31613
RefSeq Size 1426 bp
RefSeq ORF 890 bp
Locus ID 103534
Cytogenetics 11 B1.3
MW 34.4 kDa
Gene Summary Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Has lower affinities for donors or acceptors than MGAT4A, suggesting that, under physiological conditions, it is not the main contributor in N-glycan biosynthesis (By similarity).[UniProtKB/Swiss-Prot Function]

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Order online and get additional $20 off!

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.