Ssr1 (NM_025965) Mouse Tagged ORF Clone

CAT#: MR203901

Ssr1 (Myc-DDK-tagged) - Mouse signal sequence receptor, alpha (Ssr1)

Product Images

Circular map for MR203901

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ssr1
• Synonyms: 2510001K09Rik; 6330400D04; AI159733; AI452176; SSR; TRAPA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203901 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACTCCTCCCCCGCCTGCTGCTGCTGTTCCTGCTGGCCTTCCCCGCCGCCGTGCTGCTCCGAGGCG
  GCCCCGGAGGGTCATTAGCTTTGGCTCAAGATCCTACAGAAGATGAAGAAATAGTAGAAGATTCAATAAT
  TGAAGATGAAGATGATGAAGCTGAAGTAGAAGAAGATGAACCCACTGATTTGGCAGAAGATAAAGAAGAG
  GAGGATGTATCCAGTGAACCAGAAGCTTCACCCAGTGCAGACACAACTATTCTGTTTGTAAAAGGAGAAG
  ATTTTCCGGCAAACAACATCGTGAAGTTCCTGGTTGGCTTTACAAACAAGGGGACAGAAGATTTTATTGT
  TGAATCGCTAGATGCCTCGTTCCGTTATCCTCAGGACTACCAGTTTTACATCCAGAATTTCACAGCTCTT
  CCTCTAAACACTGTAGTGCCACCCCAGCGGCAGGCAACTTTTGAGTATTCCTTCATCCCTGCAGAGCCGA
  TGGGAGGACGGCCCTTTGGTCTGGTTATCAATCTGAACTACAAAGATTTGAACGGCAATGTATTCCAGGA
  TGCTGTCTTCAATCAGACGGTCACAGTTATTGAAAGAGAGGACGGGTTAGATGGAGAAACAATTTTTATG
  TATATGTTCCTTGCTGGTCTTGGGCTTCTGGTTGTTGTTGGCCTTCATCAGCTCCTGGAATCTAGAAAGC
  GCAAGAGACCCATACAGAAAGTAGAAATGGGTACCTCAAGTCAGAATGATGTTGACATGAGCTGGATTCC
  TCAGGAGACTTTGAATCAGATCAATAAAGCTTCTCCCAGAAGGCAGCCCCGGAAGCGGGCGCAGAAGCGA
  TCGGTGGGATCTGATGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203901 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRLLPRLLLLFLLAFPAAVLLRGGPGGSLALAQDPTEDEEIVEDSIIEDEDDEAEVEEDEPTDLAEDKEE
  EDVSSEPEASPSADTTILFVKGEDFPANNIVKFLVGFTNKGTEDFIVESLDASFRYPQDYQFYIQNFTAL
  PLNTVVPPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDLNGNVFQDAVFNQTVTVIEREDGLDGETIFM
  YMFLAGLGLLVVVGLHQLLESRKRKRPIQKVEMGTSSQNDVDMSWIPQETLNQINKASPRRQPRKRAQKR
  SVGSDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025965
• ORF Size: 861 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025965.1, NM_025965.2, NM_025965.3, NP_080241.3
• RefSeq Size: 9306 bp
• RefSeq ORF: 861 bp
• Locus ID: 107513
• UniProt ID: Q9CY50
• Cytogenetics: 13 A3.3
• MW: 32.1 kDa
• Gene Summary: TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.[UniProtKB/Swiss-Prot Function]