Rrp15 (NM_026041) Mouse Tagged ORF Clone

CAT#: MR203800

Rrp15 (Myc-DDK-tagged) - Mouse ribosomal RNA processing 15 homolog (S. cerevisiae) (Rrp15)

Product Images

Circular map for MR203800

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rrp15
• Synonyms: 2810430M08Rik; 5430437H21Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203800 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGGCCGTTCAGGACTCACGTGTGAGCCCAGGGGAGATGCTGAAGCGTTCCCCGAAGAAGAAGA
  AAAAGATGAAGATGGTAGCCAAGGCTGCGGCGTCGAAGCTAGAAGATGAGGTCAAAGACTCTTCTGATGG
  AGAAGGAAGCTGTGACTCAGACATGGACCACTCAGATGATGGAGCTGCAGAAGCAGACAGTGAGGACAAT
  GTTGAATCCTGTGAGGAAGACAATGAAGACGCTGCAGAGTCGAGTGCTGGGACCAATTCAGGCTGGGCAG
  ATGCCATGGCAAAAATCCTTAACAAAAAGACTCCTAAAAGCAAAGCCACCATCCTCACCAAAAACAAAGA
  GCTGGAGAAGGAGAAGGAGAAGCTAAAGCAGGAGAGGCTGGAGAAAAGGAAGCAGCTTGATAAGAAGCGG
  GAGTGGGAAATGCTGTGCAGAGTGAAGCCAGATGTTGTCAAAGACAAAGAGGCAGAGAGGAACCTTCAGA
  GGATTGCGACAAGGGGTGTGGTGCAGCTCTTCAATGCTGTTCAGAAACACCAAAGGAATGTTGGCGAAAA
  GGTTAAGGAAGCGGGAGGCTCCGTCCGCAAGCGAGCCAAGCTGATGTCAACTGTTTCCAAGAAGGATTTC
  ATCAGTGTTCTTCGAGGAATGGATGGTACAAGTAGGAACAGTCCTGCTGGGAAGAGCCCCAAAGCCAGAC
  AGACTGAAGTGAAATCAGAAGAGAGCCCAGGGTGGAAGATTCTGCGAGATGATTTCATGATGGGAGCCAG
  CATGAAGGACTGGGACAAGGAGAGCGAGGGCGAGGAGCCTGCTGGTGGCAGGGCAGAGGCCGCAGCCAGC
  CGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203800 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAVQDSRVSPGEMLKRSPKKKKKMKMVAKAAASKLEDEVKDSSDGEGSCDSDMDHSDDGAAEADSEDN
  VESCEEDNEDAAESSAGTNSGWADAMAKILNKKTPKSKATILTKNKELEKEKEKLKQERLEKRKQLDKKR
  EWEMLCRVKPDVVKDKEAERNLQRIATRGVVQLFNAVQKHQRNVGEKVKEAGGSVRKRAKLMSTVSKKDF
  ISVLRGMDGTSRNSPAGKSPKARQTEVKSEESPGWKILRDDFMMGASMKDWDKESEGEEPAGGRAEAAAS
  R
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026041
• ORF Size: 846 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026041.2, NP_080317.3
• RefSeq Size: 1228 bp
• RefSeq ORF: 846 bp
• Locus ID: 67223
• UniProt ID: Q9CYX7
• Cytogenetics: 1 H5
• MW: 31.1 kDa
• Gene Summary: This gene encodes a protein similar to budding yeast Rrp15p. Rrp15p is a component of pre-60S ribosomal particles in budding yeast, and is required for the early maturation steps of the 60S subunit. The mouse genome contains at least one pseudogene on the X chromosome. [provided by RefSeq, Jul 2008]