Nrbf2 (NM_001036293) Mouse Tagged ORF Clone

CAT#: MR203903

Nrbf2 (Myc-DDK-tagged) - Mouse nuclear receptor binding factor 2 (Nrbf2)

Product Images

Circular map for MR203903

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nrbf2
• Synonyms: NRBF-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203903 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGTGATGGAAGGACCCCTCAACCTTGCTCACCAGCAGAGCCGCCGAGCAGACCGTTTGTTAGCTG
  CTGGGAAATACGAAGAGGCGATTTCTTGTCACAGAAAGGCAACAACATATCTCTCTGAAGCCATGAAGCT
  GACCGAGTCTGAGCAGGCTCACTTATCACTGGAACTGCAAAGGGACAGCCACATGAAACAGCTGCTTCTC
  ATCCAGGAGAGGTGGAAGAGAGCAAAGCGAGAGGAGCGGTTGAAAGCGCAGCAGAGCACGGAACGGGACG
  GAGCCCCCCACCTGCAGGCACCTCCCAGGCCCTCTGAGGATGCTGAAGGCCAGAGCCCTCTGCTTTCTCA
  GCCATACATCCCTTCCACAGAGAGGCGCCTCCCCGAGGTGCAGGGGGTCTTTGACAGGGATCCAGACACA
  TTGCTGTTTTTACTTCAGCAAAAGAATGAGCCATCAGAGCCCTGTATTGGAAGCAAAGCCCCAAAAGATG
  ATAAAACGATTATAGAGGAGCAGGCAACCAAAATAGCAGATTTGAAGAGGCATGTGGAGTTCCTTGTGGC
  TGAGAATGAAAGATTAAGGAAAGAAAATAAACAGCTAAAGGCTGAAAAGGCCAGACTCCTGAAAGGCACA
  GCTGAGAAGGAACTGGACGTCGATGCAGACTTTGTAGAGAAGTCAGAGCTGTGGGGCTTACCTTCACACT
  CGGAATCCGCCGCTGCCTCTTCAACCTGGCAGAAGTTTGCAGCTAACACGGGGAAAGCCAAGGACATTCC
  AATACCCAACCTTCCTCCTCTGGATTTCCCATCTCCAGAGCTTCCTCTGATGGAGCTCTCGGAGGACATT
  CTGAAAGGATTCATGAATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203903 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEVMEGPLNLAHQQSRRADRLLAAGKYEEAISCHRKATTYLSEAMKLTESEQAHLSLELQRDSHMKQLLL
  IQERWKRAKREERLKAQQSTERDGAPHLQAPPRPSEDAEGQSPLLSQPYIPSTERRLPEVQGVFDRDPDT
  LLFLLQQKNEPSEPCIGSKAPKDDKTIIEEQATKIADLKRHVEFLVAENERLRKENKQLKAEKARLLKGT
  AEKELDVDADFVEKSELWGLPSHSESAAASSTWQKFAANTGKAKDIPIPNLPPLDFPSPELPLMELSEDI
  LKGFMND
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001036293
• ORF Size: 864 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001036293.2, NP_001031370.1
• RefSeq Size: 1789 bp
• RefSeq ORF: 864 bp
• Locus ID: 641340
• UniProt ID: Q8VCQ3
• Cytogenetics: 10 B5.1
• MW: 32.5 kDa
• Gene Summary: May modulate transcriptional activation by target nuclear receptors. Can act as transcriptional activator (in vitro) (By similarity).[UniProtKB/Swiss-Prot Function]