Itm2c (NM_022417) Mouse Tagged ORF Clone

CAT#: MR203562

Itm2c (Myc-DDK-tagged) - Mouse integral membrane protein 2C (Itm2c)

Product Images

Circular map for MR203562

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Itm2c
• Synonyms: 3110038L02Rik; BRI3; Bricd2c; E25; E25C; ITM3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203562 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAAGATCAGTTTCCAGCCCGCGGTAGCGGGCATCAAGGCTGATAAAGCGGACAAAGCAGCGGCGT
  CTGGTCCGGCCTCGGCATCTGCCCCGGCCGCGGAGATCCTGCTGACGCCGGCGAGGGAGGAACGCCCCCC
  TCGCCACCGCTCCCGGAAAGGAGGCTCCGTGGGCGGCGTGTGCTACCTGTCCATGGGCATGGTTGTATTG
  CTCATGGGCCTTGTGTTTGCCTCGGTCTACATCTACAGGTACTTCTTCCTTGCTCAGCTGGCCCGAGATA
  ACTTCTTCCACTGTGGTGTGCTCTACGAAGACTCCCTGTCCTCCCAGATCCGGACTCGCCTGGAGCTGGA
  AGAGGATGTGAAGATCTATCTTGAGGAGAACTATGAACGTATTAATGTCCCTGTGCCCCAGTTTGGTGGT
  GGGGACCCTGCAGACATCATCCATGACTTCCAGCGGGGTCTCACTGCCTACCACGACATCTCCCTAGACA
  AGTGCTACGTCATCGAGCTCAACACCACCATCGTGCTACCCCCACGAAACTTCTGGGAGCTCCTCATGAA
  CGTGAAGAGAGGGACCTACCTCCCACAGACGTACATCATCCAGGAGGAGATGGTGGTGACAGAGCATGTC
  CGCGACAAGGAGGCTCTGGGTTCCTTCATCTACCACCTGTGCAACGGGAAGGACACCTACCGGCTGCGGC
  GCCGGTCTACCCGGAGGCGGATCAACAAACGTGGGGCCAAGAACTGCAACGCCATCCGCCACTTCGAGAA
  TACATTTGTGGTGGAGACGCTCATCTGTGGGGTGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203562 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVKISFQPAVAGIKADKADKAAASGPASASAPAAEILLTPAREERPPRHRSRKGGSVGGVCYLSMGMVVL
  LMGLVFASVYIYRYFFLAQLARDNFFHCGVLYEDSLSSQIRTRLELEEDVKIYLEENYERINVPVPQFGG
  GDPADIIHDFQRGLTAYHDISLDKCYVIELNTTIVLPPRNFWELLMNVKRGTYLPQTYIIQEEMVVTEHV
  RDKEALGSFIYHLCNGKDTYRLRRRSTRRRINKRGAKNCNAIRHFENTFVVETLICGVV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022417
• ORF Size: 810 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022417.1, NM_022417.2, NM_022417.3, NP_071862.1, NP_071862.2
• RefSeq Size: 2024 bp
• RefSeq ORF: 810 bp
• Locus ID: 64294
• UniProt ID: Q91VK4
• Cytogenetics: 1 C5
• MW: 30.5 kDa
• Gene Summary: Negative regulator of amyloid-beta peptide production. May inhibit the processing of APP by blocking its access to alpha- and beta-secretase. Binding to the beta-secretase-cleaved APP C-terminal fragment is negligible, suggesting that ITM2C is a poor gamma-secretase cleavage inhibitor. May play a role in TNF-induced cell death and neuronal differentiation.[UniProtKB/Swiss-Prot Function]